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The map label for this gene is hyfB [H]

Identifier: 154244124

GI number: 154244124

Start: 161793

End: 163838

Strand: Direct

Name: hyfB [H]

Synonym: Xaut_0166

Alternate gene names: 154244124

Gene position: 161793-163838 (Clockwise)

Preceding gene: 154244123

Following gene: 154244125

Centisome position: 3.05

GC content: 70.63

Gene sequence:

>2046_bases
ATGCCCGATGCCGCCTTCGCCGCACAGCTTCCCGTCAGCGGACAACTCCTCGTGGCCCTCGGCGCGGTGGCGGTGCTTCT
GGCTCTCGCCGGGCTCGGTGTGGCGACGGCGCGGCGCCCCGCGGGCCGGCCGCTCGTCTATGGCGGCGCGCTGGCGGTGA
CACTTTTGGCCGGTCTCTCCGCCCTTACCGCCATCGCGGCGCCGGCGGCTGGGATCGTGCTGCCCCTTGGCATTCCCTGG
ATCGGCGCCCACCTGCGGCTCGATGCGTTGTCTGCGGTCTTCCTCGCGGTGATCAATCTCGGCGGCGCGGCGGCGAGCCT
CTATGGCCTCGGCTACGGCCGGCACGATCATGCGCCGGAACGGGTGCTGCCCTTCTTCCCGGCCTTCCTCGCCGGCATGA
ACATGGTGCTGCTGGCGGACGACGCCTACAGTTTCCTCCTGTCGTGGGAATTCATGTCGCTGGCGTCCTGGGCGCTGGTG
GTGGCCCACGACAAGGAGGCGGGCAATGTGCGCGCCGGCCTCGTCTACATCCTCATGGCCTCGTTCGGCACGCTGGCGCT
GATGCTGGCCTTTGGCCTGCTCGCCGCCGGCTCCGGGGGCGGCTATGCCTTCGATGCCATGCGCGAGGCGCCGCCCGTGG
GCGCCTTCGCCGCGCTGGTGCTGGCGCTTGCGCTGCTGGGGGCGGGGTCCAAGGCGGGTCTGATGCCGCTGCACGTCTGG
CTGCCGCTGGCCCATCCCGCCGCGCCCAGCCACGTCTCCGCCCTCATGAGCGGGGTGATGACCAAGGTGGCGGTCTACGG
CTTCATCCGCATCGTGTTTGATCTCTCCGGCCCGCCGGACTGGTGGTGGGGCATCCCGGTGCTGGGTGCCGGCGGCGTCG
CGGCGGTCATCGGCGTGCTGCAGGCGCTGTTGCAGGGCGACACCAAGCGCGTGCTCGCCTATTCCACCATCGAGAATATC
GGCCTCATTTTCATCGGCCTCGGGCTGGCGCTGGCCTTCTCGGCCAACGGATTTCCCACCGCCGCCGCGCTGGCGCTCAC
GGCGGCGCTGTTCCATGTGCTCAACCATGCGGCCATGAAGAGCCTGCTGTTCTTCGGTGCCGGCGCTGTGCTGACCGCCA
CCGGCACCCGGCAGATGGAGGAACTGGGCGGCCTCATCCACCGCATGCCCACCACCAGCGTGGTGATGCTGATCGGCTGC
GCCGCCATCTCGGCCCTGCCGCCGCTCAATGGTTTTGCCTCCGAATGGCTGGTGTTTCAGGCGGTGCTGCTGAGCCCGGA
GCTTCCGGTCTGGGGGCTGAAGCTGATGGTGCCGGCCGTGGGCGCGCTGATGGCGCTCGCCGCCGCGCTCGCCGCCGCCT
GCTTCGTGCGGCTCTACGGCATCGCCTTCCTCGGCCGGCCGCGCAGCCCGAAGGCGAGCGAGGCCCACGAGGTGGACGCC
TTCTCCCGTGCCGCCATGATCGCGCTTGCCGCCGTGTGCGTGGGCGCCGGCCTGCTGCCCGGCTTTGTCATCGATGTGCT
GCAGCCGGCGGTGGGCGGCCTCGTGGGCCAGACCATGCCGGCGCAGGGCTCAGTGGCGTGGCTCTCCATCATCCCGGTGG
CGGAGAGCCGCAGCTCCTACAACGGGCTGCTGGTCTTTGCCTTCACGGCACTGTCGGCGCTGCTGGCGGCCTTCGTCATC
CACCGCTTCGCCTCGGGCGCGGTACGGCGGGCGCCGCCGTGGGATTGCGGCTTCCCCAATGCCAGCCCGCTGAGCCAGTA
TTCCGCCGCCTCCTTCGCCCAGCCCATCCGCCGGGTGTTCGCGCGCGGCCTGTTCGCGGCCCGCGAGAAGGTGGAGATGC
CGCCCCCCGGCGCGCTCACTCCCGCCCGGTTCTCCTCCAGCGCGCGCGATCCCGCCTGGGCCTTCCTGTTCCTGCCCCTG
ACGGCGGCGGTGGACTATGTGGCCGAGCGGGCGAACCGGCTGCAATACCTCACCATCCGCCAGTATCTGGGCCTGGTTTT
TTCGGCCCTGGTGCTGCTGCTTCTGGTGCTCGCCCTATGGACCTGA

