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The map label for this gene is livK [C]

Identifier: 153952635

GI number: 153952635

Start: 692993

End: 694108

Strand: Direct

Name: livK [C]

Synonym: JJD26997_0769

Alternate gene names: 153952635

Gene position: 692993-694108 (Clockwise)

Preceding gene: 153951605

Following gene: 153951623

Centisome position: 37.56

GC content: 31.09

Gene sequence:

>1116_bases
ATGAAAAAACTAACTTTAACTTTAAGTGTTTTAACTATGGTAAATTGCTTATATGCTAAGGATATTAATATAGGAGTAGT
TTTACCTTTAACCGGAACTGTGGCAGCTTATGGGCAAGATGTTTTTAACGGAATTGAACTGGCTAATAAGCTACAGCCAA
AACTTTCAAATGGAGATATTATTAAACTTATCGCAATAGATACAAAAGGAGATAAGCTTGAAACTTTAAACGGAGTTAAT
CGTTTAATAGCAACAGATAAAGTCCTTGGGATTATTGGTGAAGCTACAACTCCAAATACTATTCAAGCTATTTCTATAGC
AGAGAAGAAAAAAATTCCTCTAATAGCTCCAGTGGCATCTGGAGATAAATTGCTAGATAAAAAGAAATATGCAAGTAGAG
TTTGTTTTAAAGATAGTTTTCAAGGAGATAAATTTGCTACTTATGTATCTAAGGATTTAGAACTTAAAAACGCTGTTATC
ATTATAGATCAAAGCAATGTTTATTCTTTAGGTTTGGCAAGAGCCTTTGAAAATTCTTTTAAGAATAATGGTGGAAAAAT
TATAAAAAAACTTGTTATTAATTCAGGAGATAAAGATTTTAGAGCTGTGGTTTCTCAACTTAGGAGTTTAAATCCTGATT
TTGTTTATATGCCTATTTATCATCCTGAAGCTGCTTTAATTGCGAGACAAGCAAGACAAATTGGTTTTGATAAACTTTTA
GCAGCAGGAGATGGTGTTAATAATCAAACTTTTATAGATCTTGGTGGAAGTGCTGTCAATGGAGTAATTTTCACTGATAG
TTTTGATTATAACAATCCATCTACACAATTAGGAAAAGATTTTGTTGCAGCTTATGAGAAAGTTAAAGGAACAAAAGAAC
TTCCAGCATTTTCGGCTATGGGGGCTGATGCGTATTTTGTAATGTTAAATGCTATGAATGCTTGCGTTGATAATCTTAGC
AGCGAATGTATTAATAACAAAATTCATCAAACTAAAGATTTTCAAGCTGTTGGTGGTGTGATTAGCATAGATGAAAGCGG
TAATGCCATTCGTTCTGTTGTGATTAAAGAAATTCAAAATCAAAAACAAAATTATAAAACAATTATAAATCCATAA

Upstream 100 bases:

>100_bases
TTTGCAAGTAAAAGACCTTCTTGAAGAAGAACCTGCGCAAAAAACAAAACATAATATATTTCTAAAATAAAATGTAAAAA
CTATAATTAAAAGGAATGCT

Downstream 100 bases:

>100_bases
TTACTAAAAGAAAGGTAGAGAATGAAAAAAAGTTTAATTTTAGCAAGTATTCTAAGTTTAAGTTTAAGTGCTGCGGAAGT
CAAAATAGGAGTTGTTTTAC

Product: high affinity branched-chain amino acid ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDIIKLIAIDTKGDKLETLNGVN
RLIATDKVLGIIGEATTPNTIQAISIAEKKKIPLIAPVASGDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLELKNAVI
IIDQSNVYSLGLARAFENSFKNNGGKIIKKLVINSGDKDFRAVVSQLRSLNPDFVYMPIYHPEAALIARQARQIGFDKLL
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGKDFVAAYEKVKGTKELPAFSAMGADAYFVMLNAMNACVDNLS
SECINNKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQNQKQNYKTIINP

Sequences:

>Translated_371_residues
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDIIKLIAIDTKGDKLETLNGVN
RLIATDKVLGIIGEATTPNTIQAISIAEKKKIPLIAPVASGDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLELKNAVI
IIDQSNVYSLGLARAFENSFKNNGGKIIKKLVINSGDKDFRAVVSQLRSLNPDFVYMPIYHPEAALIARQARQIGFDKLL
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGKDFVAAYEKVKGTKELPAFSAMGADAYFVMLNAMNACVDNLS
SECINNKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQNQKQNYKTIINP
>Mature_371_residues
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDIIKLIAIDTKGDKLETLNGVN
RLIATDKVLGIIGEATTPNTIQAISIAEKKKIPLIAPVASGDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLELKNAVI
IIDQSNVYSLGLARAFENSFKNNGGKIIKKLVINSGDKDFRAVVSQLRSLNPDFVYMPIYHPEAALIARQARQIGFDKLL
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGKDFVAAYEKVKGTKELPAFSAMGADAYFVMLNAMNACVDNLS
SECINNKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQNQKQNYKTIINP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=274, Percent_Identity=29.1970802919708, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789867, Length=279, Percent_Identity=28.3154121863799, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40267; Mature: 40267

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDI
CCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IKLIAIDTKGDKLETLNGVNRLIATDKVLGIIGEATTPNTIQAISIAEKKKIPLIAPVAS
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCEEEECCC
GDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLELKNAVIIIDQSNVYSLGLARAFENSF
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEECCEEEEEECCCCHHHHHHHHHHHHH
KNNGGKIIKKLVINSGDKDFRAVVSQLRSLNPDFVYMPIYHPEAALIARQARQIGFDKLL
HCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCHHHHH
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGKDFVAAYEKVKGTKELPAFSAM
HCCCCCCCCEEEEECCCEECCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHC
GADAYFVMLNAMNACVDNLSSECINNKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQN
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHCCEEEECCCCHHHHHHHHHHHHH
QKQNYKTIINP
HHHCCCCCCCC
>Mature Secondary Structure
MKKLTLTLSVLTMVNCLYAKDINIGVVLPLTGTVAAYGQDVFNGIELANKLQPKLSNGDI
CCEEEEHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IKLIAIDTKGDKLETLNGVNRLIATDKVLGIIGEATTPNTIQAISIAEKKKIPLIAPVAS
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCCCEEEECCC
GDKLLDKKKYASRVCFKDSFQGDKFATYVSKDLELKNAVIIIDQSNVYSLGLARAFENSF
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEECCEEEEEECCCCHHHHHHHHHHHHH
KNNGGKIIKKLVINSGDKDFRAVVSQLRSLNPDFVYMPIYHPEAALIARQARQIGFDKLL
HCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCHHHHH
AAGDGVNNQTFIDLGGSAVNGVIFTDSFDYNNPSTQLGKDFVAAYEKVKGTKELPAFSAM
HCCCCCCCCEEEEECCCEECCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHC
GADAYFVMLNAMNACVDNLSSECINNKIHQTKDFQAVGGVISIDESGNAIRSVVIKEIQN
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHCCEEEECCCCHHHHHHHHHHHHH
QKQNYKTIINP
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]