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The map label for this gene is dnaG [H]

Identifier: 153952420

GI number: 153952420

Start: 1760862

End: 1762679

Strand: Direct

Name: dnaG [H]

Synonym: JJD26997_1998

Alternate gene names: 153952420

Gene position: 1760862-1762679 (Clockwise)

Preceding gene: 153951972

Following gene: 153951478

Centisome position: 95.43

GC content: 28.6

Gene sequence:

>1818_bases
ATGATAACTAAAGAAAGTATAGAAAATTTATCCCAAAGACTTAATATTGTTGATATTATAGAAAATTATATTGAAGTAAA
AAAACAAGGCTCTAGTTTTGTGTGTATCTGTCCTTTCCATGCAGATAAAAACCCGTCTATGCATATCAATCCTACTAAAG
GTTTTTATCATTGTTTTGCTTGCAAAGCTGGTGGAGATGCTTTTAAATTTGTGATGGATTATGAAAAATTAAGTTTTGCC
GATGCAGTTGAAAAAGTTGCAAACCTTAGTAATTTTACTTTGAGTTATACAAAAGAAAAACAAGAAAACAAAAAAGAGCT
AAAGTCTATACTACCAAGCTTAAATGCATATTTTAAAGATAATCTCAAACACCATAAAGAAGTTTTAACCTATCTTTACC
AAAGAGCTTTAAATGACAAAGATATAGCCAAATTCGAATTAGGTTTTGCAGGAGCAAGTGAAGATAGTATAAGATTATTG
CAAAATCAAAAAATTCCTTTAGAAGATGCTATGAGTGTTGGAGCTTTAAAAAAAGATGAAAATAATGAATTTTACGCAAG
TTTTATATGGCGTATCACCTTTCCAATCTACGATCACAAAGACTTATTAGTAGGGTTTGGAGGGCGTACTTTAAATCCTA
ATGTACCGGCAAAATATGTAAATTCACCCCAAAATATACTTTTTGATAAAAGTAGAATTTTTTATGCTTTTAACATCGCT
AAAGAAAATATAGCCAAAAAAAAAGAAATAATAGTATGCGAAGGCTACATGGATGCAATAGCTTTTCACAAAGCAGGGTT
TAACAATGCTGTAGCAGTTCTTGGAACAGCATTAACCGAACATCATCTACCCTTAATCCGCCGTTATGATGCAAAAGTGA
TTTTATGTTTTGATAATGATGAAGCAGGATTAAAAGCTGCAACACGATCTACATTTTTATTAAGCACAAACAAAATAGAC
GGTAAAGTGGCAATTTTGCAAGGCGGAAAAGATCCTGCTGAGCTCGTAGCAAAAAATGAAAGCGCAAAGCTCCATGATAT
ACTCGATGAAGGAATAGAGCTTGGTGAATTTTATATCAGACGCTTAATATCCACTCATTCCATAATTTCTGCTCTTGATA
AGCAAAAAGCTTTAGAAACTATACAAAAATTCACCTTTAATCTTGAACCTTTAGTGGCAAATTCTTATACTAATCTAGTT
TCAAATTTATTAAAAGTGGATGAAAAATTTATTGTCTTATCACAAAATTCAAAAAAAACCATACAAACCCCTTTAATTTC
TCAAAATAAATACAATTTTCCTAAAGAAAAAATTCACATTGCAGAATTAGAACTTATAGCTTTTTTAAAACAGCATCCAG
AGATATGTAACGATTTTAAGCAAATTAGCGATAGTGTTTGTTTTAAGCATAAGATTTTATTGGATAAAATTTTAGAAAAA
AAAGGTTATGAAGATAGTGATATTAGAGAATTTGAAAGTAAAAATATTCGAAAAAATTTAAACTATAACGAATTCTTACT
AGGTATTTGCAAGGTTAATTTGGCTTTTTTAAACAATGTAAAAATTAAAAATTCTACACTCGCGTTAAAAAAACAACTTT
TTACGCTTATTGATAAAAATTTAAATCTTGTAATTAAAAATTTAAATACCGAAGAATTAAATAATTTTTTAAAAGAATAT
TTAAGCTTCTTAAAACATGAAAAAAACGAAGAAATTTTACAACAATCATTTAGAAACTTAAGTGGAATTTTTGGTAAAAA
AAACTTTACAGCTTGTGATTTAGGATTTAGCACACAAGATAATGACGAACCTTTTTAA

Upstream 100 bases:

>100_bases
GGTCTCTGTATTCTACAAAGAAAAAATCCATAGTCTCGCTCTAAAAATTTTATGCCAAATTATAGCCAAATCTTTGTATA
ATTTTAACAAGGTTTGTAAA

Downstream 100 bases:

>100_bases
TTTATTTAAATTTCAAGCCTTAATTTACCTTTTTTTAAACACAATCACGCTAAAGTATATAAAAATTTTTAAGGATAAAT
TATGATGCCTTTTAGTGATG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFACKAGGDAFKFVMDYEKLSFA
DAVEKVANLSNFTLSYTKEKQENKKELKSILPSLNAYFKDNLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLL
QNQKIPLEDAMSVGALKKDENNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDNDEAGLKAATRSTFLLSTNKID
GKVAILQGGKDPAELVAKNESAKLHDILDEGIELGEFYIRRLISTHSIISALDKQKALETIQKFTFNLEPLVANSYTNLV
SNLLKVDEKFIVLSQNSKKTIQTPLISQNKYNFPKEKIHIAELELIAFLKQHPEICNDFKQISDSVCFKHKILLDKILEK
KGYEDSDIREFESKNIRKNLNYNEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKNLNLVIKNLNTEELNNFLKEY
LSFLKHEKNEEILQQSFRNLSGIFGKKNFTACDLGFSTQDNDEPF

