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The map label for this gene is pmrA [H]

Identifier: 153952030

GI number: 153952030

Start: 415944

End: 417128

Strand: Direct

Name: pmrA [H]

Synonym: JJD26997_0468

Alternate gene names: 153952030

Gene position: 415944-417128 (Clockwise)

Preceding gene: 153952166

Following gene: 153952181

Centisome position: 22.54

GC content: 36.46

Gene sequence:

>1185_bases
ATGGAAAATTTCAATAGAACTTTGCTTGTGTGTTGGTTTGGTGTTTTTACCACAAGTATGGGGCTTAGTCAAATCGCTCC
GATTTTGCCTTTTTATATCAAGGAACTAGGACATGTTGATACGAGTGAGATAGCGTTTTACTCAGGACTTGCTTTTGGGA
TCACTCCGCTTTTTATGGCTATTTTTTCACCTTTGTGGGCTTTTTTGGGTGCAAAGTATGGTTATAAAAATATGCTTCTT
CGTGCAAGTTTTGGAATGTCGGTTTTAACCTTATGGCTTAGCTTTGCTCATAGTGCTTTAGAAGTGGTTTTTGTGCGGGG
TTTAACAGGGATAATTTCGGGTTTTACTTCTGCTGCTGTTGTGTTTATCGCTGTGATAGCGCCTAAAGAAAAAGTGGCTT
ATGCTTTAGGAACTTTTTCTACGGCTTCTATAAGTGGAAGTTTGCTTGGACCTTTGTTTGGTGGCTTTGTGGCTGAATTT
TTTAGCATTAGTGCGGTTTTTGATGTGGTGGCTTTTTTGATCGCTTGTTCTTTTGTGACGATTTATTTTTTCATCCATGA
GAGGAAAATTCAAAAAGAGGCGAAGAAAAATACTCAAAAAGTGAAAGAAAACAAAACCTTTATCATCGTTCTTTTTATCA
CTACTTTTGTGATGCAATTTGGTACTTTTGGAGTGATGCCGATTTTAAGTATTTATGTTGAGCAAATTCATCAAGGTGGA
AATTTGGCTCTTTGGGCGGGTATAGTGGTGGCTGCAAGTGGGATTAGCAATCTTTTTTTCGCGCCAAAACTTGGAAAAAT
CGCTGATAAGATAGGGCCTAGTAAGATTATTTTTGGGGCTTTGATATTTTGTGGAATTTGCTTTTACTTGCAGGCTGTAG
CAAGTAATGTTTATACGCTTATTTTCGTGCGTTTGCTTATAGGTATAGGACTTGGAGGGCTTTTGCCTTGTGTGAATGCC
TTGCTTAAAAAAAGTGTGAGTGCTAAAAATTTAAGTGTGATTTTTGGTTTTAATCAAACTTGTCAATTTTTGGGAAATTT
TTGTGGAGCTTTTGGCGGAGGGCTTATGGCTTCGCATTTTAGCGTGGAATTTGTTTTTACTTTTGTGTGTTTGATTTTTA
TCATTAATGCTTTTATTTTCTTAGCGTTTGAGAAAAAATATATCTTTTCAAATCAAGGTTTATAA

Upstream 100 bases:

>100_bases
GGCGAACGGCATATATTGCCTAAGCATATAAAGAGTATTTTTTTCATTTTTATCCTTGAGTTTTAATTAAAAATTATAAA
AAATATCATACAATAAATCC

Downstream 100 bases:

>100_bases
ATGTTAGAAAGGTTTAAAATGGAAATGATTTTATTTGTTTTGGTGATGATTTTGCTTGTGCTTTTATTTATAGTGCTTTT
TAGGCAGTATAAAAGCCCTA

Product: multidrug resistance efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MENFNRTLLVCWFGVFTTSMGLSQIAPILPFYIKELGHVDTSEIAFYSGLAFGITPLFMAIFSPLWAFLGAKYGYKNMLL
RASFGMSVLTLWLSFAHSALEVVFVRGLTGIISGFTSAAVVFIAVIAPKEKVAYALGTFSTASISGSLLGPLFGGFVAEF
FSISAVFDVVAFLIACSFVTIYFFIHERKIQKEAKKNTQKVKENKTFIIVLFITTFVMQFGTFGVMPILSIYVEQIHQGG
NLALWAGIVVAASGISNLFFAPKLGKIADKIGPSKIIFGALIFCGICFYLQAVASNVYTLIFVRLLIGIGLGGLLPCVNA
LLKKSVSAKNLSVIFGFNQTCQFLGNFCGAFGGGLMASHFSVEFVFTFVCLIFIINAFIFLAFEKKYIFSNQGL

Sequences:

>Translated_394_residues
MENFNRTLLVCWFGVFTTSMGLSQIAPILPFYIKELGHVDTSEIAFYSGLAFGITPLFMAIFSPLWAFLGAKYGYKNMLL
RASFGMSVLTLWLSFAHSALEVVFVRGLTGIISGFTSAAVVFIAVIAPKEKVAYALGTFSTASISGSLLGPLFGGFVAEF
FSISAVFDVVAFLIACSFVTIYFFIHERKIQKEAKKNTQKVKENKTFIIVLFITTFVMQFGTFGVMPILSIYVEQIHQGG
NLALWAGIVVAASGISNLFFAPKLGKIADKIGPSKIIFGALIFCGICFYLQAVASNVYTLIFVRLLIGIGLGGLLPCVNA
LLKKSVSAKNLSVIFGFNQTCQFLGNFCGAFGGGLMASHFSVEFVFTFVCLIFIINAFIFLAFEKKYIFSNQGL
>Mature_394_residues
MENFNRTLLVCWFGVFTTSMGLSQIAPILPFYIKELGHVDTSEIAFYSGLAFGITPLFMAIFSPLWAFLGAKYGYKNMLL
RASFGMSVLTLWLSFAHSALEVVFVRGLTGIISGFTSAAVVFIAVIAPKEKVAYALGTFSTASISGSLLGPLFGGFVAEF
FSISAVFDVVAFLIACSFVTIYFFIHERKIQKEAKKNTQKVKENKTFIIVLFITTFVMQFGTFGVMPILSIYVEQIHQGG
NLALWAGIVVAASGISNLFFAPKLGKIADKIGPSKIIFGALIFCGICFYLQAVASNVYTLIFVRLLIGIGLGGLLPCVNA
LLKKSVSAKNLSVIFGFNQTCQFLGNFCGAFGGGLMASHFSVEFVFTFVCLIFIINAFIFLAFEKKYIFSNQGL

Specific function: Efflux pump for various substrates [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=395, Percent_Identity=34.6835443037975, Blast_Score=219, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 42952; Mature: 42952

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9869592; 11463916 [H]