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The map label for this gene is thiH [H]

Identifier: 153952402

GI number: 153952402

Start: 662699

End: 663844

Strand: Direct

Name: thiH [H]

Synonym: JJD26997_0737

Alternate gene names: 153952402

Gene position: 662699-663844 (Clockwise)

Preceding gene: 153951730

Following gene: 153951458

Centisome position: 35.92

GC content: 34.64

Gene sequence:

>1146_bases
ATGCAAGATTATATGCAGCATTTACCTCATATGCAGGAAATAAAAAGCGAAATTTTAAACAAGGTTTTAATTCAAGTTCA
AAGCTATGATGAGAGTCAATTTAGTGCTAAAGATGTTAAAAATGCTTTAAATCAAACGCATTTAAGTATAGAGCATTTAA
AAGCTTTGCTTTCAAGTGCGGCAGAAGATTTCATAGAAGAATTAGCCTTTAAATCCGCTAAAGTCAAGCAAAAATATTTT
GGAAATTCTATCTCTCTTTTTACTCCGCTTTATTTGTCAAATTATTGTAATTCTAAATGTGTTTATTGTGGTTTTCAAAA
AGGCAATAAAATCGCAAGAGCTAAGCTTAGTGAAGTTGAAATTCACGAAGAAATGCAAGCCATTGCTAAAAGTGGTTTAG
AGGAAATTTTAATGCTAACAGGCGAGGGTAGGGAGTTTGCAAGTGTAGAATATATAGCAAATGCCTGCAAGATAGCTAGA
GAGTATTTTAAGGTTGTAGGCGTTGAAATTTATCCTATGAATGAGGAAGAATATAAAATTTTGCATGAAAAGGGTTGTGA
TTATGTAACCGTTTTTCAAGAAACTTATAATCCTTTAAAATATTCTAAAATTCATTTATCTGGTGAAAAACGCATTTTTC
CTTATCGTTTTAACGCTCAAGAAAGAGCTTTAAAAGCGGGTATGCGTGGGGTGGCCTTTGGGGCGCTTTTGGGTATAGAT
GATTTTAGAAAAGACGCGCTTGCTACGGCACTTCATACGCATTTTTTACAACAAACTTATCCACATGCTGAAATTTCTAT
TTCAGTGCCGCGTTTAAGACCTATTATTAATAATGCCAAAATTCATCCTAAAGATGTGAGTGAAAAACGCCTTTTACAAG
TACTTTGTGCTTATAGACTCTTTTTACCTTTTGCGGGCATTACCATTTCAAGCCGTGAAAGAATAGGTTTTAGAGATGAG
GTTATCAAGCTTGGCGCTACTAAAATGAGTGCAGGAGTGAGCGTGGGTATAGGTGAGCATAAGGGCGAGAAAAAGGGTGA
TGGGCAGTTTGAAATTTCAGATGATAGGAGTGTAGATGAAATTTTAGCTATGCTTAAAAAATCAAATTTACAAGCTGTGA
TGAGTGATAGTATCTATGTGGGATAA

Upstream 100 bases:

>100_bases
TGGCAGTAAATGCAGGAAGAGCAGCTTTTTTAGCAGGTTTAGCAAGTGTGAGCGAGGCTAAAGCAAGTTCTCCATTAACA
GGCTTTTTAAGGGATTGATC

Downstream 100 bases:

>100_bases
AAAAATCATCGCCATAAGCGATAGAAAATGTGTAGAAATAGACTTCTTAAAGCAGATTGAAAAACTTGCTAAGGCTAAGG
TAGATGCTATAGTTTTAAGA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MQDYMQHLPHMQEIKSEILNKVLIQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSAAEDFIEELAFKSAKVKQKYF
GNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLSEVEIHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAR
EYFKVVGVEIYPMNEEEYKILHEKGCDYVTVFQETYNPLKYSKIHLSGEKRIFPYRFNAQERALKAGMRGVAFGALLGID
DFRKDALATALHTHFLQQTYPHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRLFLPFAGITISSRERIGFRDE
VIKLGATKMSAGVSVGIGEHKGEKKGDGQFEISDDRSVDEILAMLKKSNLQAVMSDSIYVG

Sequences:

>Translated_381_residues
MQDYMQHLPHMQEIKSEILNKVLIQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSAAEDFIEELAFKSAKVKQKYF
GNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLSEVEIHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAR
EYFKVVGVEIYPMNEEEYKILHEKGCDYVTVFQETYNPLKYSKIHLSGEKRIFPYRFNAQERALKAGMRGVAFGALLGID
DFRKDALATALHTHFLQQTYPHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRLFLPFAGITISSRERIGFRDE
VIKLGATKMSAGVSVGIGEHKGEKKGDGQFEISDDRSVDEILAMLKKSNLQAVMSDSIYVG
>Mature_381_residues
MQDYMQHLPHMQEIKSEILNKVLIQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSAAEDFIEELAFKSAKVKQKYF
GNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLSEVEIHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAR
EYFKVVGVEIYPMNEEEYKILHEKGCDYVTVFQETYNPLKYSKIHLSGEKRIFPYRFNAQERALKAGMRGVAFGALLGID
DFRKDALATALHTHFLQQTYPHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRLFLPFAGITISSRERIGFRDE
VIKLGATKMSAGVSVGIGEHKGEKKGDGQFEISDDRSVDEILAMLKKSNLQAVMSDSIYVG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=350, Percent_Identity=37.1428571428571, Blast_Score=242, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43069; Mature: 43069

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDYMQHLPHMQEIKSEILNKVLIQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSA
CCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEDFIEELAFKSAKVKQKYFGNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLSEVE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
IHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAREYFKVVGVEIYPMNEEEYKI
HHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHH
LHEKGCDYVTVFQETYNPLKYSKIHLSGEKRIFPYRFNAQERALKAGMRGVAFGALLGID
HHHCCCCEEEEEHHHCCCCEEEEEEECCCCEEEEEECCHHHHHHHHCCHHHHHHHHHCHH
DFRKDALATALHTHFLQQTYPHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
FLPFAGITISSRERIGFRDEVIKLGATKMSAGVSVGIGEHKGEKKGDGQFEISDDRSVDE
HHHHCCCEECCCCCCCHHHHHHHHCCHHHHCCEEECCCCCCCCCCCCCCEEECCCCCHHH
ILAMLKKSNLQAVMSDSIYVG
HHHHHHHCCHHHHHCCCEECC
>Mature Secondary Structure
MQDYMQHLPHMQEIKSEILNKVLIQVQSYDESQFSAKDVKNALNQTHLSIEHLKALLSSA
CCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEDFIEELAFKSAKVKQKYFGNSISLFTPLYLSNYCNSKCVYCGFQKGNKIARAKLSEVE
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHH
IHEEMQAIAKSGLEEILMLTGEGREFASVEYIANACKIAREYFKVVGVEIYPMNEEEYKI
HHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHH
LHEKGCDYVTVFQETYNPLKYSKIHLSGEKRIFPYRFNAQERALKAGMRGVAFGALLGID
HHHCCCCEEEEEHHHCCCCEEEEEEECCCCEEEEEECCHHHHHHHHCCHHHHHHHHHCHH
DFRKDALATALHTHFLQQTYPHAEISISVPRLRPIINNAKIHPKDVSEKRLLQVLCAYRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
FLPFAGITISSRERIGFRDEVIKLGATKMSAGVSVGIGEHKGEKKGDGQFEISDDRSVDE
HHHHCCCEECCCCCCCHHHHHHHHCCHHHHCCEEECCCCCCCCCCCCCCEEECCCCCHHH
ILAMLKKSNLQAVMSDSIYVG
HHHHHHHCCHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]