Sequences:

>Translated_605_residues
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFACKAGGDAFKFVMDYEKLSFA
DAVEKVANLSNFTLSYTKEKQENKKELKSILPSLNAYFKDNLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLL
QNQKIPLEDAMSVGALKKDENNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDNDEAGLKAATRSTFLLSTNKID
GKVAILQGGKDPAELVAKNESAKLHDILDEGIELGEFYIRRLISTHSIISALDKQKALETIQKFTFNLEPLVANSYTNLV
SNLLKVDEKFIVLSQNSKKTIQTPLISQNKYNFPKEKIHIAELELIAFLKQHPEICNDFKQISDSVCFKHKILLDKILEK
KGYEDSDIREFESKNIRKNLNYNEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKNLNLVIKNLNTEELNNFLKEY
LSFLKHEKNEEILQQSFRNLSGIFGKKNFTACDLGFSTQDNDEPF
>Mature_605_residues
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFACKAGGDAFKFVMDYEKLSFA
DAVEKVANLSNFTLSYTKEKQENKKELKSILPSLNAYFKDNLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLL
QNQKIPLEDAMSVGALKKDENNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDNDEAGLKAATRSTFLLSTNKID
GKVAILQGGKDPAELVAKNESAKLHDILDEGIELGEFYIRRLISTHSIISALDKQKALETIQKFTFNLEPLVANSYTNLV
SNLLKVDEKFIVLSQNSKKTIQTPLISQNKYNFPKEKIHIAELELIAFLKQHPEICNDFKQISDSVCFKHKILLDKILEK
KGYEDSDIREFESKNIRKNLNYNEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKNLNLVIKNLNTEELNNFLKEY
LSFLKHEKNEEILQQSFRNLSGIFGKKNFTACDLGFSTQDNDEPF

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=471, Percent_Identity=29.9363057324841, Blast_Score=225, Evalue=8e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69074; Mature: 69074

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEEEEE
CKAGGDAFKFVMDYEKLSFADAVEKVANLSNFTLSYTKEKQENKKELKSILPSLNAYFKD
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
NLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLLQNQKIPLEDAMSVGALKKDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCC
NNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
CCCEEEEEEEEEEECEECCCCEEEECCCCCCCCCCCHHHCCCCHHHEEECCEEEEEEEEH
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDND
HHHHCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHEECCCEEEEEECCC
EAGLKAATRSTFLLSTNKIDGKVAILQGGKDPAELVAKNESAKLHDILDEGIELGEFYIR
CCCCHHHCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RLISTHSIISALDKQKALETIQKFTFNLEPLVANSYTNLVSNLLKVDEKFIVLSQNSKKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
IQTPLISQNKYNFPKEKIHIAELELIAFLKQHPEICNDFKQISDSVCFKHKILLDKILEK
HCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGYEDSDIREFESKNIRKNLNYNEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKN
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCC
LNLVIKNLNTEELNNFLKEYLSFLKHEKNEEILQQSFRNLSGIFGKKNFTACDLGFSTQD
CEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
NDEPF
CCCCC
>Mature Secondary Structure
MITKESIENLSQRLNIVDIIENYIEVKKQGSSFVCICPFHADKNPSMHINPTKGFYHCFA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCEEEEEE
CKAGGDAFKFVMDYEKLSFADAVEKVANLSNFTLSYTKEKQENKKELKSILPSLNAYFKD
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
NLKHHKEVLTYLYQRALNDKDIAKFELGFAGASEDSIRLLQNQKIPLEDAMSVGALKKDE
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCC
NNEFYASFIWRITFPIYDHKDLLVGFGGRTLNPNVPAKYVNSPQNILFDKSRIFYAFNIA
CCCEEEEEEEEEEECEECCCCEEEECCCCCCCCCCCHHHCCCCHHHEEECCEEEEEEEEH
KENIAKKKEIIVCEGYMDAIAFHKAGFNNAVAVLGTALTEHHLPLIRRYDAKVILCFDND
HHHHCCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHEECCCEEEEEECCC
EAGLKAATRSTFLLSTNKIDGKVAILQGGKDPAELVAKNESAKLHDILDEGIELGEFYIR
CCCCHHHCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
RLISTHSIISALDKQKALETIQKFTFNLEPLVANSYTNLVSNLLKVDEKFIVLSQNSKKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCH
IQTPLISQNKYNFPKEKIHIAELELIAFLKQHPEICNDFKQISDSVCFKHKILLDKILEK
HCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGYEDSDIREFESKNIRKNLNYNEFLLGICKVNLAFLNNVKIKNSTLALKKQLFTLIDKN
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCC
LNLVIKNLNTEELNNFLKEYLSFLKHEKNEEILQQSFRNLSGIFGKKNFTACDLGFSTQD
CEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
NDEPF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]