Upstream 100 bases:

>100_bases
GGCCGTCAGCGACGCTGGCGGGCGGGGCGTGCGCCTGCGGGTGCAAATGCCTGCCCAATCGATCCTTGCCGCCCGGCGGC
CGGCGGAGGAGGAGTAGGCC

Downstream 100 bases:

>100_bases
TGGCGCACATCCTGGTGCAGGGCACGCAGATGCTGCTCGTGCTCGCCCTCGCCCCGCTCCTCACCGGGCTGGTGCGCAAG
GTGAAGGCGCGCCTCCAGCG

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MPDAAFAAQLPVSGQLLVALGAVAVLLALAGLGVATARRPAGRPLVYGGALAVTLLAGLSALTAIAAPAAGIVLPLGIPW
IGAHLRLDALSAVFLAVINLGGAAASLYGLGYGRHDHAPERVLPFFPAFLAGMNMVLLADDAYSFLLSWEFMSLASWALV
VAHDKEAGNVRAGLVYILMASFGTLALMLAFGLLAAGSGGGYAFDAMREAPPVGAFAALVLALALLGAGSKAGLMPLHVW
LPLAHPAAPSHVSALMSGVMTKVAVYGFIRIVFDLSGPPDWWWGIPVLGAGGVAAVIGVLQALLQGDTKRVLAYSTIENI
GLIFIGLGLALAFSANGFPTAAALALTAALFHVLNHAAMKSLLFFGAGAVLTATGTRQMEELGGLIHRMPTTSVVMLIGC
AAISALPPLNGFASEWLVFQAVLLSPELPVWGLKLMVPAVGALMALAAALAAACFVRLYGIAFLGRPRSPKASEAHEVDA
FSRAAMIALAAVCVGAGLLPGFVIDVLQPAVGGLVGQTMPAQGSVAWLSIIPVAESRSSYNGLLVFAFTALSALLAAFVI
HRFASGAVRRAPPWDCGFPNASPLSQYSAASFAQPIRRVFARGLFAAREKVEMPPPGALTPARFSSSARDPAWAFLFLPL
TAAVDYVAERANRLQYLTIRQYLGLVFSALVLLLLVLALWT

Sequences:

>Translated_681_residues
MPDAAFAAQLPVSGQLLVALGAVAVLLALAGLGVATARRPAGRPLVYGGALAVTLLAGLSALTAIAAPAAGIVLPLGIPW
IGAHLRLDALSAVFLAVINLGGAAASLYGLGYGRHDHAPERVLPFFPAFLAGMNMVLLADDAYSFLLSWEFMSLASWALV
VAHDKEAGNVRAGLVYILMASFGTLALMLAFGLLAAGSGGGYAFDAMREAPPVGAFAALVLALALLGAGSKAGLMPLHVW
LPLAHPAAPSHVSALMSGVMTKVAVYGFIRIVFDLSGPPDWWWGIPVLGAGGVAAVIGVLQALLQGDTKRVLAYSTIENI
GLIFIGLGLALAFSANGFPTAAALALTAALFHVLNHAAMKSLLFFGAGAVLTATGTRQMEELGGLIHRMPTTSVVMLIGC
AAISALPPLNGFASEWLVFQAVLLSPELPVWGLKLMVPAVGALMALAAALAAACFVRLYGIAFLGRPRSPKASEAHEVDA
FSRAAMIALAAVCVGAGLLPGFVIDVLQPAVGGLVGQTMPAQGSVAWLSIIPVAESRSSYNGLLVFAFTALSALLAAFVI
HRFASGAVRRAPPWDCGFPNASPLSQYSAASFAQPIRRVFARGLFAAREKVEMPPPGALTPARFSSSARDPAWAFLFLPL
TAAVDYVAERANRLQYLTIRQYLGLVFSALVLLLLVLALWT
>Mature_680_residues
PDAAFAAQLPVSGQLLVALGAVAVLLALAGLGVATARRPAGRPLVYGGALAVTLLAGLSALTAIAAPAAGIVLPLGIPWI
GAHLRLDALSAVFLAVINLGGAAASLYGLGYGRHDHAPERVLPFFPAFLAGMNMVLLADDAYSFLLSWEFMSLASWALVV
AHDKEAGNVRAGLVYILMASFGTLALMLAFGLLAAGSGGGYAFDAMREAPPVGAFAALVLALALLGAGSKAGLMPLHVWL
PLAHPAAPSHVSALMSGVMTKVAVYGFIRIVFDLSGPPDWWWGIPVLGAGGVAAVIGVLQALLQGDTKRVLAYSTIENIG
LIFIGLGLALAFSANGFPTAAALALTAALFHVLNHAAMKSLLFFGAGAVLTATGTRQMEELGGLIHRMPTTSVVMLIGCA
AISALPPLNGFASEWLVFQAVLLSPELPVWGLKLMVPAVGALMALAAALAAACFVRLYGIAFLGRPRSPKASEAHEVDAF
SRAAMIALAAVCVGAGLLPGFVIDVLQPAVGGLVGQTMPAQGSVAWLSIIPVAESRSSYNGLLVFAFTALSALLAAFVIH
RFASGAVRRAPPWDCGFPNASPLSQYSAASFAQPIRRVFARGLFAAREKVEMPPPGALTPARFSSSARDPAWAFLFLPLT
AAVDYVAERANRLQYLTIRQYLGLVFSALVLLLLVLALWT

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=429, Percent_Identity=24.2424242424242, Blast_Score=94, Evalue=6e-19,
Organism=Escherichia coli, GI1788827, Length=609, Percent_Identity=37.1100164203612, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI2367154, Length=524, Percent_Identity=30.5343511450382, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI1788831, Length=538, Percent_Identity=29.7397769516729, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788829, Length=403, Percent_Identity=32.258064516129, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788614, Length=343, Percent_Identity=32.0699708454811, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788613, Length=463, Percent_Identity=23.110151187905, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70416; Mature: 70285

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDAAFAAQLPVSGQLLVALGAVAVLLALAGLGVATARRPAGRPLVYGGALAVTLLAGLS
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEECHHHHHHHHHHHH
ALTAIAAPAAGIVLPLGIPWIGAHLRLDALSAVFLAVINLGGAAASLYGLGYGRHDHAPE
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCH
RVLPFFPAFLAGMNMVLLADDAYSFLLSWEFMSLASWALVVAHDKEAGNVRAGLVYILMA
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHH
SFGTLALMLAFGLLAAGSGGGYAFDAMREAPPVGAFAALVLALALLGAGSKAGLMPLHVW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
LPLAHPAAPSHVSALMSGVMTKVAVYGFIRIVFDLSGPPDWWWGIPVLGAGGVAAVIGVL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHH
QALLQGDTKRVLAYSTIENIGLIFIGLGLALAFSANGFPTAAALALTAALFHVLNHAAMK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLLFFGAGAVLTATGTRQMEELGGLIHRMPTTSVVMLIGCAAISALPPLNGFASEWLVFQ
HHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AVLLSPELPVWGLKLMVPAVGALMALAAALAAACFVRLYGIAFLGRPRSPKASEAHEVDA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FSRAAMIALAAVCVGAGLLPGFVIDVLQPAVGGLVGQTMPAQGSVAWLSIIPVAESRSSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCC
NGLLVFAFTALSALLAAFVIHRFASGAVRRAPPWDCGFPNASPLSQYSAASFAQPIRRVF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARGLFAAREKVEMPPPGALTPARFSSSARDPAWAFLFLPLTAAVDYVAERANRLQYLTIR
HHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLGLVFSALVLLLLVLALWT
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDAAFAAQLPVSGQLLVALGAVAVLLALAGLGVATARRPAGRPLVYGGALAVTLLAGLS
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCEEECHHHHHHHHHHHH
ALTAIAAPAAGIVLPLGIPWIGAHLRLDALSAVFLAVINLGGAAASLYGLGYGRHDHAPE
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCH
RVLPFFPAFLAGMNMVLLADDAYSFLLSWEFMSLASWALVVAHDKEAGNVRAGLVYILMA
HHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHH
SFGTLALMLAFGLLAAGSGGGYAFDAMREAPPVGAFAALVLALALLGAGSKAGLMPLHVW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
LPLAHPAAPSHVSALMSGVMTKVAVYGFIRIVFDLSGPPDWWWGIPVLGAGGVAAVIGVL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHH
QALLQGDTKRVLAYSTIENIGLIFIGLGLALAFSANGFPTAAALALTAALFHVLNHAAMK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLLFFGAGAVLTATGTRQMEELGGLIHRMPTTSVVMLIGCAAISALPPLNGFASEWLVFQ
HHHHHCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AVLLSPELPVWGLKLMVPAVGALMALAAALAAACFVRLYGIAFLGRPRSPKASEAHEVDA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FSRAAMIALAAVCVGAGLLPGFVIDVLQPAVGGLVGQTMPAQGSVAWLSIIPVAESRSSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCC
NGLLVFAFTALSALLAAFVIHRFASGAVRRAPPWDCGFPNASPLSQYSAASFAQPIRRVF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARGLFAAREKVEMPPPGALTPARFSSSARDPAWAFLFLPLTAAVDYVAERANRLQYLTIR
HHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYLGLVFSALVLLLLVLALWT
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]