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The map label for this gene is iclR [H]

Identifier: 154244438

GI number: 154244438

Start: 532763

End: 533617

Strand: Direct

Name: iclR [H]

Synonym: Xaut_0481

Alternate gene names: 154244438

Gene position: 532763-533617 (Clockwise)

Preceding gene: 154244435

Following gene: 154244439

Centisome position: 10.04

GC content: 71.58

Gene sequence:

>855_bases
ATGCCGGAGACCGAGGTCGCGCCACGCCGCAGGGGTCGCCCCGCCTCGGGCACCGCCGCCGAGGGTGGGGCCGAGGTCCA
GTCGCTGGATCGCGCCATCGCCCTGCTGCGGGTGGTGGCGGACCAGGATGGCATGGCGCTGGCCGAGATCGCCCGCCGGG
CGGAACTGCCGGTGTCCACCGTGCATCGGCTGCTCACCACGCTGGAGAAGCGCCAGCTGGTGAATCACGACCTGTCCACC
GGGCTGTGGACCGTGGGGCTCGGACTGTTCCGCATCGGCTCGGCCTACCTGCGCATCCGCAAGCTGCCGGACATCGGCCG
CCCGGTGATCCGCCGGCTGCTGCACGAGACCGACGAGACCGTGAACCTCGCCATGTTCGACGCCATGGAGCTGGTGGTGG
TGGCCCAGGCGGAGGCGCATGCCCCGGTGCGCGCCTTCTTCCGCCTCGGAGCGCGGCTGCCGCTGCATGCCACCGCCGCC
GGCAAGGCCGTGCTGGCCGCCGCCACGCCCGCCTTCCGCGGCGCCTGCCTTGGCCAGATCCGCTACGAGCCCTTCACCAC
CGCCACCCACCGCGAGGAGCGGGAGCTGATGGACGATGTGGCTGAGATTCTGGAACGTGGCTTCGCCGTGGACCGGGAAG
AGCATGCCCCCGGCATGCGAGCGGTGGCCGCAGCGATTCTCAACGAATGGCGCGACCCCATCGGCGCGGTCTCGGTCTCC
GCGCCCGCGGTGCGCATGAGCGAGGACCGCATCCTGCGCCTCGGCCGGCGCGTGGCCGCCGCCGCCGAGCAGATGACGCG
GCTTTATTCCGGCGTCGATGAAAGCGTGTCGCAGGATTACGCTGCGTATCGGTAA

Upstream 100 bases:

>100_bases
CGGAAGATGGAAAATTTGTCACAGGGTGGACGCCGGCCGCCGTCCAGCTACTTTGGGGGCCGACGCGCTGAGCCGCACCA
AGCAGGTAGGGAGTATTGGG

Downstream 100 bases:

>100_bases
GGCCTGTTGACTGAATTGGCATACAGTATTCCAAATATTGGAATACGGAGTACCAAATTCGGAATTGAAAATTCCAATTG
ACACAAGGGAAGCTCATTAT

Product: transcriptional regulator IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPETEVAPRRRGRPASGTAAEGGAEVQSLDRAIALLRVVADQDGMALAEIARRAELPVSTVHRLLTTLEKRQLVNHDLST
GLWTVGLGLFRIGSAYLRIRKLPDIGRPVIRRLLHETDETVNLAMFDAMELVVVAQAEAHAPVRAFFRLGARLPLHATAA
GKAVLAAATPAFRGACLGQIRYEPFTTATHREERELMDDVAEILERGFAVDREEHAPGMRAVAAAILNEWRDPIGAVSVS
APAVRMSEDRILRLGRRVAAAAEQMTRLYSGVDESVSQDYAAYR

Sequences:

>Translated_284_residues
MPETEVAPRRRGRPASGTAAEGGAEVQSLDRAIALLRVVADQDGMALAEIARRAELPVSTVHRLLTTLEKRQLVNHDLST
GLWTVGLGLFRIGSAYLRIRKLPDIGRPVIRRLLHETDETVNLAMFDAMELVVVAQAEAHAPVRAFFRLGARLPLHATAA
GKAVLAAATPAFRGACLGQIRYEPFTTATHREERELMDDVAEILERGFAVDREEHAPGMRAVAAAILNEWRDPIGAVSVS
APAVRMSEDRILRLGRRVAAAAEQMTRLYSGVDESVSQDYAAYR
>Mature_283_residues
PETEVAPRRRGRPASGTAAEGGAEVQSLDRAIALLRVVADQDGMALAEIARRAELPVSTVHRLLTTLEKRQLVNHDLSTG
LWTVGLGLFRIGSAYLRIRKLPDIGRPVIRRLLHETDETVNLAMFDAMELVVVAQAEAHAPVRAFFRLGARLPLHATAAG
KAVLAAATPAFRGACLGQIRYEPFTTATHREERELMDDVAEILERGFAVDREEHAPGMRAVAAAILNEWRDPIGAVSVSA
PAVRMSEDRILRLGRRVAAAAEQMTRLYSGVDESVSQDYAAYR

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=269, Percent_Identity=38.6617100371747, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1786715, Length=265, Percent_Identity=36.2264150943396, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=29.0322580645161, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788131, Length=226, Percent_Identity=26.1061946902655, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789999, Length=246, Percent_Identity=28.0487804878049, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=24.5967741935484, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31045; Mature: 30914

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETEVAPRRRGRPASGTAAEGGAEVQSLDRAIALLRVVADQDGMALAEIARRAELPVST
CCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
VHRLLTTLEKRQLVNHDLSTGLWTVGLGLFRIGSAYLRIRKLPDIGRPVIRRLLHETDET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
VNLAMFDAMELVVVAQAEAHAPVRAFFRLGARLPLHATAAGKAVLAAATPAFRGACLGQI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEHHCCCCHHHHCCHHHHHHHHHCE
RYEPFTTATHREERELMDDVAEILERGFAVDREEHAPGMRAVAAAILNEWRDPIGAVSVS
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEECC
APAVRMSEDRILRLGRRVAAAAEQMTRLYSGVDESVSQDYAAYR
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCC
>Mature Secondary Structure 
PETEVAPRRRGRPASGTAAEGGAEVQSLDRAIALLRVVADQDGMALAEIARRAELPVST
CCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
VHRLLTTLEKRQLVNHDLSTGLWTVGLGLFRIGSAYLRIRKLPDIGRPVIRRLLHETDET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCH
VNLAMFDAMELVVVAQAEAHAPVRAFFRLGARLPLHATAAGKAVLAAATPAFRGACLGQI
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEHHCCCCHHHHCCHHHHHHHHHCE
RYEPFTTATHREERELMDDVAEILERGFAVDREEHAPGMRAVAAAILNEWRDPIGAVSVS
ECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCEECC
APAVRMSEDRILRLGRRVAAAAEQMTRLYSGVDESVSQDYAAYR
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]