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The map label for this gene is puuA [H]

Identifier: 154244837

GI number: 154244837

Start: 990085

End: 991464

Strand: Direct

Name: puuA [H]

Synonym: Xaut_0887

Alternate gene names: 154244837

Gene position: 990085-991464 (Clockwise)

Preceding gene: 154244836

Following gene: 154244844

Centisome position: 18.65

GC content: 70.0

Gene sequence:

>1380_bases
ATGGGCACGCCTTCTCCCAGCGGCTCGGATCTCGATGAAGCCAAGGCCTTCCTTGCGCGCCATCCGGATCTGCAAGCCAT
CGACATCATCCTGACCGATTACCACGGCATCGGCCGCGGCAAGACCATCCGGCGCCATGAGCTGGAGGCGCTCTACAGCA
CCGGCCGGGGCATGCCCGCCTCCTTGTTCGGGCAGGATGTGGCCGGCGACGACGTGGAGGAGAGCGGCCTCATCCTCACC
GACGGCGGCGGCGATCAGGTCTGCTGGCCGATCCCCGGCACCCTCGGGCTCCTGCCCGCCACCGGGCGGGGCCAAGTGCT
GGTGAGCATGTACCACGGCGACGGCACGCCGCTTGGCGTCGAGCCGCGCCACGCGCTGGGCCGGCAGGTGGCCCGGGCCC
GCGCCGCCGGTTTCGAGCCCATGGGGGCGCTGGAGCTGGAATTCTATCTGGTGGACCGGGAGCGCGATGCGAGCGGGCGG
TACCAGCCGGCGCGCTATGCCCTCACCGGGCGGCGCTCGCTTGCCACCAACACCATGTCGGTGGACGAGCTGGACGAGAT
GTCTCCCCTGTTCGACGCCATCTACGCGGGCGCGGCGGAGCTGGGTGTGCCGCTGGAGTCACTCATCTCCGAGTACGCGC
CGGGGCAGTATGAGCTGACCATCCGCTATCGCGACCTGGCGCGGGCAACCGACGATGTGATCATCGCCAAGCGGCTGATC
CGCGCCACCGCGCGCCGCTTCGGCATGGAAGCCTGCTTCATGGCCAAGCCGTTCGGCGATCGCTCCGGCTCGGGCATGCA
CCTGCATCTGTCGCTGGCCGGGGCGGGCGGCGCCAATTTGTTCGCGGACCTGCCCTCGGGCGAGCTGAGCCCCCTCATGC
TCCACGCCATCGGCGGCATCCGCGCGACCATCGCCGACACCATGCTGGTGCTGGCGCCGTTCCTCAATTCCTGGCGGCGC
TTTGCCTCTGTGGTCTATTCCCCGGTCTCGGACACCTGGGGCGTGGAGAACCGGACGGTGGCAGTCCGCGTGCCTTCGGG
CAGCGCCAAGGGCCGCCATTTCGAGCACCGGGTGGCGGGGGTGGATGCCAACCCCTATCTGGTGGCGGCGGTGACGCTGG
CCGGCGCGCTCGACGGCATCGCGCAGCGCCGCGATCCCGGCCCGCCGGCGGAGGGCAATTCCTATGCGGGCGCGCGGGCG
GTCCATCTGCCGCGCAGCTGGCTGGACGCCATCGACTGCTTCGCGCAATCGGACTTCGCAAAGCAGGCGCTGGGGGCGGA
GCTACACCACAGCTTCGCCGCCATCAAGCGCGCGGAATGGCTGCGCCTCGCGCTGGAAGTGTCCGATGCCGAGTGGGCGC
TTTACGGCTTCACGGTCTGA

Upstream 100 bases:

>100_bases
CTGCTCGGACAGCGGGTTGCGTTTCCTTAAGCTAGAGTGCGATTCGCCGGGCAGGCGGATCCGATGGCCCGGAAAACGCG
CTCCAGCGGCAGGAACAGGA

Downstream 100 bases:

>100_bases
GCTTCGGCTTCGGCCTCAGGCACAGTCTTGCCCACCTGCCCGCGCCGCAGGAAGGCTTCGATCATGTCGGCGATGGCAGG
CGTGGAGGTTTCGCCCTCAA

Product: glutamine synthetase catalytic subunit

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MGTPSPSGSDLDEAKAFLARHPDLQAIDIILTDYHGIGRGKTIRRHELEALYSTGRGMPASLFGQDVAGDDVEESGLILT
DGGGDQVCWPIPGTLGLLPATGRGQVLVSMYHGDGTPLGVEPRHALGRQVARARAAGFEPMGALELEFYLVDRERDASGR
YQPARYALTGRRSLATNTMSVDELDEMSPLFDAIYAGAAELGVPLESLISEYAPGQYELTIRYRDLARATDDVIIAKRLI
RATARRFGMEACFMAKPFGDRSGSGMHLHLSLAGAGGANLFADLPSGELSPLMLHAIGGIRATIADTMLVLAPFLNSWRR
FASVVYSPVSDTWGVENRTVAVRVPSGSAKGRHFEHRVAGVDANPYLVAAVTLAGALDGIAQRRDPGPPAEGNSYAGARA
VHLPRSWLDAIDCFAQSDFAKQALGAELHHSFAAIKRAEWLRLALEVSDAEWALYGFTV

Sequences:

>Translated_459_residues
MGTPSPSGSDLDEAKAFLARHPDLQAIDIILTDYHGIGRGKTIRRHELEALYSTGRGMPASLFGQDVAGDDVEESGLILT
DGGGDQVCWPIPGTLGLLPATGRGQVLVSMYHGDGTPLGVEPRHALGRQVARARAAGFEPMGALELEFYLVDRERDASGR
YQPARYALTGRRSLATNTMSVDELDEMSPLFDAIYAGAAELGVPLESLISEYAPGQYELTIRYRDLARATDDVIIAKRLI
RATARRFGMEACFMAKPFGDRSGSGMHLHLSLAGAGGANLFADLPSGELSPLMLHAIGGIRATIADTMLVLAPFLNSWRR
FASVVYSPVSDTWGVENRTVAVRVPSGSAKGRHFEHRVAGVDANPYLVAAVTLAGALDGIAQRRDPGPPAEGNSYAGARA
VHLPRSWLDAIDCFAQSDFAKQALGAELHHSFAAIKRAEWLRLALEVSDAEWALYGFTV
>Mature_458_residues
GTPSPSGSDLDEAKAFLARHPDLQAIDIILTDYHGIGRGKTIRRHELEALYSTGRGMPASLFGQDVAGDDVEESGLILTD
GGGDQVCWPIPGTLGLLPATGRGQVLVSMYHGDGTPLGVEPRHALGRQVARARAAGFEPMGALELEFYLVDRERDASGRY
QPARYALTGRRSLATNTMSVDELDEMSPLFDAIYAGAAELGVPLESLISEYAPGQYELTIRYRDLARATDDVIIAKRLIR
ATARRFGMEACFMAKPFGDRSGSGMHLHLSLAGAGGANLFADLPSGELSPLMLHAIGGIRATIADTMLVLAPFLNSWRRF
ASVVYSPVSDTWGVENRTVAVRVPSGSAKGRHFEHRVAGVDANPYLVAAVTLAGALDGIAQRRDPGPPAEGNSYAGARAV
HLPRSWLDAIDCFAQSDFAKQALGAELHHSFAAIKRAEWLRLALEVSDAEWALYGFTV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=446, Percent_Identity=23.9910313901345, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI87081870, Length=447, Percent_Identity=37.8076062639821, Blast_Score=292, Evalue=4e-80,
Organism=Escherichia coli, GI1790301, Length=267, Percent_Identity=31.8352059925094, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49267; Mature: 49136

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTPSPSGSDLDEAKAFLARHPDLQAIDIILTDYHGIGRGKTIRRHELEALYSTGRGMPA
CCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCH
SLFGQDVAGDDVEESGLILTDGGGDQVCWPIPGTLGLLPATGRGQVLVSMYHGDGTPLGV
HHHCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCC
EPRHALGRQVARARAAGFEPMGALELEFYLVDRERDASGRYQPARYALTGRRSLATNTMS
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECCCCHHHCCCCC
VDELDEMSPLFDAIYAGAAELGVPLESLISEYAPGQYELTIRYRDLARATDDVIIAKRLI
HHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHCCCCEEEEEEEHHHHHCHHHHHHHHHHH
RATARRFGMEACFMAKPFGDRSGSGMHLHLSLAGAGGANLFADLPSGELSPLMLHAIGGI
HHHHHHHCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHCCH
RATIADTMLVLAPFLNSWRRFASVVYSPVSDTWGVENRTVAVRVPSGSAKGRHFEHRVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHEEEC
VDANPYLVAAVTLAGALDGIAQRRDPGPPAEGNSYAGARAVHLPRSWLDAIDCFAQSDFA
CCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
KQALGAELHHSFAAIKRAEWLRLALEVSDAEWALYGFTV
HHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEECC
>Mature Secondary Structure 
GTPSPSGSDLDEAKAFLARHPDLQAIDIILTDYHGIGRGKTIRRHELEALYSTGRGMPA
CCCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCH
SLFGQDVAGDDVEESGLILTDGGGDQVCWPIPGTLGLLPATGRGQVLVSMYHGDGTPLGV
HHHCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCC
EPRHALGRQVARARAAGFEPMGALELEFYLVDRERDASGRYQPARYALTGRRSLATNTMS
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECCCCHHHCCCCC
VDELDEMSPLFDAIYAGAAELGVPLESLISEYAPGQYELTIRYRDLARATDDVIIAKRLI
HHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHCCCCEEEEEEEHHHHHCHHHHHHHHHHH
RATARRFGMEACFMAKPFGDRSGSGMHLHLSLAGAGGANLFADLPSGELSPLMLHAIGGI
HHHHHHHCCCCEEEECCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHCCH
RATIADTMLVLAPFLNSWRRFASVVYSPVSDTWGVENRTVAVRVPSGSAKGRHFEHRVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHEEEC
VDANPYLVAAVTLAGALDGIAQRRDPGPPAEGNSYAGARAVHLPRSWLDAIDCFAQSDFA
CCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHH
KQALGAELHHSFAAIKRAEWLRLALEVSDAEWALYGFTV
HHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]