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The map label for this gene is cysK [H]

Identifier: 153952497

GI number: 153952497

Start: 801217

End: 802116

Strand: Direct

Name: cysK [H]

Synonym: JJD26997_0902

Alternate gene names: 153952497

Gene position: 801217-802116 (Clockwise)

Preceding gene: 153951444

Following gene: 153952096

Centisome position: 43.42

GC content: 32.56

Gene sequence:

>900_bases
ATGAAAGTACATGAAAAAGTAAGTGAGCTTATAGGAAATACTCCTATTATACATTTGAAAAAATTTGGTATCAATGTTTT
TGCTAAATGTGAATTTTTAAATCCTAGTCATTCTATAAAAGATAGAGCAGCTTTTGAAATGATTAAAGATGCTTTAGATA
GCAAAAAAATTAATCAAAATACTACAATAGTTGAAGCAACTAGTGGAAATACTGGAATTTCTTTGGCTATGATTTGTGCA
GATTTAGGACTTCAATTTGTTGCCACTATGCCAGAATCTATGAGTATAGAAAGACGCAAAATGATAACTCTTTTTGGAGC
ACAACTTGAACTAACTCCTGCAAATTTAGGTATGAAAGGTGCAGTGGATAAAGCTAATGAAATTTTAAAAAATACCCCAA
ATTCTTTCATGCCATCGCAGTTTGATAATTTGGCCAATAAAAATGCACACCGCAAAACTACTGCCTTAGAAATATTAAAA
GATTTGGATAATGAACTTGATATTTTTGTAGCTGGTTTTGGAACTGGCGGTACTATTAGTGGAGTTGGGGAAATACTAAA
AGAAAAATTAGAAAAAGTGCATATTGTTGGAGTCGAGCCTCTAAATTCTCCATTGCTTAGCAAAGGTGAAGCGGGAAGCC
ATAAAATACAAGGAATAGGGGCGAATTTTATACCTGCTATTTTAAATAAAGAAGTTATAGATGAAGTGATCACGGTAAGT
AATGAAGATGCTATAAATACAGCCAAAGAACTTGCCAAAAGTGGTTTAATGGTAGGTATATCAAGTGGAGCAAATGTATT
TGCCGCAAGTATGCTAGCAAAGAAATTTCCAGATAAAAGAATTTTAACTATGTTAAACGATACGGCTGAAAGATACCTTT
CAACTGATCTTTTTGCTTAA

Upstream 100 bases:

>100_bases
TCTTTCTTAATTAATTCAGATATTTTGTATCTTTTATTTACTTTATTTTTTTAAACTTTTATAGAACTATCATTATTAAA
TAAAAAAGGAGATTAAAATT

Downstream 100 bases:

>100_bases
GTATAATTATTGAGAATTTAGAAAAATTCTAAGTTCTCTTAAAATTTCTTGTGGATTTAAATCGCTAATTTCTTTATAAA
AATGACCTTTTTCATCAATA

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKVHEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQNTTIVEATSGNTGISLAMICA
DLGLQFVATMPESMSIERRKMITLFGAQLELTPANLGMKGAVDKANEILKNTPNSFMPSQFDNLANKNAHRKTTALEILK
DLDNELDIFVAGFGTGGTISGVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA

Sequences:

>Translated_299_residues
MKVHEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQNTTIVEATSGNTGISLAMICA
DLGLQFVATMPESMSIERRKMITLFGAQLELTPANLGMKGAVDKANEILKNTPNSFMPSQFDNLANKNAHRKTTALEILK
DLDNELDIFVAGFGTGGTISGVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA
>Mature_299_residues
MKVHEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQNTTIVEATSGNTGISLAMICA
DLGLQFVATMPESMSIERRKMITLFGAQLELTPANLGMKGAVDKANEILKNTPNSFMPSQFDNLANKNAHRKTTALEILK
DLDNELDIFVAGFGTGGTISGVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=300, Percent_Identity=37.6666666666667, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI295821200, Length=300, Percent_Identity=37.6666666666667, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4557415, Length=300, Percent_Identity=37.6666666666667, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1788754, Length=311, Percent_Identity=45.6591639871383, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=38.6666666666667, Blast_Score=194, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17535051, Length=305, Percent_Identity=44.9180327868852, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=43.421052631579, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=39.3333333333333, Blast_Score=208, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=39.7959183673469, Blast_Score=203, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17561720, Length=312, Percent_Identity=39.1025641025641, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=39.2523364485981, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=32.8025477707006, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25147552, Length=311, Percent_Identity=30.8681672025724, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=42.3728813559322, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71996324, Length=328, Percent_Identity=27.1341463414634, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=35.6687898089172, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=34.0490797546012, Blast_Score=132, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24643623, Length=302, Percent_Identity=37.4172185430464, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI20129101, Length=302, Percent_Identity=37.4172185430464, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926
- InterPro:   IPR000634 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32286; Mature: 32286

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00165 DEHYDRATASE_SER_THR ; PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVHEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQN
CCHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
TTIVEATSGNTGISLAMICADLGLQFVATMPESMSIERRKMITLFGAQLELTPANLGMKG
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCEEEEECCCCCCCC
AVDKANEILKNTPNSFMPSQFDNLANKNAHRKTTALEILKDLDNELDIFVAGFGTGGTIS
CHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
GVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCHHHHHHHHHCC
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA
CCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVHEKVSELIGNTPIIHLKKFGINVFAKCEFLNPSHSIKDRAAFEMIKDALDSKKINQN
CCHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
TTIVEATSGNTGISLAMICADLGLQFVATMPESMSIERRKMITLFGAQLELTPANLGMKG
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCEEEEECCCCCCCC
AVDKANEILKNTPNSFMPSQFDNLANKNAHRKTTALEILKDLDNELDIFVAGFGTGGTIS
CHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHH
GVGEILKEKLEKVHIVGVEPLNSPLLSKGEAGSHKIQGIGANFIPAILNKEVIDEVITVS
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCHHHHHHHHHCC
NEDAINTAKELAKSGLMVGISSGANVFAASMLAKKFPDKRILTMLNDTAERYLSTDLFA
CCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9034314; 10688204 [H]