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The map label for this gene is mdh1 [H]

Identifier: 154244215

GI number: 154244215

Start: 262851

End: 263834

Strand: Reverse

Name: mdh1 [H]

Synonym: Xaut_0258

Alternate gene names: 154244215

Gene position: 263834-262851 (Counterclockwise)

Preceding gene: 154244216

Following gene: 154244208

Centisome position: 4.97

GC content: 61.79

Gene sequence:

>984_bases
ATGACCGGGCACGTAAAACAGATCGCCGTGACAGGAGCTGCGGGACAGATCTGTTATTCCCTGCTATTCCGCATTGCCAG
GGGAGAGCTCTACGGCCCGCGCCAGCCCATATCTCTGCGGCTGCTCGACCTTCCCCAGGTTCAGCCGGCGCTTCGCGGCG
TCGTGATGGAACTTGAGGACGGCGCGTTTCCCCTGCTTGCGGACGTCGCCGTGACCGACGATGCGAGACTTGCGTTCCGC
GACATCGATGCTGCGATTCTGGTGGGTTCGCGTCCGCGGTCCAAGGGGATGGAAAGGCGCGACCTGCTTGCCACCAACGC
CGAGATCTTCAGACTGCAAGGGCGGGCCCTGAACGACGTCGCCAAACTGGACGCGAAGGTGCTGGTGGTTGGCAATCCGG
CAAACACCAATGCAATGATCCTGAGCGAGCACGCGCCTGAATTTCCTTCCGAGAATATCACATCGATGATCCGCCTCGAT
CATAACCGCGCGCTGGCCCAGCTCGCGCGAAAAGCGCTGGTCGGGCTGGAGGAGATCAAAGGACTGGTCGTATGGGGCAA
TCACTCACCGACCATGTTCGTGGATTGGTCTCATGCGACAGTCGCCGGCCGACCGCTTGCCGACATCGTCGCTGACCATG
ACTGGTATCACAAGACACTGATCCCACAGGTCGCCCGCCGTGGGACGGCCGTCATCGAAGCGCGCGGCGCCTCCTCCGCG
GCGTCGGCGGCGAATGCCGCTATCGATCACATGCGCGACTGGATCAATGGCAGCGGCGGCGACTGGGTCTCGATGGGACT
ATGCTCGGACGGCTCTTACGGAATTCCCGAAGGTCTCTTTTGCGGCAGTCCAGCCATTTGCGCCGAGGGGCAATATGCCC
GCGTGGCAGACTTGAAGATCCGTGGATATGCTCGCGTGATGCTGGATCGAACCGTTGCAGAGCTGATTGAGGAACGCGAG
GCCGTCCGCGCCCTTGTGAGTTGA

Upstream 100 bases:

>100_bases
TGATGCCGGTGGCGCTCGCAATCGGCGGCACTGCCATCATCCTGTGCGCCGTTTGGGCGGGAATTTGAGACCAGACCTGA
CCATGGGAAGGAGTAAACTG

Downstream 100 bases:

>100_bases
GACTCTTCGCATTGGATCAAGGTGGATAGAGACGCCTCGGACTTTTTTCTCTTGTTGCGGCGCCGTCGAGCCAACAAGCC
CCACGCGGCGAGAGCATCAG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTGHVKQIAVTGAAGQICYSLLFRIARGELYGPRQPISLRLLDLPQVQPALRGVVMELEDGAFPLLADVAVTDDARLAFR
DIDAAILVGSRPRSKGMERRDLLATNAEIFRLQGRALNDVAKLDAKVLVVGNPANTNAMILSEHAPEFPSENITSMIRLD
HNRALAQLARKALVGLEEIKGLVVWGNHSPTMFVDWSHATVAGRPLADIVADHDWYHKTLIPQVARRGTAVIEARGASSA
ASAANAAIDHMRDWINGSGGDWVSMGLCSDGSYGIPEGLFCGSPAICAEGQYARVADLKIRGYARVMLDRTVAELIEERE
AVRALVS

