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The map label for this gene is rlmE

Identifier: 154244787

GI number: 154244787

Start: 935362

End: 936126

Strand: Direct

Name: rlmE

Synonym: Xaut_0837

Alternate gene names: 154244787

Gene position: 935362-936126 (Clockwise)

Preceding gene: 154244786

Following gene: 154244789

Centisome position: 17.62

GC content: 69.8

Gene sequence:

>765_bases
GTGACCACCCCACCCCGCGGGCCGGACGGCCGGCCCCTGAAAGTGCGCGTGAAAAAATCGCGCGGCCGCACCACGTCCTC
CCAGAAATGGCTGCAGCGCCAGCTCAACGACCCTTACGTCGCCCGCGCCAAGCGCGAGGGCTGGCGCTCGCGCGCCGCGT
TCAAGCTCATCGAGATGGACGAGAAGGCCCGGCTTTTGAAGCGCGGCATGCGCATCGTGGACCTGGGCGCTGCCCCCGGG
GGCTGGAGCCAGGTGGCGGCGAAGAAGATCGGCCTTGAGGAAGGCCTCGGCAAGATCGTCGCCATCGACCTTCTGGAGAT
CGAGCCCATTCCCGGTGTTGCCTTCGCCCAGATGGATTTCCTGGCGCCGGACGCACCGGAGCGGCTGATCGCCATGCTGG
GCGGGCAGGCCGACCTCGTCATGTCCGACATGGCGGCCAACGCGACCGGGCACAAGAAGACCGACCACCTGCGCATCGTC
GGCCTGGTGGAGCTGGCGGTGGAGTTCGCCCGACAGGTGCTGGCGCCGGGCGGGACCTTCCTCGCCAAGGTGATCCAGGG
CGGCATGGAAGCGACCCTTCTGGCCGACCTCAAGCGCGACTTCACCCAGGTGCGCCATGTGAAGCCGGCCGCCAGCCGTG
CCGATTCGGCCGAGCTGTATGTGCTGGCCACCGGCTTCCGCGGCGAGGCGGACCGGGCCGAGACGGACGATGCCGGGACC
GATGGCACCGGGACCGCCGAGGCGCAGGCGCCCCGCGATCAGTGA

Upstream 100 bases:

>100_bases
ACCGGGGCTTGCGCGAGGGCATGCTGGTGCAGTTGATGCGGGCTGATGGCGCCTGGCGGCGCAATCGCCCCGCCGGCACC
CCTTCCGGAGACGCACACGC

Downstream 100 bases:

>100_bases
TCGGGACTGACCTCGCTGCGCGGGTCGGCGAGGTCCGGCTTGCGGCCGTTCAGCTCCGCACCGGCATTCCGGGGCAGGCC
GACGAAATAGGCGAGGCTCG

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTTPPRGPDGRPLKVRVKKSRGRTTSSQKWLQRQLNDPYVARAKREGWRSRAAFKLIEMDEKARLLKRGMRIVDLGAAPG
GWSQVAAKKIGLEEGLGKIVAIDLLEIEPIPGVAFAQMDFLAPDAPERLIAMLGGQADLVMSDMAANATGHKKTDHLRIV
GLVELAVEFARQVLAPGGTFLAKVIQGGMEATLLADLKRDFTQVRHVKPAASRADSAELYVLATGFRGEADRAETDDAGT
DGTGTAEAQAPRDQ

Sequences:

>Translated_254_residues
MTTPPRGPDGRPLKVRVKKSRGRTTSSQKWLQRQLNDPYVARAKREGWRSRAAFKLIEMDEKARLLKRGMRIVDLGAAPG
GWSQVAAKKIGLEEGLGKIVAIDLLEIEPIPGVAFAQMDFLAPDAPERLIAMLGGQADLVMSDMAANATGHKKTDHLRIV
GLVELAVEFARQVLAPGGTFLAKVIQGGMEATLLADLKRDFTQVRHVKPAASRADSAELYVLATGFRGEADRAETDDAGT
DGTGTAEAQAPRDQ
>Mature_253_residues
TTPPRGPDGRPLKVRVKKSRGRTTSSQKWLQRQLNDPYVARAKREGWRSRAAFKLIEMDEKARLLKRGMRIVDLGAAPGG
WSQVAAKKIGLEEGLGKIVAIDLLEIEPIPGVAFAQMDFLAPDAPERLIAMLGGQADLVMSDMAANATGHKKTDHLRIVG
LVELAVEFARQVLAPGGTFLAKVIQGGMEATLLADLKRDFTQVRHVKPAASRADSAELYVLATGFRGEADRAETDDAGTD
GTGTAEAQAPRDQ

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=40.5529953917051, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI29029591, Length=206, Percent_Identity=37.8640776699029, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI29029589, Length=206, Percent_Identity=37.8640776699029, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI7110661, Length=206, Percent_Identity=37.8640776699029, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194097365, Length=191, Percent_Identity=36.1256544502618, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1789569, Length=206, Percent_Identity=47.0873786407767, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=38.974358974359, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553474, Length=219, Percent_Identity=36.0730593607306, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71987550, Length=196, Percent_Identity=34.6938775510204, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=196, Percent_Identity=34.6938775510204, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=196, Percent_Identity=34.6938775510204, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=212, Percent_Identity=33.4905660377358, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6319796, Length=217, Percent_Identity=31.3364055299539, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321302, Length=105, Percent_Identity=44.7619047619048, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356387, Length=229, Percent_Identity=39.7379912663755, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=37.3737373737374, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648639, Length=211, Percent_Identity=36.9668246445498, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI18859957, Length=191, Percent_Identity=35.0785340314136, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_XANP2 (A7IDJ5)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001415745.1
- ProteinModelPortal:   A7IDJ5
- SMR:   A7IDJ5
- STRING:   A7IDJ5
- GeneID:   5422139
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_0837
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK979300
- BioCyc:   XAUT78245:XAUT_0837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 27434; Mature: 27303

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: ACT_SITE 183-183 BINDING 80-80 BINDING 82-82 BINDING 103-103 BINDING 119-119 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPPRGPDGRPLKVRVKKSRGRTTSSQKWLQRQLNDPYVARAKREGWRSRAAFKLIEMD
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
EKARLLKRGMRIVDLGAAPGGWSQVAAKKIGLEEGLGKIVAIDLLEIEPIPGVAFAQMDF
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHCHHHCCCCEEEEEEEEECCCCCCHHHHHHH
LAPDAPERLIAMLGGQADLVMSDMAANATGHKKTDHLRIVGLVELAVEFARQVLAPGGTF
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHH
LAKVIQGGMEATLLADLKRDFTQVRHVKPAASRADSAELYVLATGFRGEADRAETDDAGT
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
DGTGTAEAQAPRDQ
CCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPPRGPDGRPLKVRVKKSRGRTTSSQKWLQRQLNDPYVARAKREGWRSRAAFKLIEMD
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
EKARLLKRGMRIVDLGAAPGGWSQVAAKKIGLEEGLGKIVAIDLLEIEPIPGVAFAQMDF
HHHHHHHCCCEEEEECCCCCCHHHHHHHHHCHHHCCCCEEEEEEEEECCCCCCHHHHHHH
LAPDAPERLIAMLGGQADLVMSDMAANATGHKKTDHLRIVGLVELAVEFARQVLAPGGTF
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHH
LAKVIQGGMEATLLADLKRDFTQVRHVKPAASRADSAELYVLATGFRGEADRAETDDAGT
HHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCC
DGTGTAEAQAPRDQ
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA