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The map label for this gene is yfcG [H]

Identifier: 154244931

GI number: 154244931

Start: 1096669

End: 1097379

Strand: Direct

Name: yfcG [H]

Synonym: Xaut_0981

Alternate gene names: 154244931

Gene position: 1096669-1097379 (Clockwise)

Preceding gene: 154244927

Following gene: 154244932

Centisome position: 20.66

GC content: 68.64

Gene sequence:

>711_bases
TTGATCCGCTTCTACTTCCATCCCACGCCCAATCCCGCCAAGGTCGCCCTGTTCCTGGAGGAGGCAGGCCTCCCCTACGA
GGTGGTGCCCGTCGACACGGCGAAGGGCGAGCAGCATCTGCCGGCGTTCCGCGCCATCAATCCCAACGGCAAGGTGCCGG
CCATCGTCGATACCGAGGGTCCCGGCGGCCGCGAGGCGCGGGTGTTCGATTCCACCGCCATCCTGCTCTATCTCGCGGAG
AAGACGGGGCAGTTCCTGGGCGCGCCGCAGGACCGGCCGGATCTGTTGTCCTGGCTGCTCTTCATCGCCTCGGGGCTCGG
CCCGTTCTCGGGGCAGGCGGTGCATTTCCAGCATGCGGCGCCGCAGGGCCTCGACTATGCCGTGAACCGCTACCGGCGCG
AGGTTGAGCGGCACTACAAGGTGCTCGACGACCATCTTGCCACCCGCCGCTTCATCGTGGGCGAGACCTACACCATCGCC
GACATGTCGGCCTGGGGCTGGCTCGATCGCGCCGCGCGGGTGCTGAAGGTGGAGAGCGATCCGCTCGCCGGTTTCCCCAA
CCTGAAGCGCTGGTTCGCGCAGATCGATGCGCGCGAGGCGGTGGCGCGGGCTCGCGCGGTGGGCAAGGACCACAGCTTCA
AGGCGTCCGGCGACGAGGAAAGCCGCCGCGCCCTGTTCCCCTCCAACTATCCGCCCGCAGCCGCGGAGTGA

Upstream 100 bases:

>100_bases
GCTTGCGGTCCCCGCGTTGCCGTGCATTCTATATCAGTCGATCTAAAATGCAAATGCGCGTCGGCCCCCAGGGGTCGGCC
CGCCCGGCAAAGGTGATCCC

Downstream 100 bases:

>100_bases
GGTGGCAGTCGCGTCCGGTCTCCTGAAGGAGGAAGCCATGAAGATCGAAGGAAAGCGGGTCCTCGTCACCGGGGGCTCGA
GCGGAATCGGCCGCGCCATC

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MIRFYFHPTPNPAKVALFLEEAGLPYEVVPVDTAKGEQHLPAFRAINPNGKVPAIVDTEGPGGREARVFDSTAILLYLAE
KTGQFLGAPQDRPDLLSWLLFIASGLGPFSGQAVHFQHAAPQGLDYAVNRYRREVERHYKVLDDHLATRRFIVGETYTIA
DMSAWGWLDRAARVLKVESDPLAGFPNLKRWFAQIDAREAVARARAVGKDHSFKASGDEESRRALFPSNYPPAAAE

Sequences:

>Translated_236_residues
MIRFYFHPTPNPAKVALFLEEAGLPYEVVPVDTAKGEQHLPAFRAINPNGKVPAIVDTEGPGGREARVFDSTAILLYLAE
KTGQFLGAPQDRPDLLSWLLFIASGLGPFSGQAVHFQHAAPQGLDYAVNRYRREVERHYKVLDDHLATRRFIVGETYTIA
DMSAWGWLDRAARVLKVESDPLAGFPNLKRWFAQIDAREAVARARAVGKDHSFKASGDEESRRALFPSNYPPAAAE
>Mature_236_residues
MIRFYFHPTPNPAKVALFLEEAGLPYEVVPVDTAKGEQHLPAFRAINPNGKVPAIVDTEGPGGREARVFDSTAILLYLAE
KTGQFLGAPQDRPDLLSWLLFIASGLGPFSGQAVHFQHAAPQGLDYAVNRYRREVERHYKVLDDHLATRRFIVGETYTIA
DMSAWGWLDRAARVLKVESDPLAGFPNLKRWFAQIDAREAVARARAVGKDHSFKASGDEESRRALFPSNYPPAAAE

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=46.1165048543689, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI87082195, Length=227, Percent_Identity=37.8854625550661, Blast_Score=131, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324100, Length=243, Percent_Identity=31.2757201646091, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26213; Mature: 26213

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFYFHPTPNPAKVALFLEEAGLPYEVVPVDTAKGEQHLPAFRAINPNGKVPAIVDTEG
CEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECCC
PGGREARVFDSTAILLYLAEKTGQFLGAPQDRPDLLSWLLFIASGLGPFSGQAVHFQHAA
CCCCCEEEECCEEEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCC
PQGLDYAVNRYRREVERHYKVLDDHLATRRFIVGETYTIADMSAWGWLDRAARVLKVESD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCHHHHHHHHHHHEEEECCC
PLAGFPNLKRWFAQIDAREAVARARAVGKDHSFKASGDEESRRALFPSNYPPAAAE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIRFYFHPTPNPAKVALFLEEAGLPYEVVPVDTAKGEQHLPAFRAINPNGKVPAIVDTEG
CEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECCC
PGGREARVFDSTAILLYLAEKTGQFLGAPQDRPDLLSWLLFIASGLGPFSGQAVHFQHAA
CCCCCEEEECCEEEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCC
PQGLDYAVNRYRREVERHYKVLDDHLATRRFIVGETYTIADMSAWGWLDRAARVLKVESD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCHHHHHHHHHHHEEEECCC
PLAGFPNLKRWFAQIDAREAVARARAVGKDHSFKASGDEESRRALFPSNYPPAAAE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]