Sequences:

>Translated_327_residues
MTGHVKQIAVTGAAGQICYSLLFRIARGELYGPRQPISLRLLDLPQVQPALRGVVMELEDGAFPLLADVAVTDDARLAFR
DIDAAILVGSRPRSKGMERRDLLATNAEIFRLQGRALNDVAKLDAKVLVVGNPANTNAMILSEHAPEFPSENITSMIRLD
HNRALAQLARKALVGLEEIKGLVVWGNHSPTMFVDWSHATVAGRPLADIVADHDWYHKTLIPQVARRGTAVIEARGASSA
ASAANAAIDHMRDWINGSGGDWVSMGLCSDGSYGIPEGLFCGSPAICAEGQYARVADLKIRGYARVMLDRTVAELIEERE
AVRALVS
>Mature_326_residues
TGHVKQIAVTGAAGQICYSLLFRIARGELYGPRQPISLRLLDLPQVQPALRGVVMELEDGAFPLLADVAVTDDARLAFRD
IDAAILVGSRPRSKGMERRDLLATNAEIFRLQGRALNDVAKLDAKVLVVGNPANTNAMILSEHAPEFPSENITSMIRLDH
NRALAQLARKALVGLEEIKGLVVWGNHSPTMFVDWSHATVAGRPLADIVADHDWYHKTLIPQVARRGTAVIEARGASSAA
SAANAAIDHMRDWINGSGGDWVSMGLCSDGSYGIPEGLFCGSPAICAEGQYARVADLKIRGYARVMLDRTVAELIEEREA
VRALVS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=327, Percent_Identity=47.0948012232416, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI89886456, Length=327, Percent_Identity=26.2996941896024, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17561064, Length=320, Percent_Identity=47.5, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI32566798, Length=257, Percent_Identity=49.4163424124514, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=43.5294117647059, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24583394, Length=319, Percent_Identity=48.58934169279, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35335; Mature: 35204

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHVKQIAVTGAAGQICYSLLFRIARGELYGPRQPISLRLLDLPQVQPALRGVVMELED
CCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHEEEEECC
GAFPLLADVAVTDDARLAFRDIDAAILVGSRPRSKGMERRDLLATNAEIFRLQGRALNDV
CCCCEEEEEEECCCCEEEEEECCEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCHHHHH
AKLDAKVLVVGNPANTNAMILSEHAPEFPSENITSMIRLDHNRALAQLARKALVGLEEIK
HHCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCHHHCC
GLVVWGNHSPTMFVDWSHATVAGRPLADIVADHDWYHKTLIPQVARRGTAVIEARGASSA
EEEEECCCCCEEEEECCCCEECCCCHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCHH
ASAANAAIDHMRDWINGSGGDWVSMGLCSDGSYGIPEGLFCGSPAICAEGQYARVADLKI
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCCEEEEEEEEE
RGYARVMLDRTVAELIEEREAVRALVS
CHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGHVKQIAVTGAAGQICYSLLFRIARGELYGPRQPISLRLLDLPQVQPALRGVVMELED
CCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHEEEEECC
GAFPLLADVAVTDDARLAFRDIDAAILVGSRPRSKGMERRDLLATNAEIFRLQGRALNDV
CCCCEEEEEEECCCCEEEEEECCEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCHHHHH
AKLDAKVLVVGNPANTNAMILSEHAPEFPSENITSMIRLDHNRALAQLARKALVGLEEIK
HHCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCHHHCC
GLVVWGNHSPTMFVDWSHATVAGRPLADIVADHDWYHKTLIPQVARRGTAVIEARGASSA
EEEEECCCCCEEEEECCCCEECCCCHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCHH
ASAANAAIDHMRDWINGSGGDWVSMGLCSDGSYGIPEGLFCGSPAICAEGQYARVADLKI
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEECCCCEEEEEEEEE
RGYARVMLDRTVAELIEEREAVRALVS
CHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA