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The map label for this gene is gatA [H]

Identifier: 154245002

GI number: 154245002

Start: 1205752

End: 1206861

Strand: Direct

Name: gatA [H]

Synonym: Xaut_1052

Alternate gene names: 154245002

Gene position: 1205752-1206861 (Clockwise)

Preceding gene: 154245001

Following gene: 154245004

Centisome position: 22.71

GC content: 68.83

Gene sequence:

>1110_bases
ATGAGCATCTTTGTCAAGACCCTCAGCCTCGGGGCGGATGGCCCACGCGTCGCAGTGAAGGACACCATCGATATCGCGGG
CGAGCCGACCATGGCTGGCAGCCGGGCCCTGGCCGACGCAGCCCCCGCGACCGAACATGCCGAAGTGGTGGCGCGGGCGC
TCGCAGCGGGCTGCCGGATCGTCGGCAAGACCACGCTCCACGAGCTGGCCTTCGGCGTGACCGGCATCAACGACTGGAGC
GGAACGCCCGAGAACCCGAAATGGCCGCATCTGGTGCCCGGCGGCTCGTCCTCGGGATCGGCCGCGGCAGTGGCGGCGGG
GCTCGCCGATTTCGCCCTCGGCACGGACACCGGCGGGTCCGTCCGCATCCCGGCCGCCTGCTGCGGTGTCTTCGGGCTGA
AGCCGACCTTCGGTCGGGTCAGCCGGCACGGTGTGATGCCAACCGACAGCACCATCGACTGCGTAGGCCCGATCGCGACA
AGCGCGGACATGCTGATGCGCGCCATGGAGATCATCGATCCCACCTTCGCGCGACGGTCGGTCAATGCGCCGCGCATCGG
GCGCGTGGCGGTGAAGGCCAATCCGGCGATCCACCGGGCCGTCAACGAGGCACTGGCAGCTTCGGGCGTCGAGAGCCGCG
ATGTCAAACTCGCCGGACTTGAAGCCGCCTTCTCCGCCGGTCTCGCAATCATCAATGCCGAGAACGTCACCGGCCTCGGC
CACCTGCTGCAGACGGGTCTCGTGGGAGACGACGTCGCCCGGCGCCTCGCCCAGGCAGCCGCCGTAACCAGGGAGGAGAT
CGCCGCAGCCGAGTTTGTGCGTGCGGCGTTCACGGCGGAGATCGACGCACTCCTCGAAGCCCTCGACGTACTGGTTCTGC
CGACCATGCCCGAGTTCCCGCCTGCTCTCGCGGAAGCGCGCGGCGACCTTTCCGCCGTGGGCATGACGACGTTCGTGCGC
CCCTTCAATCTGTCGGGACATCCCGCCGTGACCGTGCCGCTGCTGGCAGAGAACGGCGCGCCGGCGGGTTTGCAGATCGT
CGGGAGAAAGGGCGAGGATGCGTTGGTCCTGGCGGTCGCTGAACGGGTCAGCCTCGCAAGGTCGCAATGA

Upstream 100 bases:

>100_bases
GCGCGCCTTCCTGACTGCCCACGGCGCCGATCCTGCGGCCATCTTTCTGGAACGTTTTGTCGTGAGCGGCGCCACCGCCG
CCTGATCGAAAGCTTGATCC

Downstream 100 bases:

>100_bases
GGGTCGATTTCAGCCCCAGTTGTTTCCACCAAAAATGATACATTAGTTTAAAAAATGACGACTACGTAAAATATATTGCT
TGAATTCACTCCAAATTACT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSIFVKTLSLGADGPRVAVKDTIDIAGEPTMAGSRALADAAPATEHAEVVARALAAGCRIVGKTTLHELAFGVTGINDWS
GTPENPKWPHLVPGGSSSGSAAAVAAGLADFALGTDTGGSVRIPAACCGVFGLKPTFGRVSRHGVMPTDSTIDCVGPIAT
SADMLMRAMEIIDPTFARRSVNAPRIGRVAVKANPAIHRAVNEALAASGVESRDVKLAGLEAAFSAGLAIINAENVTGLG
HLLQTGLVGDDVARRLAQAAAVTREEIAAAEFVRAAFTAEIDALLEALDVLVLPTMPEFPPALAEARGDLSAVGMTTFVR
PFNLSGHPAVTVPLLAENGAPAGLQIVGRKGEDALVLAVAERVSLARSQ

Sequences:

>Translated_369_residues
MSIFVKTLSLGADGPRVAVKDTIDIAGEPTMAGSRALADAAPATEHAEVVARALAAGCRIVGKTTLHELAFGVTGINDWS
GTPENPKWPHLVPGGSSSGSAAAVAAGLADFALGTDTGGSVRIPAACCGVFGLKPTFGRVSRHGVMPTDSTIDCVGPIAT
SADMLMRAMEIIDPTFARRSVNAPRIGRVAVKANPAIHRAVNEALAASGVESRDVKLAGLEAAFSAGLAIINAENVTGLG
HLLQTGLVGDDVARRLAQAAAVTREEIAAAEFVRAAFTAEIDALLEALDVLVLPTMPEFPPALAEARGDLSAVGMTTFVR
PFNLSGHPAVTVPLLAENGAPAGLQIVGRKGEDALVLAVAERVSLARSQ
>Mature_368_residues
SIFVKTLSLGADGPRVAVKDTIDIAGEPTMAGSRALADAAPATEHAEVVARALAAGCRIVGKTTLHELAFGVTGINDWSG
TPENPKWPHLVPGGSSSGSAAAVAAGLADFALGTDTGGSVRIPAACCGVFGLKPTFGRVSRHGVMPTDSTIDCVGPIATS
ADMLMRAMEIIDPTFARRSVNAPRIGRVAVKANPAIHRAVNEALAASGVESRDVKLAGLEAAFSAGLAIINAENVTGLGH
LLQTGLVGDDVARRLAQAAAVTREEIAAAEFVRAAFTAEIDALLEALDVLVLPTMPEFPPALAEARGDLSAVGMTTFVRP
FNLSGHPAVTVPLLAENGAPAGLQIVGRKGEDALVLAVAERVSLARSQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=430, Percent_Identity=29.5348837209302, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI195972892, Length=405, Percent_Identity=23.9506172839506, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=172, Percent_Identity=37.7906976744186, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=27.9904306220096, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319685, Length=384, Percent_Identity=29.4270833333333, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648113, Length=426, Percent_Identity=28.169014084507, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356731, Length=390, Percent_Identity=25.8974358974359, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648435, Length=393, Percent_Identity=24.6819338422392, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648437, Length=393, Percent_Identity=24.6819338422392, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648441, Length=394, Percent_Identity=25.6345177664975, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648439, Length=394, Percent_Identity=25.6345177664975, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550774, Length=393, Percent_Identity=24.6819338422392, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161078093, Length=317, Percent_Identity=27.1293375394322, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 37651; Mature: 37519

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFVKTLSLGADGPRVAVKDTIDIAGEPTMAGSRALADAAPATEHAEVVARALAAGCRI
CEEEEEEEECCCCCCEEEEEECEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCEE
VGKTTLHELAFGVTGINDWSGTPENPKWPHLVPGGSSSGSAAAVAAGLADFALGTDTGGS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
VRIPAACCGVFGLKPTFGRVSRHGVMPTDSTIDCVGPIATSADMLMRAMEIIDPTFARRS
EECCHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHC
VNAPRIGRVAVKANPAIHRAVNEALAASGVESRDVKLAGLEAAFSAGLAIINAENVTGLG
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCEEEEECCCCCHHH
HLLQTGLVGDDVARRLAQAAAVTREEIAAAEFVRAAFTAEIDALLEALDVLVLPTMPEFP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PALAEARGDLSAVGMTTFVRPFNLSGHPAVTVPLLAENGAPAGLQIVGRKGEDALVLAVA
HHHHHHCCCHHHHHHHHHEEECCCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEHHH
ERVSLARSQ
HHHHHHCCC
>Mature Secondary Structure 
SIFVKTLSLGADGPRVAVKDTIDIAGEPTMAGSRALADAAPATEHAEVVARALAAGCRI
EEEEEEEECCCCCCEEEEEECEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHCCEE
VGKTTLHELAFGVTGINDWSGTPENPKWPHLVPGGSSSGSAAAVAAGLADFALGTDTGGS
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCC
VRIPAACCGVFGLKPTFGRVSRHGVMPTDSTIDCVGPIATSADMLMRAMEIIDPTFARRS
EECCHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHC
VNAPRIGRVAVKANPAIHRAVNEALAASGVESRDVKLAGLEAAFSAGLAIINAENVTGLG
CCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCEEEEECCCCCHHH
HLLQTGLVGDDVARRLAQAAAVTREEIAAAEFVRAAFTAEIDALLEALDVLVLPTMPEFP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PALAEARGDLSAVGMTTFVRPFNLSGHPAVTVPLLAENGAPAGLQIVGRKGEDALVLAVA
HHHHHHCCCHHHHHHHHHEEECCCCCCCEEEEEEEECCCCCCCEEEEECCCCCEEEEHHH
ERVSLARSQ
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]