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The map label for this gene is cspA [H]

Identifier: 154244150

GI number: 154244150

Start: 193225

End: 193950

Strand: Direct

Name: cspA [H]

Synonym: Xaut_0192

Alternate gene names: 154244150

Gene position: 193225-193950 (Clockwise)

Preceding gene: 154244148

Following gene: 154244151

Centisome position: 3.64

GC content: 69.56

Gene sequence:

>726_bases
ATGGCCCTAAGCCAAAATTGGCGACTGACCTCAACGGAAATACACATGAGCCGTAACCGAGACTTCCGAGAGCCGCGCCG
CCGTGGCTTTGATGACGACTTTGCGCCGCCGCGTGATCGCGGCTTCGGAGGGGACCGTCCGTTTTCTTCGCCCAGCAGCA
GCTTCAGCGCACCCGCCCCTTCCGGCCCGCCGATCGACGCTACCGTCAAGTGGTTCAACCCCGAGAAGGGCTTCGGCTTT
GTCGAGCTCGCCGATGGCTCGGGTGACGTGTTCCTCCACGCGCGCGCCCTCGAGGCGGCCGGGCAGGAAAGCGTGCCTCC
CGGCTCCAAGCTCTCGGTCCGCGTCGGCCAGGGCCAGAAGGGCCGCCAGGTGACCGAGGTCCTCGAGGTTGACACCTCCA
CGGCCGAGGCCGCGCCTGCGCGTCGCAGCTTCGGCGCCGGTCCCGGTGCTGGCGGCGGCTTCGGCGGCGCCCCCCGCGCC
GGCGGCGCTGGCCCCCGTGCGGCCACCGGCCCCACCGAGGAGCGCGTCGGTACCGTGAAGTGGTACAACCCCGACAAGGG
TTTTGGCTTCATCGCGGTGGAAGGCGGCGGCAAGGACGTGTTCGTGCACGTCACCGTCATCTCGCGTTCCGGCCTCACCG
ACCTGGCCGAAGGCCAGCGCGTGGTGGTGCAGGTGGGTCAGGGTCCCAAGGGTCCTGAAGCCCGCGGCATCGAGCTCGCC
GACTGA

Upstream 100 bases:

>100_bases
ACGCGGGTGGCTGGATTGAGCGTTGTGCGAAGGCGGATTTCAAGACGCCGGGAACGTGCAAGTTAAAGCATGAGAAACGC
ATTTCATCCGGCAGTGGTGG

Downstream 100 bases:

>100_bases
TCATTCATCCTCCCGCCGTCCTTCGGGACGGCGGGATTGGATCCACTGAACGGGCGAGGCTTCCCGCTTCGCCTGTTTTT
GGCATTTCTGGAGTTCAAGA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MALSQNWRLTSTEIHMSRNRDFREPRRRGFDDDFAPPRDRGFGGDRPFSSPSSSFSAPAPSGPPIDATVKWFNPEKGFGF
VELADGSGDVFLHARALEAAGQESVPPGSKLSVRVGQGQKGRQVTEVLEVDTSTAEAAPARRSFGAGPGAGGGFGGAPRA
GGAGPRAATGPTEERVGTVKWYNPDKGFGFIAVEGGGKDVFVHVTVISRSGLTDLAEGQRVVVQVGQGPKGPEARGIELA
D

Sequences:

>Translated_241_residues
MALSQNWRLTSTEIHMSRNRDFREPRRRGFDDDFAPPRDRGFGGDRPFSSPSSSFSAPAPSGPPIDATVKWFNPEKGFGF
VELADGSGDVFLHARALEAAGQESVPPGSKLSVRVGQGQKGRQVTEVLEVDTSTAEAAPARRSFGAGPGAGGGFGGAPRA
GGAGPRAATGPTEERVGTVKWYNPDKGFGFIAVEGGGKDVFVHVTVISRSGLTDLAEGQRVVVQVGQGPKGPEARGIELA
D
>Mature_240_residues
ALSQNWRLTSTEIHMSRNRDFREPRRRGFDDDFAPPRDRGFGGDRPFSSPSSSFSAPAPSGPPIDATVKWFNPEKGFGFV
ELADGSGDVFLHARALEAAGQESVPPGSKLSVRVGQGQKGRQVTEVLEVDTSTAEAAPARRSFGAGPGAGGGFGGAPRAG
GAGPRAATGPTEERVGTVKWYNPDKGFGFIAVEGGGKDVFVHVTVISRSGLTDLAEGQRVVVQVGQGPKGPEARGIELAD

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=54.0983606557377, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787839, Length=70, Percent_Identity=44.2857142857143, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=50.8196721311475, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787107, Length=61, Percent_Identity=54.0983606557377, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367114, Length=66, Percent_Identity=45.4545454545455, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787834, Length=66, Percent_Identity=45.4545454545455, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=50.8196721311475, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 25203; Mature: 25072

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSQNWRLTSTEIHMSRNRDFREPRRRGFDDDFAPPRDRGFGGDRPFSSPSSSFSAPAP
CCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGPPIDATVKWFNPEKGFGFVELADGSGDVFLHARALEAAGQESVPPGSKLSVRVGQGQK
CCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEEHHHCCCCCCCCCCEEEEEECCCCC
GRQVTEVLEVDTSTAEAAPARRSFGAGPGAGGGFGGAPRAGGAGPRAATGPTEERVGTVK
CCHHHHHHHCCCCCHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEE
WYNPDKGFGFIAVEGGGKDVFVHVTVISRSGLTDLAEGQRVVVQVGQGPKGPEARGIELA
EECCCCCEEEEEEECCCCEEEEEEEEEECCCCCHHHCCCEEEEEECCCCCCCCCCCCEEC
D
C
>Mature Secondary Structure 
ALSQNWRLTSTEIHMSRNRDFREPRRRGFDDDFAPPRDRGFGGDRPFSSPSSSFSAPAP
CCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SGPPIDATVKWFNPEKGFGFVELADGSGDVFLHARALEAAGQESVPPGSKLSVRVGQGQK
CCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEEEHHHCCCCCCCCCCEEEEEECCCCC
GRQVTEVLEVDTSTAEAAPARRSFGAGPGAGGGFGGAPRAGGAGPRAATGPTEERVGTVK
CCHHHHHHHCCCCCHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEE
WYNPDKGFGFIAVEGGGKDVFVHVTVISRSGLTDLAEGQRVVVQVGQGPKGPEARGIELA
EECCCCCEEEEEEECCCCEEEEEEEEEECCCCCHHHCCCEEEEEECCCCCCCCCCCCEEC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10483731 [H]