Click here to switch to the map view.

The map label for this gene is nifF [H]

Identifier: 154244782

GI number: 154244782

Start: 931556

End: 932038

Strand: Direct

Name: nifF [H]

Synonym: Xaut_0832

Alternate gene names: 154244782

Gene position: 931556-932038 (Clockwise)

Preceding gene: 154244781

Following gene: 154244783

Centisome position: 17.55

GC content: 61.49

Gene sequence:

>483_bases
ATGATTGTCACGGTGATCTTCGGCTCCGATGGCGGAGCCACGAAAGGGGTCGCGTCCAAGATTTCAAAGAAATGCCAGGG
CCGTTCGGTGGACATCAAAGCCGCGACCAAGGCCGATTTCGAGAATAGCGATCTTCTCATCCTCGGCTCGCCCACCTATG
GCGACGGAACATTGCAGACCGACTGGGAAGAGAACATCGACAAGCTGCGCAGCGCCAACCTGAAGGGCCGGAAGGTGGCG
CTGTTCGGCACGGGGGACCAGGAGAGCTATCCCACCTCCTTTGTCGATGCCATCGGCATCCTCTATGACGAGGTGACGGA
TCTCGGCGCGGTGGTGGTCGGCTTCACCGACACCGCCGGCTACGACTATGTGGGCTCCACGGCCGAACGGGACGGCCAGT
TCGTCGGCCTCGCCCTCGACCTCGACACGCAATCCGGTCTGACCGACAAGCGGGTGACGGCATGGCTCAGCAAGCTGCTT
TAG

Upstream 100 bases:

>100_bases
GAGCTAAATTCGTGTGGCTAGCATTTGCTTGCAAATTGGGATGCCGGCGTTGGCTGGTATTCCGTTCTAGATTTGAATCA
CTTCATAAAGAGACGGGTCC

Downstream 100 bases:

>100_bases
GCCCCTGCATGGACGTGCCGGAGGATGTGTCCCTCGCGCAGGAGGCCGGCTGGCCTCCGTCCCCGGCCGTCGCTGTTGCA
GAGGGGTGCGCGGCGCCGGT

Product: flavodoxin FldA

Products: NA

Alternate protein names: FldB [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MIVTVIFGSDGGATKGVASKISKKCQGRSVDIKAATKADFENSDLLILGSPTYGDGTLQTDWEENIDKLRSANLKGRKVA
LFGTGDQESYPTSFVDAIGILYDEVTDLGAVVVGFTDTAGYDYVGSTAERDGQFVGLALDLDTQSGLTDKRVTAWLSKLL

Sequences:

>Translated_160_residues
MIVTVIFGSDGGATKGVASKISKKCQGRSVDIKAATKADFENSDLLILGSPTYGDGTLQTDWEENIDKLRSANLKGRKVA
LFGTGDQESYPTSFVDAIGILYDEVTDLGAVVVGFTDTAGYDYVGSTAERDGQFVGLALDLDTQSGLTDKRVTAWLSKLL
>Mature_160_residues
MIVTVIFGSDGGATKGVASKISKKCQGRSVDIKAATKADFENSDLLILGSPTYGDGTLQTDWEENIDKLRSANLKGRKVA
LFGTGDQESYPTSFVDAIGILYDEVTDLGAVVVGFTDTAGYDYVGSTAERDGQFVGLALDLDTQSGLTDKRVTAWLSKLL

Specific function: Low-potential electron donor to a number of redox enzymes. NifF is the electron donor to nitrogenase [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=163, Percent_Identity=41.1042944785276, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI1789262, Length=165, Percent_Identity=38.1818181818182, Blast_Score=103, Evalue=4e-24,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001226
- InterPro:   IPR010086 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: NA

Molecular weight: Translated: 16997; Mature: 16997

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS00201 FLAVODOXIN ; PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTVIFGSDGGATKGVASKISKKCQGRSVDIKAATKADFENSDLLILGSPTYGDGTLQT
CEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
DWEENIDKLRSANLKGRKVALFGTGDQESYPTSFVDAIGILYDEVTDLGAVVVGFTDTAG
CHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCC
YDYVGSTAERDGQFVGLALDLDTQSGLTDKRVTAWLSKLL
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MIVTVIFGSDGGATKGVASKISKKCQGRSVDIKAATKADFENSDLLILGSPTYGDGTLQT
CEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
DWEENIDKLRSANLKGRKVALFGTGDQESYPTSFVDAIGILYDEVTDLGAVVVGFTDTAG
CHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCC
YDYVGSTAERDGQFVGLALDLDTQSGLTDKRVTAWLSKLL
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1859358 [H]