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The map label for this gene is mdh

Identifier: 154244111

GI number: 154244111

Start: 147393

End: 148358

Strand: Direct

Name: mdh

Synonym: Xaut_0153

Alternate gene names: 154244111

Gene position: 147393-148358 (Clockwise)

Preceding gene: 154244110

Following gene: 154244112

Centisome position: 2.78

GC content: 67.81

Gene sequence:

>966_bases
ATGGCCCGCAACAAGATCGCCCTCATCGGGGCCGGCCAGATCGGCGGTACGCTCGCCCTTCTCGCCGGGATGAAGGCGTT
GGGCGACATCGTCCTGTTCGACGTCGCCGAGGGCGTGCCCGAGGGCAAGGCCCTCGACCTTGCGGAACTGACCCCCGTCG
AAGGCTTTGATGCCGCCTATGCCGGCGCGTCGAGCTATGATGCCATCTCCGGTGCCGACGTGGTGATCGTCACCGCCGGG
GTGGCGCGCAAGCCGGGCATGAGCCGCGACGACCTCCTCGCCATCAACCTCAAGGTGATGGAGCAGGTGGGCGCCGGCAT
CCGCAAATATGCCCCTGACGCTTTCGTCATCTGCATCACCAATCCGCTCGATGCCATGGTGTGGGCGCTCCAGCGCTCCA
GCGGCCTGCCGCCGGAAAAGGTGGTGGGCATGGCCGGCGTGCTGGATTCCGCGCGCCTGCGCTATTTCCTCGCCGAGGAG
TTCGGCGTCTCGGTGGAGGACGTGACCGCCTTCGTCATGGGCGGGCACGGCGACACCATGGTGCCGCTGGTACGCTACTC
CACCGTGGGCGGCATCCCGGTGCCGGATCTCATCCGCATGGGCTGGACCAGCGAGGAGCGCATCGCCGCCATCGTCCAGC
GCACCCGCGACGGCGGGGCGGAGATCGTCAACCTGCTCAAGAGCGGCTCGGCCTTTTATGCGCCGGCGGCGTCCGCCATC
GCCATGGCAGAAAGCTACCTGCGGGACAAGAAGCGCGTCCTCCCCGTCGCAGCACTGCTCGACGGGGAATACGGGCTCAG
GGACATCTATGTGGGCGTGCCGGCGGTGATCGGCGCGCGTGGCGTGGAGCGCATCGTGGAGGTCGAGCTCGACCGCTCCG
AGCGCGCCATGTTCGACAGATCGGTCGCTGCCGTCGAAGGACTGGTGGAGGCCTGCCTGAAGATCGCACCCGGACTCGGG
AAGTGA

Upstream 100 bases:

>100_bases
GCTTGAACCCCGGGGCGGATGGGAGTAGTCAAGCGCCGCTCGTTTCCTGCGGCGGCTCGTCTCATGCGCCCGCCGGCAGA
TCGGTTTCCGGGGACAAGGG

Downstream 100 bases:

>100_bases
TCTCGACAGTGACGGCGCGCCGAAGGGTGCGCCGTTTTCGTGCCGGCGGAACGACCGGTATGGTGAAGGAGACGAATGCT
CCCCTTCCGGGGACCCCCAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MARNKIALIGAGQIGGTLALLAGMKALGDIVLFDVAEGVPEGKALDLAELTPVEGFDAAYAGASSYDAISGADVVIVTAG
VARKPGMSRDDLLAINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQRSSGLPPEKVVGMAGVLDSARLRYFLAEE
FGVSVEDVTAFVMGGHGDTMVPLVRYSTVGGIPVPDLIRMGWTSEERIAAIVQRTRDGGAEIVNLLKSGSAFYAPAASAI
AMAESYLRDKKRVLPVAALLDGEYGLRDIYVGVPAVIGARGVERIVEVELDRSERAMFDRSVAAVEGLVEACLKIAPGLG
K

Sequences:

>Translated_321_residues
MARNKIALIGAGQIGGTLALLAGMKALGDIVLFDVAEGVPEGKALDLAELTPVEGFDAAYAGASSYDAISGADVVIVTAG
VARKPGMSRDDLLAINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQRSSGLPPEKVVGMAGVLDSARLRYFLAEE
FGVSVEDVTAFVMGGHGDTMVPLVRYSTVGGIPVPDLIRMGWTSEERIAAIVQRTRDGGAEIVNLLKSGSAFYAPAASAI
AMAESYLRDKKRVLPVAALLDGEYGLRDIYVGVPAVIGARGVERIVEVELDRSERAMFDRSVAAVEGLVEACLKIAPGLG
K
>Mature_320_residues
ARNKIALIGAGQIGGTLALLAGMKALGDIVLFDVAEGVPEGKALDLAELTPVEGFDAAYAGASSYDAISGADVVIVTAGV
ARKPGMSRDDLLAINLKVMEQVGAGIRKYAPDAFVICITNPLDAMVWALQRSSGLPPEKVVGMAGVLDSARLRYFLAEEF
GVSVEDVTAFVMGGHGDTMVPLVRYSTVGGIPVPDLIRMGWTSEERIAAIVQRTRDGGAEIVNLLKSGSAFYAPAASAIA
MAESYLRDKKRVLPVAALLDGEYGLRDIYVGVPAVIGARGVERIVEVELDRSERAMFDRSVAAVEGLVEACLKIAPGLGK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=30.4918032786885, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=30.4918032786885, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI5031857, Length=319, Percent_Identity=29.7805642633229, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI9257228, Length=304, Percent_Identity=30.2631578947368, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4504973, Length=304, Percent_Identity=30.2631578947368, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI260099723, Length=319, Percent_Identity=29.7805642633229, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI291575128, Length=308, Percent_Identity=29.5454545454545, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI4557032, Length=308, Percent_Identity=29.5454545454545, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=24.9180327868852, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI260099727, Length=200, Percent_Identity=31.5, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI260099725, Length=207, Percent_Identity=32.3671497584541, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI21735621, Length=293, Percent_Identity=28.3276450511945, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=28.7179487179487, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI103472011, Length=289, Percent_Identity=21.4532871972318, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789632, Length=293, Percent_Identity=31.3993174061433, Blast_Score=98, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=282, Percent_Identity=25.531914893617, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=313, Percent_Identity=32.2683706070287, Blast_Score=110, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6322765, Length=311, Percent_Identity=27.9742765273312, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324446, Length=254, Percent_Identity=29.9212598425197, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320125, Length=241, Percent_Identity=27.8008298755187, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136226, Length=317, Percent_Identity=27.1293375394322, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550422, Length=314, Percent_Identity=25.1592356687898, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24647881, Length=333, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663599, Length=314, Percent_Identity=27.0700636942675, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663595, Length=236, Percent_Identity=31.7796610169492, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_XANP2 (A7IBL9)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001415069.1
- ProteinModelPortal:   A7IBL9
- SMR:   A7IBL9
- STRING:   A7IBL9
- GeneID:   5422091
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_0153
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   LDKPAND
- ProtClustDB:   PRK06223
- BioCyc:   XAUT78245:XAUT_0153-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33664; Mature: 33533

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKIALIGAGQIGGTLALLAGMKALGDIVLFDVAEGVPEGKALDLAELTPVEGFDAAY
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCHHHCCCCCCCCHHH
AGASSYDAISGADVVIVTAGVARKPGMSRDDLLAINLKVMEQVGAGIRKYAPDAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHCCCEEEEEEC
NPLDAMVWALQRSSGLPPEKVVGMAGVLDSARLRYFLAEEFGVSVEDVTAFVMGGHGDTM
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCE
VPLVRYSTVGGIPVPDLIRMGWTSEERIAAIVQRTRDGGAEIVNLLKSGSAFYAPAASAI
EHHEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCHHHHH
AMAESYLRDKKRVLPVAALLDGEYGLRDIYVGVPAVIGARGVERIVEVELDRSERAMFDR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHH
SVAAVEGLVEACLKIAPGLGK
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARNKIALIGAGQIGGTLALLAGMKALGDIVLFDVAEGVPEGKALDLAELTPVEGFDAAY
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEHHHCCCCCCCCCCHHHCCCCCCCCHHH
AGASSYDAISGADVVIVTAGVARKPGMSRDDLLAINLKVMEQVGAGIRKYAPDAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHCCCEEEEEEC
NPLDAMVWALQRSSGLPPEKVVGMAGVLDSARLRYFLAEEFGVSVEDVTAFVMGGHGDTM
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCE
VPLVRYSTVGGIPVPDLIRMGWTSEERIAAIVQRTRDGGAEIVNLLKSGSAFYAPAASAI
EHHEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCHHHHH
AMAESYLRDKKRVLPVAALLDGEYGLRDIYVGVPAVIGARGVERIVEVELDRSERAMFDR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHH
SVAAVEGLVEACLKIAPGLGK
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA