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The map label for this gene is cls [H]

Identifier: 154244896

GI number: 154244896

Start: 1066568

End: 1068013

Strand: Direct

Name: cls [H]

Synonym: Xaut_0946

Alternate gene names: 154244896

Gene position: 1066568-1068013 (Clockwise)

Preceding gene: 154244891

Following gene: 154244897

Centisome position: 20.09

GC content: 68.53

Gene sequence:

>1446_bases
ATGCATGATTCCGCCGTCCTCGCCTGGATGCTGTTTGCGGCCCACGCGAGCCTCCAGCTTGTCTTCATCGTCCGCGCATT
GCTGCGCCCCCACCGGGAGCCCTCTTCGCGGTTTGCCTGGGTGCTGGTGATCCTGCTCGCGCCCGTGGTCGGCGTCCTGG
CCTATATCCTGTTCGGCGAGGTCAATTTGGGGCGCAAGCCCATCGAGCGGCTGCGCGCGGCGCTGAAGGCCCTGCCGCCC
GCCCCGGACGTGGCCGGAGCGTCGGCCGAGGCGATGGTCCCCGAACGCTGCGTGCCGCTGTTCCGGGTCGGCTATTCGGT
GAACCACTTCGTGCCGGTGGCCGGCAACCGGGCGGTGCTGCTGCCCGATTCCGCGGCCGCCATCGCCGCCATGGTCGCGG
ACATCGATGCCGCTCAACACAGCGTGCATGTGCTGTTCTATATCTGGCTCGCCGACGGGAGCGGCCTGAAGGTGATGGAG
GCGCTGAAGCGCGCCGCCGCGCGCGGCGTCGTCTGCCGGGCCATGGCCGATGATCTCGGCTCCCGGGCGCTGATCCGCTC
CGCCCATTGGCGCGACATGGCGACGAGCGGGGTGAGGCTGGCGGCGGCGCTGCCGGTGGGCAATCCCCTGCTGCGGCTGT
TCAAGGGCCGGATCGACATGCGCAATCACCGCAAGATCGTGGTGATCGACAATGCCGTCACCTATTGCGGCAGCCAGAAT
TGCGCCGACGCCGCCTTCGCGGTGAAGGCCCGGTTCGCGCCCTGGGTCGATGTGATGGCCCGCTTTGAGGGGCCGGTGGT
GCGGCAGAACCAGTATCTGTTCGCCAGCAACTGGATGGCGCAGACCGACGAGGATCTTGCGTCCCTGTTCGCCGAGCCGC
TGCCTGCCCAGGTCCCGGGCTTCGTCGCGCAGGTGATCGGCTCGGGCGCCGGCGTGCGCTATTCGGCCATGCCGGAGACG
TTCGTCGCGCTGATGAATGCGGCCCGGCGCGAACTGGTGATCACTACGCCCTATTACGTCCCGGATGAGCCGCTCCAGGC
CGCTCTGTGCGCCAGCGCCCGGCGTGGGGTCGCGACCACCATCGTCTTTCCCGGGCGCAACGATTCCTGGATCGTGGCGG
CCGCCAGCCGCAGCTATTATCGCGACCTGCTGGAGGCGGGCGTGGAAATCCGCGAATATGTCGGCGGCCTGCTGCACGCC
AAGACGCTCACCCTCGACGGCGAGGTGACCCTGATCGGCTCCGCCAATATCGATCGCCGCAGCTTCGAGCTCAACGCGGA
GAACAACATCCTCCTGCACGACGAAGCCTTCACCCGCGCGGTCCGGGCCCGCCAGCAGGTGTTCCTCGATGCCGCAACGC
CGGTGACGCGGGCGCAAGTGGACGCCTACAGCGTGGGGCGGCGGCTCTGGAACAATGCCATCGCCATGCTCGGGCCGGTC
CTGTAG

Upstream 100 bases:

>100_bases
TCATCCGGCCGCGTCGCAACGCGCCGCAGCGCGGGCGGCGTTCGCGCAGGCGCCGAACATGGTTTAATGCGGGCTGCTTC
GCTCAAGCGTGTGCCGCGAC

Downstream 100 bases:

>100_bases
GTGCGCGATGCCGGGGGGCCAAACCGTCGGATTGGTATTTGCGAACGGCGCACGCGCTGATGTAAATTAAGGCATGCTCA
CTTCCGCGCCACGCCCCAGC

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MHDSAVLAWMLFAAHASLQLVFIVRALLRPHREPSSRFAWVLVILLAPVVGVLAYILFGEVNLGRKPIERLRAALKALPP
APDVAGASAEAMVPERCVPLFRVGYSVNHFVPVAGNRAVLLPDSAAAIAAMVADIDAAQHSVHVLFYIWLADGSGLKVME
ALKRAAARGVVCRAMADDLGSRALIRSAHWRDMATSGVRLAAALPVGNPLLRLFKGRIDMRNHRKIVVIDNAVTYCGSQN
CADAAFAVKARFAPWVDVMARFEGPVVRQNQYLFASNWMAQTDEDLASLFAEPLPAQVPGFVAQVIGSGAGVRYSAMPET
FVALMNAARRELVITTPYYVPDEPLQAALCASARRGVATTIVFPGRNDSWIVAAASRSYYRDLLEAGVEIREYVGGLLHA
KTLTLDGEVTLIGSANIDRRSFELNAENNILLHDEAFTRAVRARQQVFLDAATPVTRAQVDAYSVGRRLWNNAIAMLGPV
L

Sequences:

>Translated_481_residues
MHDSAVLAWMLFAAHASLQLVFIVRALLRPHREPSSRFAWVLVILLAPVVGVLAYILFGEVNLGRKPIERLRAALKALPP
APDVAGASAEAMVPERCVPLFRVGYSVNHFVPVAGNRAVLLPDSAAAIAAMVADIDAAQHSVHVLFYIWLADGSGLKVME
ALKRAAARGVVCRAMADDLGSRALIRSAHWRDMATSGVRLAAALPVGNPLLRLFKGRIDMRNHRKIVVIDNAVTYCGSQN
CADAAFAVKARFAPWVDVMARFEGPVVRQNQYLFASNWMAQTDEDLASLFAEPLPAQVPGFVAQVIGSGAGVRYSAMPET
FVALMNAARRELVITTPYYVPDEPLQAALCASARRGVATTIVFPGRNDSWIVAAASRSYYRDLLEAGVEIREYVGGLLHA
KTLTLDGEVTLIGSANIDRRSFELNAENNILLHDEAFTRAVRARQQVFLDAATPVTRAQVDAYSVGRRLWNNAIAMLGPV
L
>Mature_481_residues
MHDSAVLAWMLFAAHASLQLVFIVRALLRPHREPSSRFAWVLVILLAPVVGVLAYILFGEVNLGRKPIERLRAALKALPP
APDVAGASAEAMVPERCVPLFRVGYSVNHFVPVAGNRAVLLPDSAAAIAAMVADIDAAQHSVHVLFYIWLADGSGLKVME
ALKRAAARGVVCRAMADDLGSRALIRSAHWRDMATSGVRLAAALPVGNPLLRLFKGRIDMRNHRKIVVIDNAVTYCGSQN
CADAAFAVKARFAPWVDVMARFEGPVVRQNQYLFASNWMAQTDEDLASLFAEPLPAQVPGFVAQVIGSGAGVRYSAMPET
FVALMNAARRELVITTPYYVPDEPLQAALCASARRGVATTIVFPGRNDSWIVAAASRSYYRDLLEAGVEIREYVGGLLHA
KTLTLDGEVTLIGSANIDRRSFELNAENNILLHDEAFTRAVRARQQVFLDAATPVTRAQVDAYSVGRRLWNNAIAMLGPV
L

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=456, Percent_Identity=34.4298245614035, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1787007, Length=379, Percent_Identity=28.4960422163588, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081826, Length=397, Percent_Identity=27.455919395466, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52268; Mature: 52268

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDSAVLAWMLFAAHASLQLVFIVRALLRPHREPSSRFAWVLVILLAPVVGVLAYILFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VNLGRKPIERLRAALKALPPAPDVAGASAEAMVPERCVPLFRVGYSVNHFVPVAGNRAVL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCEEEECCCEEEE
LPDSAAAIAAMVADIDAAQHSVHVLFYIWLADGSGLKVMEALKRAAARGVVCRAMADDLG
ECCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCC
SRALIRSAHWRDMATSGVRLAAALPVGNPLLRLFKGRIDMRNHRKIVVIDNAVTYCGSQN
CHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCC
CADAAFAVKARFAPWVDVMARFEGPVVRQNQYLFASNWMAQTDEDLASLFAEPLPAQVPG
CCHHHHHHHHHCCHHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHCCCCCCCCHH
FVAQVIGSGAGVRYSAMPETFVALMNAARRELVITTPYYVPDEPLQAALCASARRGVATT
HHHHHHCCCCCCEECCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
IVFPGRNDSWIVAAASRSYYRDLLEAGVEIREYVGGLLHAKTLTLDGEVTLIGSANIDRR
EEECCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCC
SFELNAENNILLHDEAFTRAVRARQQVFLDAATPVTRAQVDAYSVGRRLWNNAIAMLGPV
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
L
C
>Mature Secondary Structure
MHDSAVLAWMLFAAHASLQLVFIVRALLRPHREPSSRFAWVLVILLAPVVGVLAYILFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
VNLGRKPIERLRAALKALPPAPDVAGASAEAMVPERCVPLFRVGYSVNHFVPVAGNRAVL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHCCCCCCCEEEECCCEEEE
LPDSAAAIAAMVADIDAAQHSVHVLFYIWLADGSGLKVMEALKRAAARGVVCRAMADDLG
ECCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCC
SRALIRSAHWRDMATSGVRLAAALPVGNPLLRLFKGRIDMRNHRKIVVIDNAVTYCGSQN
CHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCHHHCCCCC
CADAAFAVKARFAPWVDVMARFEGPVVRQNQYLFASNWMAQTDEDLASLFAEPLPAQVPG
CCHHHHHHHHHCCHHHHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHCCCCCCCCHH
FVAQVIGSGAGVRYSAMPETFVALMNAARRELVITTPYYVPDEPLQAALCASARRGVATT
HHHHHHCCCCCCEECCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
IVFPGRNDSWIVAAASRSYYRDLLEAGVEIREYVGGLLHAKTLTLDGEVTLIGSANIDRR
EEECCCCCCEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCC
SFELNAENNILLHDEAFTRAVRARQQVFLDAATPVTRAQVDAYSVGRRLWNNAIAMLGPV
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA