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The map label for this gene is ecaIM [H]

Identifier: 154244502

GI number: 154244502

Start: 613154

End: 615412

Strand: Direct

Name: ecaIM [H]

Synonym: Xaut_0547

Alternate gene names: 154244502

Gene position: 613154-615412 (Clockwise)

Preceding gene: 154244501

Following gene: 154244503

Centisome position: 11.55

GC content: 59.01

Gene sequence:

>2259_bases
GTGAGTCTGACTGACCGGGAGAAAGAACAGCTTAAGGCCATGATCGACGCGGGCCAGCCGCTGCCGCCACGATACAAGGC
CGTGCTGTTCGACCAGCCGCATGAAGCGGAGTTGATCTGGCCGGGAAAGACATCGGAAGTCACCAATGTCGTCCTCCCCT
TTCAGTCGATCGAGCAGATCGACGAGCCGCGAGCCGGCACGCAGGCCGGGACTACTGACTTGTTCGCCTTCGACCAGGCC
ACCGGGCGGCAGACGGGCGGCTGGGCGAACAAGCTTATCTGGGGCGACAATAAACTGGTCATCGCGTCGCTGAAGAACGG
ACCTCTGCGTCGCCAGATTGAAGACGCCGGCGGCCTGAAGCTGGTCTATATCGATCCGCCGTTCGACGTCGGCGCCGACT
TTTCGTTCGATATCGAGGTCGGAGATGAGAGTTTTACCAAACAGCCCAGCGTGATCGAAGAGGTCGCTTACCGCGACACA
TGGGGCGCCGGAACCCAATCATACGTTCATATGCTCTATGAACGTCTCTGTTTGATCCGCGACCTGCTGTCGCCGGACGG
CAGCCTCTATGTGCATGTCGGGTGGCAGGTGAGCGGGTACGTCCGGGTCATTCTCGACGAGATTTTCGGAAAGGGTGGCG
CGCCGGGCTTGCCCGGATTTCGTAATGAGATTGCTTGGAAATGCACAAGCGCCCACAGCGATAGCGGCCGCTATGGCATC
AATTGGCAAACCATCTTTTACTATACAAAGGGCGGCTCCTATACGCTCAACGAAACATATCAAGAGTACGATCAAGACTA
CGTTGACCAATATTATCGCTACAAAGACCCGAGCGGCCGCCGCTTCATGTCGGACAACTTGAATGCAGCGGGCTTGCAGG
GAGGTGGATACCCCTATGAATGGAAGGGCATAAAAAGAACTTGGCGCTGCCCTCCTGAGACAATGAAGCGGCTCGATGAA
GAAGGTAAGATATTCTACACGAAGAACGGCGTCCCGCGACTTAAGCGTTATTTGGACGAGGCCAAAGGCTTAAGCTGTCA
GACTCTCTGGGCCGACAAGGCCGTACAATACGTAGTCTCCTGGGGCCAAGAGAACACCGGGTATGAAACTCAGAAATCAG
AGTCATTACTGTCCAGAATCATCGATGCGTCATCCAAGCCGGGCGACTTGATCGCCGACTTCTTCTGCGGCTCGGGCACC
ACATTGGCCGTTGCCGAGAAGCTGGGCCGAAAATGGATCGGGGCGGATCTTGGCCGGTTCGCCATCCATACCAGCCGCAA
GCGCATGATCGGCGTGCAGCGCGGATTGCAGGAGGCAGGGAAGCCGTATCGCAGCTTCGAGATTCTGAACCTCGGGAAAT
ATGAGCGGCAGTATTTCGCGGGTATCGATCCCACGCTGCCGGAAGAGCAGCGCCGCGCGATCTCCCTGCAAAAGGAAGAG
CATTACCTCACGCTGATCCTCAGCGCCTACAAGGCCGAGCGCGTGTTCCAGTCGCCGCCTTTCCACGGCAAGAAGGCCGG
GGCGCTGGTACTCGTCGGGCCGATCGATGCGCCGGTCACGCAATCGCAGGTCCATGAGGCTGTTGAAGCCGCCCGCAAGC
TGCGCGTCAGCAAGCTCGACATTCTCGGCTTCGAGTTCGAGATGGGCATCGTGCCCCACGCGCAGGACGAAGCGCGGGCC
AAGGGCGTCAACGTCGCGCTTCGCTACATTCCCAAGGACGTGTTTGATCGCCGGGCCGTCGATAAGGGCCAGGTCGCCTT
CTACGATGTCGCCTATGTCGATGTGCAGCCGGTCGTGAAGGGCGCGTCCGTCACGGTGCGGCTGAAGGATTTTGGCGTCT
ATTATCGTCAGGACGATGTGGGCGCGTTGATCGAGTCGTTGAAGAATGGCGGCTCCAAGGTCACGGTCGATGCCGGGCAG
GTCGTGAAAATCACCAAGGACAAGAAGGGCGTGGTCAGCCGCGAGGTCCTGACCAAAGCCTGGAGCGACTGGGTCGATTA
CTGGGCCGTCGATTTCGACTTCGAGAACCGCAAGGAGATCGTGCGGATCACCGAGGCCGACGGCGCCGAGCGCGAAGCCT
GGACCGGGGGCTACATCTTCGAGAACGAATGGCAGTCATTCCGCACCCGGCAGGAGCGCACGCTGGAGCTGACGAGCGCG
CCGCACGACTACCCGAAGAAGGGCCGGTACAAGATCGGCGTCAAGGTCATCGATATTTTCGGCAATGACACGACCAAGGT
CGTCGAGGTTTCGCTGTGA

Upstream 100 bases:

>100_bases
CGACCCGTCGTCGTCGTCATGTCGGTAGAAGAGTACGAGCGCCTGACAGTCGCGGACTCCGCGCGCCAGGCACATGGGGA
ACCGGGCAAGGGGGTCGCCA

Downstream 100 bases:

>100_bases
AGGCCGGCCCTATCGAAATCGGGCGGCTCCTGCAAAACCGCCAGCGTTTCTGCGTTCCCATCTACCAGCGCCATTATGTC
TGGACGCGGCAGAAGCAATG

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 752; Mature: 751

Protein sequence:

>752_residues
MSLTDREKEQLKAMIDAGQPLPPRYKAVLFDQPHEAELIWPGKTSEVTNVVLPFQSIEQIDEPRAGTQAGTTDLFAFDQA
TGRQTGGWANKLIWGDNKLVIASLKNGPLRRQIEDAGGLKLVYIDPPFDVGADFSFDIEVGDESFTKQPSVIEEVAYRDT
WGAGTQSYVHMLYERLCLIRDLLSPDGSLYVHVGWQVSGYVRVILDEIFGKGGAPGLPGFRNEIAWKCTSAHSDSGRYGI
NWQTIFYYTKGGSYTLNETYQEYDQDYVDQYYRYKDPSGRRFMSDNLNAAGLQGGGYPYEWKGIKRTWRCPPETMKRLDE
EGKIFYTKNGVPRLKRYLDEAKGLSCQTLWADKAVQYVVSWGQENTGYETQKSESLLSRIIDASSKPGDLIADFFCGSGT
TLAVAEKLGRKWIGADLGRFAIHTSRKRMIGVQRGLQEAGKPYRSFEILNLGKYERQYFAGIDPTLPEEQRRAISLQKEE
HYLTLILSAYKAERVFQSPPFHGKKAGALVLVGPIDAPVTQSQVHEAVEAARKLRVSKLDILGFEFEMGIVPHAQDEARA
KGVNVALRYIPKDVFDRRAVDKGQVAFYDVAYVDVQPVVKGASVTVRLKDFGVYYRQDDVGALIESLKNGGSKVTVDAGQ
VVKITKDKKGVVSREVLTKAWSDWVDYWAVDFDFENRKEIVRITEADGAEREAWTGGYIFENEWQSFRTRQERTLELTSA
PHDYPKKGRYKIGVKVIDIFGNDTTKVVEVSL

Sequences:

>Translated_752_residues
MSLTDREKEQLKAMIDAGQPLPPRYKAVLFDQPHEAELIWPGKTSEVTNVVLPFQSIEQIDEPRAGTQAGTTDLFAFDQA
TGRQTGGWANKLIWGDNKLVIASLKNGPLRRQIEDAGGLKLVYIDPPFDVGADFSFDIEVGDESFTKQPSVIEEVAYRDT
WGAGTQSYVHMLYERLCLIRDLLSPDGSLYVHVGWQVSGYVRVILDEIFGKGGAPGLPGFRNEIAWKCTSAHSDSGRYGI
NWQTIFYYTKGGSYTLNETYQEYDQDYVDQYYRYKDPSGRRFMSDNLNAAGLQGGGYPYEWKGIKRTWRCPPETMKRLDE
EGKIFYTKNGVPRLKRYLDEAKGLSCQTLWADKAVQYVVSWGQENTGYETQKSESLLSRIIDASSKPGDLIADFFCGSGT
TLAVAEKLGRKWIGADLGRFAIHTSRKRMIGVQRGLQEAGKPYRSFEILNLGKYERQYFAGIDPTLPEEQRRAISLQKEE
HYLTLILSAYKAERVFQSPPFHGKKAGALVLVGPIDAPVTQSQVHEAVEAARKLRVSKLDILGFEFEMGIVPHAQDEARA
KGVNVALRYIPKDVFDRRAVDKGQVAFYDVAYVDVQPVVKGASVTVRLKDFGVYYRQDDVGALIESLKNGGSKVTVDAGQ
VVKITKDKKGVVSREVLTKAWSDWVDYWAVDFDFENRKEIVRITEADGAEREAWTGGYIFENEWQSFRTRQERTLELTSA
PHDYPKKGRYKIGVKVIDIFGNDTTKVVEVSL
>Mature_751_residues
SLTDREKEQLKAMIDAGQPLPPRYKAVLFDQPHEAELIWPGKTSEVTNVVLPFQSIEQIDEPRAGTQAGTTDLFAFDQAT
GRQTGGWANKLIWGDNKLVIASLKNGPLRRQIEDAGGLKLVYIDPPFDVGADFSFDIEVGDESFTKQPSVIEEVAYRDTW
GAGTQSYVHMLYERLCLIRDLLSPDGSLYVHVGWQVSGYVRVILDEIFGKGGAPGLPGFRNEIAWKCTSAHSDSGRYGIN
WQTIFYYTKGGSYTLNETYQEYDQDYVDQYYRYKDPSGRRFMSDNLNAAGLQGGGYPYEWKGIKRTWRCPPETMKRLDEE
GKIFYTKNGVPRLKRYLDEAKGLSCQTLWADKAVQYVVSWGQENTGYETQKSESLLSRIIDASSKPGDLIADFFCGSGTT
LAVAEKLGRKWIGADLGRFAIHTSRKRMIGVQRGLQEAGKPYRSFEILNLGKYERQYFAGIDPTLPEEQRRAISLQKEEH
YLTLILSAYKAERVFQSPPFHGKKAGALVLVGPIDAPVTQSQVHEAVEAARKLRVSKLDILGFEFEMGIVPHAQDEARAK
GVNVALRYIPKDVFDRRAVDKGQVAFYDVAYVDVQPVVKGASVTVRLKDFGVYYRQDDVGALIESLKNGGSKVTVDAGQV
VKITKDKKGVVSREVLTKAWSDWVDYWAVDFDFENRKEIVRITEADGAEREAWTGGYIFENEWQSFRTRQERTLELTSAP
HDYPKKGRYKIGVKVIDIFGNDTTKVVEVSL

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 84762; Mature: 84630

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00092 N6_MTASE ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTDREKEQLKAMIDAGQPLPPRYKAVLFDQPHEAELIWPGKTSEVTNVVLPFQSIEQI
CCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCCHHEEEECHHHHHHH
DEPRAGTQAGTTDLFAFDQATGRQTGGWANKLIWGDNKLVIASLKNGPLRRQIEDAGGLK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHCCCCEE
LVYIDPPFDVGADFSFDIEVGDESFTKQPSVIEEVAYRDTWGAGTQSYVHMLYERLCLIR
EEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DLLSPDGSLYVHVGWQVSGYVRVILDEIFGKGGAPGLPGFRNEIAWKCTSAHSDSGRYGI
HHHCCCCCEEEEECCEECCHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCCCCCCCC
NWQTIFYYTKGGSYTLNETYQEYDQDYVDQYYRYKDPSGRRFMSDNLNAAGLQGGGYPYE
EEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCEE
WKGIKRTWRCPPETMKRLDEEGKIFYTKNGVPRLKRYLDEAKGLSCQTLWADKAVQYVVS
ECCCCCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
WGQENTGYETQKSESLLSRIIDASSKPGDLIADFFCGSGTTLAVAEKLGRKWIGADLGRF
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEHHHHHHCHHHHCCCHHHH
AIHTSRKRMIGVQRGLQEAGKPYRSFEILNLGKYERQYFAGIDPTLPEEQRRAISLQKEE
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCCHHHHHHCCCCCCC
HYLTLILSAYKAERVFQSPPFHGKKAGALVLVGPIDAPVTQSQVHEAVEAARKLRVSKLD
CEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
ILGFEFEMGIVPHAQDEARAKGVNVALRYIPKDVFDRRAVDKGQVAFYDVAYVDVQPVVK
EEEEEEEECCCCCCCHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEEEEEEEEEHHHCCC
GASVTVRLKDFGVYYRQDDVGALIESLKNGGSKVTVDAGQVVKITKDKKGVVSREVLTKA
CCEEEEEEEECEEEEEECHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCHHHHHHHHHH
WSDWVDYWAVDFDFENRKEIVRITEADGAEREAWTGGYIFENEWQSFRTRQERTLELTSA
HHHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHEEEECCC
PHDYPKKGRYKIGVKVIDIFGNDTTKVVEVSL
CCCCCCCCCEEEEEEEEEEECCCCEEEEEEEC
>Mature Secondary Structure 
SLTDREKEQLKAMIDAGQPLPPRYKAVLFDQPHEAELIWPGKTSEVTNVVLPFQSIEQI
CCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCCHHEEEECHHHHHHH
DEPRAGTQAGTTDLFAFDQATGRQTGGWANKLIWGDNKLVIASLKNGPLRRQIEDAGGLK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCEEEEEECCCCCCHHHHHCCCCEE
LVYIDPPFDVGADFSFDIEVGDESFTKQPSVIEEVAYRDTWGAGTQSYVHMLYERLCLIR
EEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DLLSPDGSLYVHVGWQVSGYVRVILDEIFGKGGAPGLPGFRNEIAWKCTSAHSDSGRYGI
HHHCCCCCEEEEECCEECCHHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCCCCCCCC
NWQTIFYYTKGGSYTLNETYQEYDQDYVDQYYRYKDPSGRRFMSDNLNAAGLQGGGYPYE
EEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCEE
WKGIKRTWRCPPETMKRLDEEGKIFYTKNGVPRLKRYLDEAKGLSCQTLWADKAVQYVVS
ECCCCCCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
WGQENTGYETQKSESLLSRIIDASSKPGDLIADFFCGSGTTLAVAEKLGRKWIGADLGRF
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEEHHHHHHCHHHHCCCHHHH
AIHTSRKRMIGVQRGLQEAGKPYRSFEILNLGKYERQYFAGIDPTLPEEQRRAISLQKEE
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHCCCCCCCCHHHHHHCCCCCCC
HYLTLILSAYKAERVFQSPPFHGKKAGALVLVGPIDAPVTQSQVHEAVEAARKLRVSKLD
CEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
ILGFEFEMGIVPHAQDEARAKGVNVALRYIPKDVFDRRAVDKGQVAFYDVAYVDVQPVVK
EEEEEEEECCCCCCCHHHHHCCCEEEEECCCHHHHHHCCCCCCCEEEEEEEEEEHHHCCC
GASVTVRLKDFGVYYRQDDVGALIESLKNGGSKVTVDAGQVVKITKDKKGVVSREVLTKA
CCEEEEEEEECEEEEEECHHHHHHHHHHCCCCEEEEECCCEEEEECCCCCHHHHHHHHHH
WSDWVDYWAVDFDFENRKEIVRITEADGAEREAWTGGYIFENEWQSFRTRQERTLELTSA
HHHHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHHHEEEECCC
PHDYPKKGRYKIGVKVIDIFGNDTTKVVEVSL
CCCCCCCCCEEEEEEEEEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]