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The map label for this gene is nifB [H]

Identifier: 154244069

GI number: 154244069

Start: 115938

End: 117500

Strand: Direct

Name: nifB [H]

Synonym: Xaut_0111

Alternate gene names: 154244069

Gene position: 115938-117500 (Clockwise)

Preceding gene: 154244068

Following gene: 154244070

Centisome position: 2.18

GC content: 64.75

Gene sequence:

>1563_bases
ATGCACGCGTCGACACAGCACGAGACGACCGAAGTCGGTGTGGCCGACAAGCTGGCCGAAGTCATGCAGGCGAAGGCCGA
ACACCAGGGTTGCGGCACGTCCGGCGGCTCGGGCAAGGCCAGCTGCGGTTCGTCCGCCGGTCAGGGCGACCTGCCCACCG
AGATCTGGGAGAAGGTGAAGAACCATCCCTGCTATTCTGAAGAAGCCCACCACCACTATGCGCGTATGCATGTTGCCGTT
GCCCCGGCGTGCAACATCCAGTGCAACTACTGCAATCGCAAGTATGATTGCGCCAACGAATCCCGCCCCGGCGTGGTGTC
CGAGAAGCTGACGCCCCAGCAGGCGGCGAAGAAGGTGCTCGCGGTCGCCTCCACCATCCCGCAGATGACCGTGCTGGGCA
TCGCCGGCCCCGGCGACCCGCTCGCCAATCCGGAGAAGACCTTCCAGACCTTCGAGCTGATCAACCGGACCGCCCCGGAC
ATCAAGCTCTGCCTGTCCACCAACGGCCTGGCGCTGCCCGACCACGTGGACACCATCGCCGGCTTCAACGTCGACCACGT
GACCATCACCATCAACATGGTGGACCCGGAAGTCGGCGCGAAGATCTATCCGTGGGTGTTCTGGAAGCACAAGCGCTACA
CTGGCTATGAGGCCGCCAAGCTCCTCACCGACCGGCAGATGCTGGGCCTCGAGATGCTCACCGAGCGTGGCATCCTCTGC
AAGGTCAACTCGGTGATGATCCCCGGCATCAACGACAAGCACCTTGTCGAAGTGAACCGGGCGGTGAAGTCCCGCGGCGC
GTTCCTGCATAACATCATGCCGCTCATCTCGGCGCCCGAGCACGGCACCGTGTTCGGCCTCAACGGCCAGCGCGGCCCGA
CGGCCCAGGAGCTCAAGGCGCTCCAGGATTCGTGCGAAGGCGAGATGAACATGATGCGGCATTGCCGCCAGTGCCGCGCC
GACGCCGTGGGCCTCCTCGGCGAGGATCGGTCCGCCGAGTTCACCACCGACAAGATCATGGCCATGGAGGTCAACTACGA
CCTCGACAGCCGCAAGGCATACCAGGCCCTGGTCGAGGAAGAGCGCGTGGCGAAGGTCGCCGCGAAGCAGGAAGAGATGG
AGGCCCTCGCCGGCGAGCAGAGCGACATCAAGCTCCTCGTGGCCGTGGCCACCAAGGGCTCGGGCCTCATCAACGAGCAC
TTCGGCCACGCCAAGGAGTTCCAGGTGTGGGAGCTCTCCACCGCGGGCGCCAAGTTCGTCGGCCATCGCCGCGTCGACCT
GTATTGCCAGGGCGGCTACGGCGAGGAGGACAGCCTCGAGACCATCATCCGCGCCATCAACGACTGCCATGCGGTGTTCG
TGGCCAAGATCGGCGGTTGCCCGAAGGCTGACCTCGTCAAGGCCGGCATCGAGCCGGTGGACGGCTTCGCCCACGAGTTC
ATCGAGAAATCGGCCATCACCTGGTTCAAGGACTACCTCAACAAGGTGAAGACCGGTGAGATCGTCCATGCGGAGCGCGG
CGACGCCGAGATCCGTCAGGGCGCCCTCATCAACGCGGCCTGA

Upstream 100 bases:

>100_bases
GAAAATCATTACGTATTTTTCCGTAGCCCTAGTGGCACGCCAGTTGCTCATCCCTGTCCAAGGATTTTTTTGGATCCTGC
TGGAAGCGAGGAGCAGTTTC

Downstream 100 bases:

>100_bases
TCCTGCCCGCACCAACCAGATCCGCCCAGGCCGCAATGGCCTGGGCGGATCTCCCTGAAGTCCACGCCAACAGACCGTCC
GGCCGACCTGATCGCGCGCT

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MHASTQHETTEVGVADKLAEVMQAKAEHQGCGTSGGSGKASCGSSAGQGDLPTEIWEKVKNHPCYSEEAHHHYARMHVAV
APACNIQCNYCNRKYDCANESRPGVVSEKLTPQQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFQTFELINRTAPD
IKLCLSTNGLALPDHVDTIAGFNVDHVTITINMVDPEVGAKIYPWVFWKHKRYTGYEAAKLLTDRQMLGLEMLTERGILC
KVNSVMIPGINDKHLVEVNRAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKALQDSCEGEMNMMRHCRQCRA
DAVGLLGEDRSAEFTTDKIMAMEVNYDLDSRKAYQALVEEERVAKVAAKQEEMEALAGEQSDIKLLVAVATKGSGLINEH
FGHAKEFQVWELSTAGAKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKADLVKAGIEPVDGFAHEF
IEKSAITWFKDYLNKVKTGEIVHAERGDAEIRQGALINAA

