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The map label for this gene is pinE [H]

Identifier: 154245039

GI number: 154245039

Start: 1257577

End: 1258056

Strand: Direct

Name: pinE [H]

Synonym: Xaut_1090

Alternate gene names: 154245039

Gene position: 1257577-1258056 (Clockwise)

Preceding gene: 154245036

Following gene: 154245040

Centisome position: 23.69

GC content: 65.83

Gene sequence:

>480_bases
TTGGGTTGCCAGAAAGTCTGTTCTGAGACGGCCGGCGGCGCGAAGACCGATCGGCGCGAGTTGCGGCTCTGCATCGCCTC
GCTCGCAGCCGGCGACGTTCTCGTCGTCGTTAGGCTCGATCGCCTCGCGAGGTCCACGCGGGATCTGCTCAACGTGCTTG
CCGAGGTCACTGAACGCCACGCCGCCTTTCGCTCGCTCTCAGACGCGTGGGCCGATACCACCACGCCCCATGGGCGGCTG
ATGCTAACCGTGCTAGGCGGCCTGGCCGAATTCCAGCGCGAACTCATCAAGACGCGAACGGGGGAAGGACGGGCGCGAAC
CCAGGCGCGAGGCGTGAAGTTCGGCCGCAAGAGCGTGCTTGCGGTCGATCAGCGCGCCTATGTCGCCAAGTTGCGCGCCG
AAGGCGAAAGCGTGCGGCACATCGCCCGCATCATGGGCGTCAGCAAGGCAACCATTAGCCGGATTGCTCCGGTCTCGTAG

Upstream 100 bases:

>100_bases
CTCGAATTATAGTCTCATCATGTGTGCATGATCATCGCGCTATGCACGCGTATCGACTGATGGACAGACGCTAGGCAGCC
AGATCGAACACCTCAAAACC

Downstream 100 bases:

>100_bases
GGAATGGTTGCGCCAGAGGCGAACGCGCCGGCTTCGATCCCCCGCTGGAATGATACCCCACGCGAACGCGGGGAAATGTT
CGCCAAGCGCGCTGCCCCCC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MGCQKVCSETAGGAKTDRRELRLCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEVTERHAAFRSLSDAWADTTTPHGRL
MLTVLGGLAEFQRELIKTRTGEGRARTQARGVKFGRKSVLAVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIAPVS

Sequences:

>Translated_159_residues
MGCQKVCSETAGGAKTDRRELRLCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEVTERHAAFRSLSDAWADTTTPHGRL
MLTVLGGLAEFQRELIKTRTGEGRARTQARGVKFGRKSVLAVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIAPVS
>Mature_158_residues
GCQKVCSETAGGAKTDRRELRLCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEVTERHAAFRSLSDAWADTTTPHGRLM
LTVLGGLAEFQRELIKTRTGEGRARTQARGVKFGRKSVLAVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIAPVS

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=156, Percent_Identity=39.7435897435897, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1787638, Length=153, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1787827, Length=153, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 17297; Mature: 17166

Theoretical pI: Translated: 11.39; Mature: 11.39

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCQKVCSETAGGAKTDRRELRLCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEVTERH
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFRSLSDAWADTTTPHGRLMLTVLGGLAEFQRELIKTRTGEGRARTQARGVKFGRKSVL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
AVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIAPVS
HHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
GCQKVCSETAGGAKTDRRELRLCIASLAAGDVLVVVRLDRLARSTRDLLNVLAEVTERH
CHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFRSLSDAWADTTTPHGRLMLTVLGGLAEFQRELIKTRTGEGRARTQARGVKFGRKSVL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHH
AVDQRAYVAKLRAEGESVRHIARIMGVSKATISRIAPVS
HHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]