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The map label for this gene is bepF [H]

Identifier: 154244470

GI number: 154244470

Start: 570770

End: 572077

Strand: Direct

Name: bepF [H]

Synonym: Xaut_0513

Alternate gene names: 154244470

Gene position: 570770-572077 (Clockwise)

Preceding gene: 154244468

Following gene: 154244471

Centisome position: 10.75

GC content: 68.27

Gene sequence:

>1308_bases
ATGGTGGGGGGGATCGGGGTGTCCGGCGGGCTGCGCCACGGCATGGTGGCGCTTTGCGCGGCTTTTCTGCTCTCGGCCTG
TGAGGAAAAGAACACCTACGTACCGCCGCCCCCTGCCGCCGTCACCGTAGCCCCCGTTCTTGAGAAGCCGGTCACCCGCT
TCCTCGAGCTGACCGGCAACACCGCCGCCATCAATTCGGTGGACCTGGAAGCCCGCGTCCAGGGCTTCCTCACTCAGATC
AACTACAAGGACGGCCAGTTCGCCAAGAAGGGCACCGTCCTGTTTGTGATCGAGCAGGACCAGTACAAGGCCCAGGTGGA
CCAGGCCAAGGCCCAGCTCGCTGCCAACCAGGCATCGCAGGTGCAGGCCGAGGCCGAGTACAACCGCCAGGCCCAGCTCG
CCAAGCAGGACTTCGCCTCGCAGGCCACCCTCGACCAGGCCCGCGCCAAGCGCGATTCGGCTGTCGCCGAGGTGGCGAAC
GCCCAGGCCTCCCTGGTGCTGGCGCAGATCAATCTCGGCTACACCCAGGTCACTGCCCCCTTCGACGGCGCCGTCACCGC
CCATCTCCAGGACGTGGGAGCGCTGGTGGGTTACAGCGGACCCACCAAGCTCGCCACCGTGGTGCAGATCAACCCCATCT
GGGTCTGGTTCACCCTGAGCGAGCAGGAGGTGCTGCGGATCAAGGAGCACCTCGCCAGGCAGGGGCGCTCGCTGGTCTCC
CTGACCCGCGACGTGCCGGACCTTGCCATCGAGATCGGCCTCCAGACCGAGACCGGCTACCCCCACCAGGGCAAGATCGA
CTACGTGGCGCCGCAGGTGGACCCCAACCTCGGCACCCTGACCGTCCGCGGCATCTTCCAGAATGACGATTTCGCGCTGC
TGCCGGGCCTGTTCGCGCGGGTGAGGGTGCCGCTGGGCGCTGCTCAGACCACCGTGCTGGTGCCGGATTCCGCCATCAAT
TCCAACCAGCTTGGTCCCTACGTCCTGACCGTGAGCACGGACAACGTGGTCTCGCAGACCCAGGTGACCCTTGGCCAGCT
CCAGGCCGACGGGCTGCGCGCCATCCAGACCGGGCTGAAGGCCGGCGACCGCATCATCATCAGCGGCATCCAGCGCGCGG
TGCCGGGCTCCAAGGTGAAGGTGGAGGAGGGCACGATCGTCGGCATCGCGCCGCCCGCCGTCGACCAGGGCAACAAACCG
CTGCCCAAGCCCGCGGGCGTCGCGCCCGCCGATGCACCCGCCAAGGCCGCGCCCTCGGTGGCGCCGTCCGCGGCACCGTC
GCAGGCTCCCGCGACACAGAAGAACTGA

Upstream 100 bases:

>100_bases
GTCGTCGGGGCCGCTGGCGCCGTTCGGCCCATTCCTCTCATGGACACGTCATGACCCGGTGCTTAGTATCCGGCGCAACT
GCGATATGGGGGAATGCGGT

Downstream 100 bases:

>100_bases
CCCGCACTCGAAGAAAGAGGCAGCGCCCATGATATCGCGCTTTTTCATCGAGCGGCCGGTCCTCTCGAACGTACTCGCAC
TTGTCTTCGTGCTGATCGGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MVGGIGVSGGLRHGMVALCAAFLLSACEEKNTYVPPPPAAVTVAPVLEKPVTRFLELTGNTAAINSVDLEARVQGFLTQI
NYKDGQFAKKGTVLFVIEQDQYKAQVDQAKAQLAANQASQVQAEAEYNRQAQLAKQDFASQATLDQARAKRDSAVAEVAN
AQASLVLAQINLGYTQVTAPFDGAVTAHLQDVGALVGYSGPTKLATVVQINPIWVWFTLSEQEVLRIKEHLARQGRSLVS
LTRDVPDLAIEIGLQTETGYPHQGKIDYVAPQVDPNLGTLTVRGIFQNDDFALLPGLFARVRVPLGAAQTTVLVPDSAIN
SNQLGPYVLTVSTDNVVSQTQVTLGQLQADGLRAIQTGLKAGDRIIISGIQRAVPGSKVKVEEGTIVGIAPPAVDQGNKP
LPKPAGVAPADAPAKAAPSVAPSAAPSQAPATQKN

Sequences:

>Translated_435_residues
MVGGIGVSGGLRHGMVALCAAFLLSACEEKNTYVPPPPAAVTVAPVLEKPVTRFLELTGNTAAINSVDLEARVQGFLTQI
NYKDGQFAKKGTVLFVIEQDQYKAQVDQAKAQLAANQASQVQAEAEYNRQAQLAKQDFASQATLDQARAKRDSAVAEVAN
AQASLVLAQINLGYTQVTAPFDGAVTAHLQDVGALVGYSGPTKLATVVQINPIWVWFTLSEQEVLRIKEHLARQGRSLVS
LTRDVPDLAIEIGLQTETGYPHQGKIDYVAPQVDPNLGTLTVRGIFQNDDFALLPGLFARVRVPLGAAQTTVLVPDSAIN
SNQLGPYVLTVSTDNVVSQTQVTLGQLQADGLRAIQTGLKAGDRIIISGIQRAVPGSKVKVEEGTIVGIAPPAVDQGNKP
LPKPAGVAPADAPAKAAPSVAPSAAPSQAPATQKN
>Mature_435_residues
MVGGIGVSGGLRHGMVALCAAFLLSACEEKNTYVPPPPAAVTVAPVLEKPVTRFLELTGNTAAINSVDLEARVQGFLTQI
NYKDGQFAKKGTVLFVIEQDQYKAQVDQAKAQLAANQASQVQAEAEYNRQAQLAKQDFASQATLDQARAKRDSAVAEVAN
AQASLVLAQINLGYTQVTAPFDGAVTAHLQDVGALVGYSGPTKLATVVQINPIWVWFTLSEQEVLRIKEHLARQGRSLVS
LTRDVPDLAIEIGLQTETGYPHQGKIDYVAPQVDPNLGTLTVRGIFQNDDFALLPGLFARVRVPLGAAQTTVLVPDSAIN
SNQLGPYVLTVSTDNVVSQTQVTLGQLQADGLRAIQTGLKAGDRIIISGIQRAVPGSKVKVEEGTIVGIAPPAVDQGNKP
LPKPAGVAPADAPAKAAPSVAPSAAPSQAPATQKN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=368, Percent_Identity=31.25, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1786668, Length=384, Percent_Identity=33.8541666666667, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789665, Length=367, Percent_Identity=32.9700272479564, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87082036, Length=347, Percent_Identity=31.1239193083574, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87081796, Length=187, Percent_Identity=32.620320855615, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789637, Length=177, Percent_Identity=31.638418079096, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081951, Length=163, Percent_Identity=30.6748466257669, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45692; Mature: 45692

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGGIGVSGGLRHGMVALCAAFLLSACEEKNTYVPPPPAAVTVAPVLEKPVTRFLELTGN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCC
TAAINSVDLEARVQGFLTQINYKDGQFAKKGTVLFVIEQDQYKAQVDQAKAQLAANQASQ
EEEEECCCHHHHHHHHHHEECCCCCCEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
VQAEAEYNRQAQLAKQDFASQATLDQARAKRDSAVAEVANAQASLVLAQINLGYTQVTAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEECC
FDGAVTAHLQDVGALVGYSGPTKLATVVQINPIWVWFTLSEQEVLRIKEHLARQGRSLVS
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCHHHH
LTRDVPDLAIEIGLQTETGYPHQGKIDYVAPQVDPNLGTLTVRGIFQNDDFALLPGLFAR
HHHCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCEEHHHHHHHH
VRVPLGAAQTTVLVPDSAINSNQLGPYVLTVSTDNVVSQTQVTLGQLQADGLRAIQTGLK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
AGDRIIISGIQRAVPGSKVKVEEGTIVGIAPPAVDQGNKPLPKPAGVAPADAPAKAAPSV
CCCEEEEEHHHHCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APSAAPSQAPATQKN
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MVGGIGVSGGLRHGMVALCAAFLLSACEEKNTYVPPPPAAVTVAPVLEKPVTRFLELTGN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCC
TAAINSVDLEARVQGFLTQINYKDGQFAKKGTVLFVIEQDQYKAQVDQAKAQLAANQASQ
EEEEECCCHHHHHHHHHHEECCCCCCEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHH
VQAEAEYNRQAQLAKQDFASQATLDQARAKRDSAVAEVANAQASLVLAQINLGYTQVTAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEECC
FDGAVTAHLQDVGALVGYSGPTKLATVVQINPIWVWFTLSEQEVLRIKEHLARQGRSLVS
CCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCHHHH
LTRDVPDLAIEIGLQTETGYPHQGKIDYVAPQVDPNLGTLTVRGIFQNDDFALLPGLFAR
HHHCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCEEHHHHHHHH
VRVPLGAAQTTVLVPDSAINSNQLGPYVLTVSTDNVVSQTQVTLGQLQADGLRAIQTGLK
HCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
AGDRIIISGIQRAVPGSKVKVEEGTIVGIAPPAVDQGNKPLPKPAGVAPADAPAKAAPSV
CCCEEEEEHHHHCCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APSAAPSQAPATQKN
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]