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The map label for this gene is livM [H]

Identifier: 154244997

GI number: 154244997

Start: 1199653

End: 1200597

Strand: Direct

Name: livM [H]

Synonym: Xaut_1047

Alternate gene names: 154244997

Gene position: 1199653-1200597 (Clockwise)

Preceding gene: 154244996

Following gene: 154244998

Centisome position: 22.6

GC content: 68.68

Gene sequence:

>945_bases
ATGGTCCGCATCCCCCTGCTCATCGCCATCCTCGTGGCCTGCCTGGTCACGGGCGGCGACTATGCCATCAGCCTCGCAGT
GGTCATCGGACTGCAGGCACTGCCCGCCCTCGGCCTGACGCTCCTCACCGGCTACACCGGCCAGATCTCGCTCGGCCATG
CCGCCTTCTACGGCCTCGGCGCCTACGGCTCCGCGCTTATCGGCGCCGCCCTCGGCCTGCCCGCCCTGCTGAGCGTGATC
ATTGCCTCGGCGCTGGTGGCGGGGATCGCCTGGGCCATCGGCTGGCTGGTGTTCCGGCTGAAGGGGCACCATCTCGCCAT
GGCGACGCTGGCCTTCGGCATCATCGTGCAGATCGCCTTCGTCGAGATGCATTTCCTCACCGGCGGTCCCAACGGCCTGT
CGGGCATCGAGCCGCTGTCGATCTTCGGCAAGGACCTCATCACCGACGCCCAGGTGCTGCCGGTGGTGTGGGGCGCGGTG
ATCCTCGCGGTCCTGTTCGCCCAGAACCTCATCTCCTCACCCGCCGGCCTCGTGATGCGGGCCATCGCGGAGGGCGACAA
GGTGGTCGGCTCTCTCGGCAATGACGTCGCCGCTATCAAGCGCAAGGTGATGATGCTGAGCGGCTTCCTCTGCGCGGTGG
GCGGCGGGCTCTACGCCCATTATGTCGGCTACCTCAGCCCCGGACCGTTCGACGTGGGCTTCTCCGTGCGGCTCCTGCTC
ATGGTGGCGCTCGGCGGTTTCGCCAACATCTGGGGCGTGCTGTTCGGCGTCGCCTTCGTGACGCTGGTGGGCGAGGCCCT
GAAGCCGCTCGGCGCCTTCGACGTGATCGCCTACGGCACGCTCCTCGTGGTCTCGGTCATCTTCTTCCCGCGCGGCCTCA
TGGCCGGCATCGCGGAGCCCGTGGTGGCGCAGGCGCGCCGGTTCCGAAAGGTGGCCGCCCGATGA

Upstream 100 bases:

>100_bases
TCCGGCTACAAGAACGCCATCGCCTTCGTGCTGCTGCTCGTCTTCCTGCTGTTCCGGCCCGGCGGCCTGTTCGGCGACCT
CGAAAAGGTGAGGAGCTGAC

Downstream 100 bases:

>100_bases
ACGCCAAGCCGATCCTCTCCGTCGCCGGTGTCAGCGTCACCTTCGGGACGTTTCCGGCCCTCACCGAGGTGACCTTCGAC
GTGGCCGAAGGGGAACTGGT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MVRIPLLIAILVACLVTGGDYAISLAVVIGLQALPALGLTLLTGYTGQISLGHAAFYGLGAYGSALIGAALGLPALLSVI
IASALVAGIAWAIGWLVFRLKGHHLAMATLAFGIIVQIAFVEMHFLTGGPNGLSGIEPLSIFGKDLITDAQVLPVVWGAV
ILAVLFAQNLISSPAGLVMRAIAEGDKVVGSLGNDVAAIKRKVMMLSGFLCAVGGGLYAHYVGYLSPGPFDVGFSVRLLL
MVALGGFANIWGVLFGVAFVTLVGEALKPLGAFDVIAYGTLLVVSVIFFPRGLMAGIAEPVVAQARRFRKVAAR

Sequences:

>Translated_314_residues
MVRIPLLIAILVACLVTGGDYAISLAVVIGLQALPALGLTLLTGYTGQISLGHAAFYGLGAYGSALIGAALGLPALLSVI
IASALVAGIAWAIGWLVFRLKGHHLAMATLAFGIIVQIAFVEMHFLTGGPNGLSGIEPLSIFGKDLITDAQVLPVVWGAV
ILAVLFAQNLISSPAGLVMRAIAEGDKVVGSLGNDVAAIKRKVMMLSGFLCAVGGGLYAHYVGYLSPGPFDVGFSVRLLL
MVALGGFANIWGVLFGVAFVTLVGEALKPLGAFDVIAYGTLLVVSVIFFPRGLMAGIAEPVVAQARRFRKVAAR
>Mature_314_residues
MVRIPLLIAILVACLVTGGDYAISLAVVIGLQALPALGLTLLTGYTGQISLGHAAFYGLGAYGSALIGAALGLPALLSVI
IASALVAGIAWAIGWLVFRLKGHHLAMATLAFGIIVQIAFVEMHFLTGGPNGLSGIEPLSIFGKDLITDAQVLPVVWGAV
ILAVLFAQNLISSPAGLVMRAIAEGDKVVGSLGNDVAAIKRKVMMLSGFLCAVGGGLYAHYVGYLSPGPFDVGFSVRLLL
MVALGGFANIWGVLFGVAFVTLVGEALKPLGAFDVIAYGTLLVVSVIFFPRGLMAGIAEPVVAQARRFRKVAAR

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=301, Percent_Identity=28.2392026578073, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 32366; Mature: 32366

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIPLLIAILVACLVTGGDYAISLAVVIGLQALPALGLTLLTGYTGQISLGHAAFYGLG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHH
AYGSALIGAALGLPALLSVIIASALVAGIAWAIGWLVFRLKGHHLAMATLAFGIIVQIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VEMHFLTGGPNGLSGIEPLSIFGKDLITDAQVLPVVWGAVILAVLFAQNLISSPAGLVMR
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIAEGDKVVGSLGNDVAAIKRKVMMLSGFLCAVGGGLYAHYVGYLSPGPFDVGFSVRLLL
HHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHH
MVALGGFANIWGVLFGVAFVTLVGEALKPLGAFDVIAYGTLLVVSVIFFPRGLMAGIAEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAQARRFRKVAAR
HHHHHHHHHHHHCC
>Mature Secondary Structure
MVRIPLLIAILVACLVTGGDYAISLAVVIGLQALPALGLTLLTGYTGQISLGHAAFYGLG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHH
AYGSALIGAALGLPALLSVIIASALVAGIAWAIGWLVFRLKGHHLAMATLAFGIIVQIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VEMHFLTGGPNGLSGIEPLSIFGKDLITDAQVLPVVWGAVILAVLFAQNLISSPAGLVMR
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIAEGDKVVGSLGNDVAAIKRKVMMLSGFLCAVGGGLYAHYVGYLSPGPFDVGFSVRLLL
HHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHH
MVALGGFANIWGVLFGVAFVTLVGEALKPLGAFDVIAYGTLLVVSVIFFPRGLMAGIAEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVAQARRFRKVAAR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]