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The map label for this gene is pinE [H]

Identifier: 154245047

GI number: 154245047

Start: 1269967

End: 1270605

Strand: Reverse

Name: pinE [H]

Synonym: Xaut_1099

Alternate gene names: 154245047

Gene position: 1270605-1269967 (Counterclockwise)

Preceding gene: 154245048

Following gene: 154245046

Centisome position: 23.93

GC content: 69.64

Gene sequence:

>639_bases
GTGTCCCGCAAGACTGCTCGCCGCGTCGCCCTCTACCTGCGCGTCTCCACCTCCGAACAGACCACAGAGAACCAGCGTCG
CGAGCTTCGGGCCGTGGCGGAAAAGGCGGGATGGGAGGTGGTGGCCGTGTTCGAGGATGCCGGCATCTCCGGCGCCAAGG
GCCGCGACCAGCGCCCCGGCTACGACGCCATGCTGAAGGCCGTCACCCGCCGCGAGGTGGACATGGTTGCTGCGTGGTCG
GTGGACCGGCTGGGCCGTTCGATGGCGGACCTCGTGGCCTTCCTCGGCGAGCTTCACGCCCGCGGCGCCGACCTCTACCT
GCACCAGCAAGCCCTCGACACCACCACGCCCTCCGGCCGGGCCATGTTCCAGATGATGGGCGTGTTCGCCGAGTACGAGC
GGGCCATGATCCGGGAGCGCGTGAACGCCGGGCTGGCGCGAGCGAAGGCGGAGGGCAAGAAGCTGGGGCGGCCCACCGTG
GGGGCCGAGACCGAGGCTCGGATCAAGGAGCTTCGGGCGCATGGAATGGGGATGCTGAAGGTGGCGAAGACGCTTGGAGT
GGGGGTGTCGGCGGTGCAGAGGGTGGTCGGGAGCGGGAGCGACCGTCTCACCCGTGACAGCACACAACCGCAGCGTTAG

Upstream 100 bases:

>100_bases
CCCGAGGCACCGATCCATGCAGGTTTGCGAAGGGCGCTTGACTCATTCCGTAAACAATTGTTATCGGTATGGCCATCACT
AGCTTCCCGGAGCCCCTGCC

Downstream 100 bases:

>100_bases
ACATTGCCCTACCGTTCTGGAAGATTGAGCAGCGAATGGTCGGCCGGCGAGGGGGGAATGGGCTCGGTCAACTTCGGCTT
TCTCGACGCGCACGACGCCA

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSRKTARRVALYLRVSTSEQTTENQRRELRAVAEKAGWEVVAVFEDAGISGAKGRDQRPGYDAMLKAVTRREVDMVAAWS
VDRLGRSMADLVAFLGELHARGADLYLHQQALDTTTPSGRAMFQMMGVFAEYERAMIRERVNAGLARAKAEGKKLGRPTV
GAETEARIKELRAHGMGMLKVAKTLGVGVSAVQRVVGSGSDRLTRDSTQPQR

Sequences:

>Translated_212_residues
MSRKTARRVALYLRVSTSEQTTENQRRELRAVAEKAGWEVVAVFEDAGISGAKGRDQRPGYDAMLKAVTRREVDMVAAWS
VDRLGRSMADLVAFLGELHARGADLYLHQQALDTTTPSGRAMFQMMGVFAEYERAMIRERVNAGLARAKAEGKKLGRPTV
GAETEARIKELRAHGMGMLKVAKTLGVGVSAVQRVVGSGSDRLTRDSTQPQR
>Mature_211_residues
SRKTARRVALYLRVSTSEQTTENQRRELRAVAEKAGWEVVAVFEDAGISGAKGRDQRPGYDAMLKAVTRREVDMVAAWSV
DRLGRSMADLVAFLGELHARGADLYLHQQALDTTTPSGRAMFQMMGVFAEYERAMIRERVNAGLARAKAEGKKLGRPTVG
AETEARIKELRAHGMGMLKVAKTLGVGVSAVQRVVGSGSDRLTRDSTQPQR

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=189, Percent_Identity=40.7407407407407, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1787638, Length=192, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787827, Length=159, Percent_Identity=35.8490566037736, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 23276; Mature: 23145

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKTARRVALYLRVSTSEQTTENQRRELRAVAEKAGWEVVAVFEDAGISGAKGRDQRPG
CCCHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
YDAMLKAVTRREVDMVAAWSVDRLGRSMADLVAFLGELHARGADLYLHQQALDTTTPSGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCCCHH
AMFQMMGVFAEYERAMIRERVNAGLARAKAEGKKLGRPTVGAETEARIKELRAHGMGMLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHH
VAKTLGVGVSAVQRVVGSGSDRLTRDSTQPQR
HHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRKTARRVALYLRVSTSEQTTENQRRELRAVAEKAGWEVVAVFEDAGISGAKGRDQRPG
CCHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
YDAMLKAVTRREVDMVAAWSVDRLGRSMADLVAFLGELHARGADLYLHQQALDTTTPSGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCCCHH
AMFQMMGVFAEYERAMIRERVNAGLARAKAEGKKLGRPTVGAETEARIKELRAHGMGMLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHH
VAKTLGVGVSAVQRVVGSGSDRLTRDSTQPQR
HHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]