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The map label for this gene is yafC [H]

Identifier: 154244922

GI number: 154244922

Start: 1089482

End: 1090381

Strand: Reverse

Name: yafC [H]

Synonym: Xaut_0972

Alternate gene names: 154244922

Gene position: 1090381-1089482 (Counterclockwise)

Preceding gene: 154244928

Following gene: 154244920

Centisome position: 20.54

GC content: 70.44

Gene sequence:

>900_bases
ATGGACACGGCCCAGCTGCGCATTTTTGTCCATGTGGCGAGACGGGGTAGCTTCGCCGCTGCCGCCCGCGACCTCGACAT
GGACGCTTCCGCCGTCTCCCGTGCCGTGGCGGCGCTGGAGGCGGAGCTTGACGCCCGGCTGATGCACCGGACCACGCGCG
CCATGTCGCTCACCGAGGCGGGGCAGGCGTACCTTGCCCGCGTGGAGCCCATCCTCGACGAGCTCGACCGCGCCCGCGAC
GAGGTGGCGTCCGCACGCGCGGAGCCGGTTGGCACACTCCGGCTGACAGCCTCCGTCGCCTTCGGGGAGGCCTGCCTGAT
GCCGCTGCTGGCGGAGATGCGCACGCGCTTTCCCCGCCTCAAGCTCGATCTGATCCTCACCGACACCAACCTCGACCTTG
CCACCGAGCGGATCGACATGGCGCTCCGGCTGGCGCCGGACGTGCGGGCGGACGTGATTGGCATAAGGCTGTTCGCCACC
CGGTACCGGGTGGTGGCGAGCCCGGATTATTGCGCGCGGGAGGGCGCCCCGCGCACCCCGCAGGAGGTCGGGAAGCATTG
CGCCATCGTGTTCGGCCTGCCGGACTTCCGCCTGCGCTGGATGTTTCGCCGCGACGGCCAGGTGGAGGAGGTCGCGGTGC
GCGGCGATATCGCCATTTCGAGCGGCCTCGCCATCCGCCGCGCGGCCCGCGACGGGCTCGGGCTCGCGCTGCTACCGGAC
TGGCTGGTGGGGCCGGACCTTGCCACCGGCACGCTCATCGACCTCTTTCCCGACTACGACATCACCGCCACCTCGTTCGA
TACCGCCGCCTGGCTCATCTATGCCAGCCGGCGCTATACGCCGAAGAAGGTGCGGCTCATGACGAAGTTCCTGCGCGAGC
GCCTCGGCAGGCCCGGCTGA

Upstream 100 bases:

>100_bases
TTCATGCCAAGATACCTCCAAAAGCGGAGGCAAGATGGCGCGCGCCCACGGCGGGGAAAAATGGGGACTGCGACAGTACA
TTCGTGCATAAGCTGCACGA

Downstream 100 bases:

>100_bases
GCGCAGACCGTTCAGGCCCGGCCCGAGGAGACCGGCAGGGCGGCGAGCCGTGCCCTCAGGCGGGGCACCACGAAATCCAC
GAAGGCGCGCAGTTTCGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MDTAQLRIFVHVARRGSFAAAARDLDMDASAVSRAVAALEAELDARLMHRTTRAMSLTEAGQAYLARVEPILDELDRARD
EVASARAEPVGTLRLTASVAFGEACLMPLLAEMRTRFPRLKLDLILTDTNLDLATERIDMALRLAPDVRADVIGIRLFAT
RYRVVASPDYCAREGAPRTPQEVGKHCAIVFGLPDFRLRWMFRRDGQVEEVAVRGDIAISSGLAIRRAARDGLGLALLPD
WLVGPDLATGTLIDLFPDYDITATSFDTAAWLIYASRRYTPKKVRLMTKFLRERLGRPG

Sequences:

>Translated_299_residues
MDTAQLRIFVHVARRGSFAAAARDLDMDASAVSRAVAALEAELDARLMHRTTRAMSLTEAGQAYLARVEPILDELDRARD
EVASARAEPVGTLRLTASVAFGEACLMPLLAEMRTRFPRLKLDLILTDTNLDLATERIDMALRLAPDVRADVIGIRLFAT
RYRVVASPDYCAREGAPRTPQEVGKHCAIVFGLPDFRLRWMFRRDGQVEEVAVRGDIAISSGLAIRRAARDGLGLALLPD
WLVGPDLATGTLIDLFPDYDITATSFDTAAWLIYASRRYTPKKVRLMTKFLRERLGRPG
>Mature_299_residues
MDTAQLRIFVHVARRGSFAAAARDLDMDASAVSRAVAALEAELDARLMHRTTRAMSLTEAGQAYLARVEPILDELDRARD
EVASARAEPVGTLRLTASVAFGEACLMPLLAEMRTRFPRLKLDLILTDTNLDLATERIDMALRLAPDVRADVIGIRLFAT
RYRVVASPDYCAREGAPRTPQEVGKHCAIVFGLPDFRLRWMFRRDGQVEEVAVRGDIAISSGLAIRRAARDGLGLALLPD
WLVGPDLATGTLIDLFPDYDITATSFDTAAWLIYASRRYTPKKVRLMTKFLRERLGRPG

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=34.0136054421769, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=32.6599326599327, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=28.1879194630872, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=30.952380952381, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1789639, Length=287, Percent_Identity=27.1777003484321, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787589, Length=241, Percent_Identity=33.195020746888, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI145693193, Length=289, Percent_Identity=27.681660899654, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1789173, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=269, Percent_Identity=26.3940520446097, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=121, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=24.2320819112628, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33155; Mature: 33155

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTAQLRIFVHVARRGSFAAAARDLDMDASAVSRAVAALEAELDARLMHRTTRAMSLTEA
CCCCEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAYLARVEPILDELDRARDEVASARAEPVGTLRLTASVAFGEACLMPLLAEMRTRFPRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCE
KLDLILTDTNLDLATERIDMALRLAPDVRADVIGIRLFATRYRVVASPDYCAREGAPRTP
EEEEEEEECCCHHHHHHHHHHEEECCCCHHHHHHEEEEEEEEEEEECCCHHHCCCCCCCH
QEVGKHCAIVFGLPDFRLRWMFRRDGQVEEVAVRGDIAISSGLAIRRAARDGLGLALLPD
HHHCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCEEECCCHHHHHHHHCCCCEEECCC
WLVGPDLATGTLIDLFPDYDITATSFDTAAWLIYASRRYTPKKVRLMTKFLRERLGRPG
HHCCCCCCCCCEEEECCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDTAQLRIFVHVARRGSFAAAARDLDMDASAVSRAVAALEAELDARLMHRTTRAMSLTEA
CCCCEEEEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQAYLARVEPILDELDRARDEVASARAEPVGTLRLTASVAFGEACLMPLLAEMRTRFPRL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCE
KLDLILTDTNLDLATERIDMALRLAPDVRADVIGIRLFATRYRVVASPDYCAREGAPRTP
EEEEEEEECCCHHHHHHHHHHEEECCCCHHHHHHEEEEEEEEEEEECCCHHHCCCCCCCH
QEVGKHCAIVFGLPDFRLRWMFRRDGQVEEVAVRGDIAISSGLAIRRAARDGLGLALLPD
HHHCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCEEECCCHHHHHHHHCCCCEEECCC
WLVGPDLATGTLIDLFPDYDITATSFDTAAWLIYASRRYTPKKVRLMTKFLRERLGRPG
HHCCCCCCCCCEEEECCCCCCEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]