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The map label for this gene is pepT [C]

Identifier: 153952270

GI number: 153952270

Start: 724018

End: 725238

Strand: Direct

Name: pepT [C]

Synonym: JJD26997_0801

Alternate gene names: 153952270

Gene position: 724018-725238 (Clockwise)

Preceding gene: 153950986

Following gene: 153951823

Centisome position: 39.24

GC content: 31.37

Gene sequence:

>1221_bases
ATGGATTATAAGCAAATACAAATTGATAATTTTTTCAGATATCTAGCTGTGCCTTCACAAAGCAATGCTAAAGCACAAAG
CTTGCCAAGCACGCAAGGGCAATATGATTTAGCTCTTTTATTAAAAAAAGAACTTGAAGATCTAGGTTTAAGCGATATAG
TGCTTGAAGATAATGCTATATTAAGTGGCAAACTTAAGGGAAATACCAATAAAAAAAGCATAGGCTTTTGTGCTCATTTA
GACACTGTGGATGTAGGTTTAAGCGAGGTTATTAAGCCTCAAATTTTAAAATTTGAAGGAAAAGCTTTGCATCTTAATAA
GGAAAAAAATATATTTTGTGATCCTAAAGAGCATCCTGAGCTTTTAAAATATCTTGATGAAGATATTATTTTTTCAGATG
GAACAAGTGTTTTGGGTGCAGATAATAAAGCTGCTATAGCAACCATTATGACTTTACTTGTTTATTTAGTGAATGAAAAA
TGCGATCATGGCGATGTTTATGTGTGTTTTGTTCCTGATGAAGAAATAGGACTTCGTGGATCTAAGGCTCTTGATTTAAA
ATATTTTAATCCTGATTTTGCTTTTACTATTGATTGTTGTGAGCTTGGAGAATTTGTTTTTGAAACTTTTAATGCGGGTT
CTGCCTTTATAGAGATAGAAGGAGTTAGTGCTCATCCTATGAGTGCTAAGGGCGTGCTTTTAAATCCTACTTTAATAGGT
CTTGATTTGGCAAATTGTTTTGATAGATTGCAAACTCCTGAGAATACTCAAGATGATGAGGGTTATATTTGGGTTCAAGA
ATTTCATTCTAATCAAATCAAAGCCAGTATGCTTTTAAACATTAGAGATCACAATAAAGAAAAATATGAAAAGAAAAAAG
CTTATATTAGAGAAGTGGTCGATTTTATACAAAAGCGTTATGAGAAGGCTAAGATTTCTTTAAGATTAGAAGATACTTAT
TCCAATCTTAAAGACAGCATAAATGAAAAAAATAAAATTGCTTTGGATATTTTAAAACAAGCTTTTAAAGAATGTCAAAT
AGAGCCAAAAATTTTTCCCATGAGAGGAGGAACTGATGGCAGTGCTTTGGCTTTAAAGGGTTTATTTACGCCTAATTTTT
TTACAGGAGCACATAATTTTCATTCTGCTTTTGAATTTTTACCTTTAAAATCTTTTTATAAAAGTTTTGAAGTGACTAAG
ACCATAGCTTTGTTGGCTTAA

Upstream 100 bases:

>100_bases
CTTAATGATAGCTTTGACTTTAACCTTATATTTATTTTGGATTTTTAATATACCTTTGGGTTTTGAAAGTTCTTATATTT
ACACTTTGAAAGGATAAAAA

Downstream 100 bases:

>100_bases
ATTTTCTAAAAGTGACATTTTGTTATATGAGTTACTTTTTTTAGATCAATTTTTCTCTTTTTTTAGATTTTTAGTTATAT
TTATAAAAAGGAGGGGTTAT

Product: peptidase T

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MDYKQIQIDNFFRYLAVPSQSNAKAQSLPSTQGQYDLALLLKKELEDLGLSDIVLEDNAILSGKLKGNTNKKSIGFCAHL
DTVDVGLSEVIKPQILKFEGKALHLNKEKNIFCDPKEHPELLKYLDEDIIFSDGTSVLGADNKAAIATIMTLLVYLVNEK
CDHGDVYVCFVPDEEIGLRGSKALDLKYFNPDFAFTIDCCELGEFVFETFNAGSAFIEIEGVSAHPMSAKGVLLNPTLIG
LDLANCFDRLQTPENTQDDEGYIWVQEFHSNQIKASMLLNIRDHNKEKYEKKKAYIREVVDFIQKRYEKAKISLRLEDTY
SNLKDSINEKNKIALDILKQAFKECQIEPKIFPMRGGTDGSALALKGLFTPNFFTGAHNFHSAFEFLPLKSFYKSFEVTK
TIALLA

Sequences:

>Translated_406_residues
MDYKQIQIDNFFRYLAVPSQSNAKAQSLPSTQGQYDLALLLKKELEDLGLSDIVLEDNAILSGKLKGNTNKKSIGFCAHL
DTVDVGLSEVIKPQILKFEGKALHLNKEKNIFCDPKEHPELLKYLDEDIIFSDGTSVLGADNKAAIATIMTLLVYLVNEK
CDHGDVYVCFVPDEEIGLRGSKALDLKYFNPDFAFTIDCCELGEFVFETFNAGSAFIEIEGVSAHPMSAKGVLLNPTLIG
LDLANCFDRLQTPENTQDDEGYIWVQEFHSNQIKASMLLNIRDHNKEKYEKKKAYIREVVDFIQKRYEKAKISLRLEDTY
SNLKDSINEKNKIALDILKQAFKECQIEPKIFPMRGGTDGSALALKGLFTPNFFTGAHNFHSAFEFLPLKSFYKSFEVTK
TIALLA
>Mature_406_residues
MDYKQIQIDNFFRYLAVPSQSNAKAQSLPSTQGQYDLALLLKKELEDLGLSDIVLEDNAILSGKLKGNTNKKSIGFCAHL
DTVDVGLSEVIKPQILKFEGKALHLNKEKNIFCDPKEHPELLKYLDEDIIFSDGTSVLGADNKAAIATIMTLLVYLVNEK
CDHGDVYVCFVPDEEIGLRGSKALDLKYFNPDFAFTIDCCELGEFVFETFNAGSAFIEIEGVSAHPMSAKGVLLNPTLIG
LDLANCFDRLQTPENTQDDEGYIWVQEFHSNQIKASMLLNIRDHNKEKYEKKKAYIREVVDFIQKRYEKAKISLRLEDTY
SNLKDSINEKNKIALDILKQAFKECQIEPKIFPMRGGTDGSALALKGLFTPNFFTGAHNFHSAFEFLPLKSFYKSFEVTK
TIALLA

Specific function: Releases The N-Terminal Amino Acid From Tripeptides. Has A Preference For Tripeptides Containing A N-Terminal Methionine Or Leucine (By Similarity). [C]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=402, Percent_Identity=36.0696517412935, Blast_Score=261, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.4.11.4 [C]

Molecular weight: Translated: 45731; Mature: 45731

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKQIQIDNFFRYLAVPSQSNAKAQSLPSTQGQYDLALLLKKELEDLGLSDIVLEDNAI
CCCCEEEHHHEEEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCE
LSGKLKGNTNKKSIGFCAHLDTVDVGLSEVIKPQILKFEGKALHLNKEKNIFCDPKEHPE
EEEEECCCCCCCCEEEEEECCHHHCCHHHHCCCHHEEECCCEEEECCCCCEEECCCCCHH
LLKYLDEDIIFSDGTSVLGADNKAAIATIMTLLVYLVNEKCDHGDVYVCFVPDEEIGLRG
HHHHHCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
SKALDLKYFNPDFAFTIDCCELGEFVFETFNAGSAFIEIEGVSAHPMSAKGVLLNPTLIG
CCEEEEEEECCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCEEEE
LDLANCFDRLQTPENTQDDEGYIWVQEFHSNQIKASMLLNIRDHNKEKYEKKKAYIREVV
EEHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEHHCCHHHHHHHHHHHHHHH
DFIQKRYEKAKISLRLEDTYSNLKDSINEKNKIALDILKQAFKECQIEPKIFPMRGGTDG
HHHHHHHHHEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCC
SALALKGLFTPNFFTGAHNFHSAFEFLPLKSFYKSFEVTKTIALLA
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MDYKQIQIDNFFRYLAVPSQSNAKAQSLPSTQGQYDLALLLKKELEDLGLSDIVLEDNAI
CCCCEEEHHHEEEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCE
LSGKLKGNTNKKSIGFCAHLDTVDVGLSEVIKPQILKFEGKALHLNKEKNIFCDPKEHPE
EEEEECCCCCCCCEEEEEECCHHHCCHHHHCCCHHEEECCCEEEECCCCCEEECCCCCHH
LLKYLDEDIIFSDGTSVLGADNKAAIATIMTLLVYLVNEKCDHGDVYVCFVPDEEIGLRG
HHHHHCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCC
SKALDLKYFNPDFAFTIDCCELGEFVFETFNAGSAFIEIEGVSAHPMSAKGVLLNPTLIG
CCEEEEEEECCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCEEEE
LDLANCFDRLQTPENTQDDEGYIWVQEFHSNQIKASMLLNIRDHNKEKYEKKKAYIREVV
EEHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEHHCCHHHHHHHHHHHHHHH
DFIQKRYEKAKISLRLEDTYSNLKDSINEKNKIALDILKQAFKECQIEPKIFPMRGGTDG
HHHHHHHHHEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCC
SALALKGLFTPNFFTGAHNFHSAFEFLPLKSFYKSFEVTKTIALLA
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]