Sequences:

>Translated_520_residues
MHASTQHETTEVGVADKLAEVMQAKAEHQGCGTSGGSGKASCGSSAGQGDLPTEIWEKVKNHPCYSEEAHHHYARMHVAV
APACNIQCNYCNRKYDCANESRPGVVSEKLTPQQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFQTFELINRTAPD
IKLCLSTNGLALPDHVDTIAGFNVDHVTITINMVDPEVGAKIYPWVFWKHKRYTGYEAAKLLTDRQMLGLEMLTERGILC
KVNSVMIPGINDKHLVEVNRAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKALQDSCEGEMNMMRHCRQCRA
DAVGLLGEDRSAEFTTDKIMAMEVNYDLDSRKAYQALVEEERVAKVAAKQEEMEALAGEQSDIKLLVAVATKGSGLINEH
FGHAKEFQVWELSTAGAKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKADLVKAGIEPVDGFAHEF
IEKSAITWFKDYLNKVKTGEIVHAERGDAEIRQGALINAA
>Mature_520_residues
MHASTQHETTEVGVADKLAEVMQAKAEHQGCGTSGGSGKASCGSSAGQGDLPTEIWEKVKNHPCYSEEAHHHYARMHVAV
APACNIQCNYCNRKYDCANESRPGVVSEKLTPQQAAKKVLAVASTIPQMTVLGIAGPGDPLANPEKTFQTFELINRTAPD
IKLCLSTNGLALPDHVDTIAGFNVDHVTITINMVDPEVGAKIYPWVFWKHKRYTGYEAAKLLTDRQMLGLEMLTERGILC
KVNSVMIPGINDKHLVEVNRAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKALQDSCEGEMNMMRHCRQCRA
DAVGLLGEDRSAEFTTDKIMAMEVNYDLDSRKAYQALVEEERVAKVAAKQEEMEALAGEQSDIKLLVAVATKGSGLINEH
FGHAKEFQVWELSTAGAKFVGHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKADLVKAGIEPVDGFAHEF
IEKSAITWFKDYLNKVKTGEIVHAERGDAEIRQGALINAA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56734; Mature: 56734

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHASTQHETTEVGVADKLAEVMQAKAEHQGCGTSGGSGKASCGSSAGQGDLPTEIWEKVK
CCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
NHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPQQAAKKVL
CCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AVASTIPQMTVLGIAGPGDPLANPEKTFQTFELINRTAPDIKLCLSTNGLALPDHVDTIA
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
GFNVDHVTITINMVDPEVGAKIYPWVFWKHKRYTGYEAAKLLTDRQMLGLEMLTERGILC
CCCEEEEEEEEEEECCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
KVNSVMIPGINDKHLVEVNRAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKA
EECCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
LQDSCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDLDSRKAYQALVEE
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
ERVAKVAAKQEEMEALAGEQSDIKLLVAVATKGSGLINEHFGHAKEFQVWELSTAGAKFV
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEECCCCHHHH
GHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKADLVKAGIEPVDGFAHEF
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEHHHHCCCCHHHHHHHCCCCCHHHHHHH
IEKSAITWFKDYLNKVKTGEIVHAERGDAEIRQGALINAA
HHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCEEECCC
>Mature Secondary Structure
MHASTQHETTEVGVADKLAEVMQAKAEHQGCGTSGGSGKASCGSSAGQGDLPTEIWEKVK
CCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
NHPCYSEEAHHHYARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSEKLTPQQAAKKVL
CCCCCCCHHHHHHHHEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AVASTIPQMTVLGIAGPGDPLANPEKTFQTFELINRTAPDIKLCLSTNGLALPDHVDTIA
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHH
GFNVDHVTITINMVDPEVGAKIYPWVFWKHKRYTGYEAAKLLTDRQMLGLEMLTERGILC
CCCEEEEEEEEEEECCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
KVNSVMIPGINDKHLVEVNRAVKSRGAFLHNIMPLISAPEHGTVFGLNGQRGPTAQELKA
EECCEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHH
LQDSCEGEMNMMRHCRQCRADAVGLLGEDRSAEFTTDKIMAMEVNYDLDSRKAYQALVEE
HHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
ERVAKVAAKQEEMEALAGEQSDIKLLVAVATKGSGLINEHFGHAKEFQVWELSTAGAKFV
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHCCCCCCEEEEEECCCCHHHH
GHRRVDLYCQGGYGEEDSLETIIRAINDCHAVFVAKIGGCPKADLVKAGIEPVDGFAHEF
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEHHHHCCCCHHHHHHHCCCCCHHHHHHH
IEKSAITWFKDYLNKVKTGEIVHAERGDAEIRQGALINAA
HHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]