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The map label for this gene is gcvA [H]

Identifier: 154244901

GI number: 154244901

Start: 1070789

End: 1071658

Strand: Direct

Name: gcvA [H]

Synonym: Xaut_0951

Alternate gene names: 154244901

Gene position: 1070789-1071658 (Clockwise)

Preceding gene: 154244899

Following gene: 154244905

Centisome position: 20.17

GC content: 70.92

Gene sequence:

>870_bases
ATGTCCTTCAGCCTGCCTTCGCTGCGCGGCCTTGCCGCCCTTGATGCCCTGGCCCGCCACAGGCGGCAGGGGGCCGCGGC
GGAGGCCCTCGGCATCTCGCGGTCCGCGCTTTCCCACCGCATTGCCGACCTCGAGGCGGAACTCGGGGCCCGGCTGATCC
GCACCGAGGGCCGGGTCTCGGTGCTGACCGATGACGGCACCGCCCTCCTGGCTGCCATGGGCGACGCGATCGAGCGGATC
GAGGCGGCGGTCGCGCCGTTGCAGCGGCGGTGCCGGCAGATCCGGCTCTCCACGGTGAACACCCTCGCCGCCAACTGGCT
GCTGCCGCGCCTGCCGGAATTCCAGCGCGCCCATCCCGGCATCGAGATCGCCGTGCTCACGACGCAGAGGGTGGTGGACC
TCGTTGAGGAAGATATCGACTGCGCCATCCGCAACGGGTCGGGCGCCTGGCCGGATGTCGCGGCGACGCTGCTGTTCCGC
GAGACGCTGGTGCCCGTTGCGGCGCCGGGCCTTGTGCTCGGGCCTGCGGCCTCGTGGCCGGTGATCCGCGCCCGCGCGCG
ATATCGCGACTGGAGGCTGTGGTGGCAGGGGACGGGACAAGGCGGGGCGGTGCCAGGGAGCCGCATTGTGGTCGAGAACA
GGGCGCAAGCCCTCGAAGCGGCCTTGGCTGGCGGGGGCGTCTTGCTCACCGATGCGCGCTACATCGGGGCTCATGTGGCA
TCCGGACGCCTGCACGTGTTGGGACCGGCGGTGGAACTGGACACCGGAAACTATTTCATCCGCCGCAAGGGGGCACGCAA
CCCGCGGCACATGGATGCGCTCGAGCACTGGCTGGTGGAGCAGGGGAGTGCCGCAGGACAGGTGGAATGA

Upstream 100 bases:

>100_bases
AACGTCAAGGGCTGGAAACTAGCGGATGTCTGCGCTCGAATGAGTGAGAAGTTCTCACCTGTTGCCGGTCGCCGGCCCGG
TCGCGCTGAGCGGAGCCGCC

Downstream 100 bases:

>100_bases
CAGGCGGTGCCGCCGGTCGCATTCGCGCCGCAGCCGGCCAGCATCGGATCATTGACGGGAATTGCCCACCTTGGCCGACA
TGGTCTTGACCGCGCGGTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSFSLPSLRGLAALDALARHRRQGAAAEALGISRSALSHRIADLEAELGARLIRTEGRVSVLTDDGTALLAAMGDAIERI
EAAVAPLQRRCRQIRLSTVNTLAANWLLPRLPEFQRAHPGIEIAVLTTQRVVDLVEEDIDCAIRNGSGAWPDVAATLLFR
ETLVPVAAPGLVLGPAASWPVIRARARYRDWRLWWQGTGQGGAVPGSRIVVENRAQALEAALAGGGVLLTDARYIGAHVA
SGRLHVLGPAVELDTGNYFIRRKGARNPRHMDALEHWLVEQGSAAGQVE

Sequences:

>Translated_289_residues
MSFSLPSLRGLAALDALARHRRQGAAAEALGISRSALSHRIADLEAELGARLIRTEGRVSVLTDDGTALLAAMGDAIERI
EAAVAPLQRRCRQIRLSTVNTLAANWLLPRLPEFQRAHPGIEIAVLTTQRVVDLVEEDIDCAIRNGSGAWPDVAATLLFR
ETLVPVAAPGLVLGPAASWPVIRARARYRDWRLWWQGTGQGGAVPGSRIVVENRAQALEAALAGGGVLLTDARYIGAHVA
SGRLHVLGPAVELDTGNYFIRRKGARNPRHMDALEHWLVEQGSAAGQVE
>Mature_288_residues
SFSLPSLRGLAALDALARHRRQGAAAEALGISRSALSHRIADLEAELGARLIRTEGRVSVLTDDGTALLAAMGDAIERIE
AAVAPLQRRCRQIRLSTVNTLAANWLLPRLPEFQRAHPGIEIAVLTTQRVVDLVEEDIDCAIRNGSGAWPDVAATLLFRE
TLVPVAAPGLVLGPAASWPVIRARARYRDWRLWWQGTGQGGAVPGSRIVVENRAQALEAALAGGGVLLTDARYIGAHVAS
GRLHVLGPAVELDTGNYFIRRKGARNPRHMDALEHWLVEQGSAAGQVE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=31.496062992126, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=28.0821917808219, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=26.2237762237762, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693193, Length=163, Percent_Identity=29.4478527607362, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=257, Percent_Identity=24.5136186770428, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787589, Length=149, Percent_Identity=32.8859060402685, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31071; Mature: 30940

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSLPSLRGLAALDALARHRRQGAAAEALGISRSALSHRIADLEAELGARLIRTEGRVS
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEECCCCEE
VLTDDGTALLAAMGDAIERIEAAVAPLQRRCRQIRLSTVNTLAANWLLPRLPEFQRAHPG
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
IEIAVLTTQRVVDLVEEDIDCAIRNGSGAWPDVAATLLFRETLVPVAAPGLVLGPAASWP
EEEEEECHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCH
VIRARARYRDWRLWWQGTGQGGAVPGSRIVVENRAQALEAALAGGGVLLTDARYIGAHVA
HHHHHHHCCCEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEECCHHHHHEEC
SGRLHVLGPAVELDTGNYFIRRKGARNPRHMDALEHWLVEQGSAAGQVE
CCEEEEECCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SFSLPSLRGLAALDALARHRRQGAAAEALGISRSALSHRIADLEAELGARLIRTEGRVS
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEECCCCEE
VLTDDGTALLAAMGDAIERIEAAVAPLQRRCRQIRLSTVNTLAANWLLPRLPEFQRAHPG
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
IEIAVLTTQRVVDLVEEDIDCAIRNGSGAWPDVAATLLFRETLVPVAAPGLVLGPAASWP
EEEEEECHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCH
VIRARARYRDWRLWWQGTGQGGAVPGSRIVVENRAQALEAALAGGGVLLTDARYIGAHVA
HHHHHHHCCCEEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHCCCCEEEECCHHHHHEEC
SGRLHVLGPAVELDTGNYFIRRKGARNPRHMDALEHWLVEQGSAAGQVE
CCEEEEECCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]