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The map label for this gene is pyrE

Identifier: 154244015

GI number: 154244015

Start: 60356

End: 61054

Strand: Reverse

Name: pyrE

Synonym: Xaut_0057

Alternate gene names: 154244015

Gene position: 61054-60356 (Counterclockwise)

Preceding gene: 154244016

Following gene: 154244014

Centisome position: 1.15

GC content: 66.24

Gene sequence:

>699_bases
ATGGCGAATGTCACTGCTTTTCCGGACAAGGCCACCATCGCCGAGCAGACCGCGCGGATGCTGCTGGAGGTGGAGGCCGT
GCGCTTTTCCTCCGGCGAGCCGTTCATCTTCACCTCCGGCTGGGCCAGCCCGGTCTATATCGACTGCCGGCGCCTCATCT
CCTTCCCCCGCGTGCGCCAGACGCTGGTGGACTTCGGCATCTCCACCATCTTCCGCGAGATCGGCTACGAGCAGTTCGAT
ACCGTAGCCGGCGGCGAGACGGCGGGCATCCCCTTCGCAGCCTGGGTGGCGGACCGGCTGATGTTGCCCATGCAGTATGT
GCGCAAGAAGCCCAAGGGCTTCGGGCGCAACGCACAGATCGAGGGACACCTGGCCCCCGGCCAGCGGGTGCTGCTGGTGG
AGGACCTCACCACCGACGGCAAGAGCAAGGTGACCTTCGTCAACGCCCTGCGCGAGGCCGGGGCGGAGTGCTCGCACTGT
TTCGTGTTCTTCTATTACGACATCTTCGCCGAGGCCTCGGGCATCTTCGACCAGGCCGGGCTCAGCCTGCACCGCCTCGC
CACCTGGTGGGACGTGCTGGCGGCGGTGAAGACCATGAACCGGTTCGAATCCGCCCAGATCGCCGAGATCGAGTCCTTCC
TCCACGATCCCCACTCCTGGTCGAAGGCCCACGGCGGCACCGCCGTCACCGGCGAGTGA

Upstream 100 bases:

>100_bases
GCCTGCGGCTCATTTGCGTGCCTGCGGCGCCTGGGCTGTCGCATTAGGCCACGGCTCTGATATATGCGCGTCACATAATC
GAGGCACCGAGGCCCTTCCC

Downstream 100 bases:

>100_bases
CCGCGCGGATGGACCTCTACGGCGTCCTCAAGCCCCTGTTCCGGTTCATGACGCCGGAGCAGGCGCATCACCTGTCCATC
CGCGTGTTGAAGAGCGGCCT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MANVTAFPDKATIAEQTARMLLEVEAVRFSSGEPFIFTSGWASPVYIDCRRLISFPRVRQTLVDFGISTIFREIGYEQFD
TVAGGETAGIPFAAWVADRLMLPMQYVRKKPKGFGRNAQIEGHLAPGQRVLLVEDLTTDGKSKVTFVNALREAGAECSHC
FVFFYYDIFAEASGIFDQAGLSLHRLATWWDVLAAVKTMNRFESAQIAEIESFLHDPHSWSKAHGGTAVTGE

Sequences:

>Translated_232_residues
MANVTAFPDKATIAEQTARMLLEVEAVRFSSGEPFIFTSGWASPVYIDCRRLISFPRVRQTLVDFGISTIFREIGYEQFD
TVAGGETAGIPFAAWVADRLMLPMQYVRKKPKGFGRNAQIEGHLAPGQRVLLVEDLTTDGKSKVTFVNALREAGAECSHC
FVFFYYDIFAEASGIFDQAGLSLHRLATWWDVLAAVKTMNRFESAQIAEIESFLHDPHSWSKAHGGTAVTGE
>Mature_231_residues
ANVTAFPDKATIAEQTARMLLEVEAVRFSSGEPFIFTSGWASPVYIDCRRLISFPRVRQTLVDFGISTIFREIGYEQFDT
VAGGETAGIPFAAWVADRLMLPMQYVRKKPKGFGRNAQIEGHLAPGQRVLLVEDLTTDGKSKVTFVNALREAGAECSHCF
VFFYYDIFAEASGIFDQAGLSLHRLATWWDVLAAVKTMNRFESAQIAEIESFLHDPHSWSKAHGGTAVTGE

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=210, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508631, Length=189, Percent_Identity=31.7460317460317, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17554964, Length=194, Percent_Identity=30.4123711340206, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17933654, Length=201, Percent_Identity=27.8606965174129, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 25751; Mature: 25619

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVTAFPDKATIAEQTARMLLEVEAVRFSSGEPFIFTSGWASPVYIDCRRLISFPRVRQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHCCHHHHH
TLVDFGISTIFREIGYEQFDTVAGGETAGIPFAAWVADRLMLPMQYVRKKPKGFGRNAQI
HHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
EGHLAPGQRVLLVEDLTTDGKSKVTFVNALREAGAECSHCFVFFYYDIFAEASGIFDQAG
EEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHC
LSLHRLATWWDVLAAVKTMNRFESAQIAEIESFLHDPHSWSKAHGGTAVTGE
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHCCCCEECCCC
>Mature Secondary Structure 
ANVTAFPDKATIAEQTARMLLEVEAVRFSSGEPFIFTSGWASPVYIDCRRLISFPRVRQ
CCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCHHHHHHHHHCCHHHHH
TLVDFGISTIFREIGYEQFDTVAGGETAGIPFAAWVADRLMLPMQYVRKKPKGFGRNAQI
HHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
EGHLAPGQRVLLVEDLTTDGKSKVTFVNALREAGAECSHCFVFFYYDIFAEASGIFDQAG
EEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHC
LSLHRLATWWDVLAAVKTMNRFESAQIAEIESFLHDPHSWSKAHGGTAVTGE
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA