Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 154244160

GI number: 154244160

Start: 203078

End: 203779

Strand: Direct

Name: phoB [H]

Synonym: Xaut_0202

Alternate gene names: 154244160

Gene position: 203078-203779 (Clockwise)

Preceding gene: 154244159

Following gene: 154244164

Centisome position: 3.83

GC content: 68.95

Gene sequence:

>702_bases
GTGCCGGCGCATATTCTGATCGTCGAGGATGAGGAGCCCCTGACCCTCCTGCTCCGCTATAATCTGGAGTCGGAAGGCTA
CGGCGTGGAAAGCGTCGGCCGCGGCGACGAGGCCGAGACGCGGCTGCGTGAGAGCGTGCCTGATCTCGTCATCCTCGACT
GGATGCTGCCGGGCCTGTCCGGCATCGAGCTGTGCCGGCGTCTGCGCACCCGTCCCGAGACCGAGCGCTTGCCCGTGATC
ATGCTCACCGCCCGCGGCGAGGAGACCGAGCGTGTGCGCGGCCTTGCCACCGGCGCCGACGATTATGTGGTGAAGCCGTT
CTCGGTGCCCGAATTGCTCGCCCGCGTTCGCGCCCTGCTGCGCCGGGCCAAGCCGGAGCACGTGGCCACCCTGCTGCGCG
CCGGCGACATCGAACTCGACCGGGAGACCCACCGGGTCCACCGCGCCGGCCGCGAGGTGCATCTCGGCCCCACCGAGTTC
CGCCTGCTCGAATTCCTGATGCAGAGCCCCGGCCGCGTGTTCTCCCGCGAGCAGTTGCTCGACGGGGTGTGGGGCCAGGA
CGTCTATATCGACGAGCGCACGGTGGACGTGCATGTGGGCCGCCTGCGCAAGGCCATCAACCGCGGCCGCCAGTCCGATC
CCATCCGCACCGTCCGTGGCGCCGGCTATTCCTTCAACGAGATGTTCGCCCGCTCCCATTGA

Upstream 100 bases:

>100_bases
GTTCCGCCCAGGCCTGATGGGCTGAGCGGACGTGATGGCTGCTCCCGATCCGGATCCACCCGGGTCGGGCGGCCGAAGTA
GTGAAGACAGGTGATAGACA

Downstream 100 bases:

>100_bases
GGGGCGGCGCCTTAAGACGAGGGAGCGCCGTCACCCTCCGGCTTGACCGGAGGGTCCATGGAACGGCCGGGATGCTGCGG
CCGGGGTTTGGACAAGCGGC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPAHILIVEDEEPLTLLLRYNLESEGYGVESVGRGDEAETRLRESVPDLVILDWMLPGLSGIELCRRLRTRPETERLPVI
MLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALLRRAKPEHVATLLRAGDIELDRETHRVHRAGREVHLGPTEF
RLLEFLMQSPGRVFSREQLLDGVWGQDVYIDERTVDVHVGRLRKAINRGRQSDPIRTVRGAGYSFNEMFARSH

Sequences:

>Translated_233_residues
MPAHILIVEDEEPLTLLLRYNLESEGYGVESVGRGDEAETRLRESVPDLVILDWMLPGLSGIELCRRLRTRPETERLPVI
MLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALLRRAKPEHVATLLRAGDIELDRETHRVHRAGREVHLGPTEF
RLLEFLMQSPGRVFSREQLLDGVWGQDVYIDERTVDVHVGRLRKAINRGRQSDPIRTVRGAGYSFNEMFARSH
>Mature_232_residues
PAHILIVEDEEPLTLLLRYNLESEGYGVESVGRGDEAETRLRESVPDLVILDWMLPGLSGIELCRRLRTRPETERLPVIM
LTARGEETERVRGLATGADDYVVKPFSVPELLARVRALLRRAKPEHVATLLRAGDIELDRETHRVHRAGREVHLGPTEFR
LLEFLMQSPGRVFSREQLLDGVWGQDVYIDERTVDVHVGRLRKAINRGRQSDPIRTVRGAGYSFNEMFARSH

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=48.9082969432314, Blast_Score=223, Evalue=8e-60,
Organism=Escherichia coli, GI1790860, Length=227, Percent_Identity=45.8149779735683, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=39.8230088495575, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786784, Length=219, Percent_Identity=38.3561643835616, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786911, Length=236, Percent_Identity=40.2542372881356, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=36.9098712446352, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=38.0090497737557, Blast_Score=136, Evalue=9e-34,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=38.1165919282511, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=39.7260273972603, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=33.0316742081448, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=37.8854625550661, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=35.0649350649351, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=34.7826086956522, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26594; Mature: 26463

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHILIVEDEEPLTLLLRYNLESEGYGVESVGRGDEAETRLRESVPDLVILDWMLPGLS
CCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHCCCC
GIELCRRLRTRPETERLPVIMLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALL
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRAKPEHVATLLRAGDIELDRETHRVHRAGREVHLGPTEFRLLEFLMQSPGRVFSREQLL
HHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHH
DGVWGQDVYIDERTVDVHVGRLRKAINRGRQSDPIRTVRGAGYSFNEMFARSH
CCCCCCCEEEECCEEEHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
PAHILIVEDEEPLTLLLRYNLESEGYGVESVGRGDEAETRLRESVPDLVILDWMLPGLS
CCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHCCCC
GIELCRRLRTRPETERLPVIMLTARGEETERVRGLATGADDYVVKPFSVPELLARVRALL
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHH
RRAKPEHVATLLRAGDIELDRETHRVHRAGREVHLGPTEFRLLEFLMQSPGRVFSREQLL
HHCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHH
DGVWGQDVYIDERTVDVHVGRLRKAINRGRQSDPIRTVRGAGYSFNEMFARSH
CCCCCCCEEEECCEEEHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]