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The map label for this gene is metK [H]

Identifier: 153951903

GI number: 153951903

Start: 566046

End: 567242

Strand: Direct

Name: metK [H]

Synonym: JJD26997_0626

Alternate gene names: 153951903

Gene position: 566046-567242 (Clockwise)

Preceding gene: 153951158

Following gene: 153952484

Centisome position: 30.68

GC content: 35.42

Gene sequence:

>1197_bases
ATGTATCTATTCACTTCAGAAGTCGTAAGTGCAGGTCATCCAGACAAGTGCGCTGATATAATCGCTGATACGATAGTGGA
TATACTCTTGAAAAATGACAAAAATTCAAGAGTGGCGAGTGAGGTTTTTGTCGCAGGAAATAAGGTTGTGATAGGAGGCG
AAGTTAAGTCAAATCATAAACTTAGTAAGACTGATTACGATAATTTAGTTAAAGATGTTTTAAAAAATATTGGTTATGAC
GGAGCAGGGCATTTTAGTAAAGAGCAGTGTTTACATCCTGATGAAGTTGATGTTATGGTGTTTTTAAATGAACAAAGCCC
TGATATCAACCAAGGCGTTGATCAAGAAGATGGCGAAATAGGGGCAGGAGATCAAGGTATTATGTTTGGTTTTGCAAGTT
GCGAGGCTGAAGAATACATGCCAGCAGCTATCAGTTATGCAAGAATGTTGTGTGATAGAGTTTATGCTTATGCAAAAGCA
AATCCACATGAACTTGGAGTAGATATTAAAACTCAAGTAACCATTGATTATGGTACTAAGGCAAATTTTGAAAATTGCAA
ACCGCAAAGCATTCATACTATAGTAGTTTCTGCTCCTTGTGTTGAAAGTATGAACATAGAAGATTTAAGATCTTTGGTGA
TGAAACTTATTTTAGATAGTAACTTGCCAAAAGAACTTTTTGATCCTAATGAAACTAGAATTTTAATCAATCCAACCGGT
AAATATGTCAATCACTCTTCTTTGCATGATAGTGGTTTAACAGGAAGAAAGCTTATAGTAGATAGTTTTGGAGGTTATTC
TCCAATAGGCGGTGGTGCACAATCGAGTAAAGATTATACTAAAGTTGATAGAAGCGGACTTTATGCAGGTAGATGGCTAG
CTAAAAACATAGTTGCAGCAGGACTTGCTAAAAAATGCATCGTTCAACTTAGCTATGCTATAGGTGTTGCAAAACCTACT
TCGGTAAGCGTGGATTGTATGGGTACAAATACTAGTGTAAATGATGATGTTTTAAGTGATTTTGTAATGCAAAATTTCTC
TTTAACACCAAATTGGATTCGTGATAAATTTCATTTAGATAAACCAAGTAAAGAAATTTTTCTTTATGCGGATGTAGCAG
CTCGCGGACAAGTAGGACAAAAAGATTATCCTTGGGAAAAACTTGATGCTTTAGAGCAATTTAAGGCTTTGATGTAA

Upstream 100 bases:

>100_bases
TAACTAACTTTTGCTTAATTTTAGAGAATTTTTAATTTATTAAGCTTTGATAAGTTTATTTCGGTACAATTGTGGTTCAT
TTTAAATGAAAGGACTTTTT

Downstream 100 bases:

>100_bases
ATTTTTAGCGGGAAATCAAATCCCGCTAACTCCCTTTTTTAACTCTAGTTTTTTAAATTTAAAACCCTTCTATTTTTTTT
TTTTTTTTTTGATATAATCC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHKLSKTDYDNLVKDVLKNIGYD
GAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGEIGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKA
NPHELGVDIKTQVTIDYGTKANFENCKPQSIHTIVVSAPCVESMNIEDLRSLVMKLILDSNLPKELFDPNETRILINPTG
KYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAAGLAKKCIVQLSYAIGVAKPT
SVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLDKPSKEIFLYADVAARGQVGQKDYPWEKLDALEQFKALM

Sequences:

>Translated_398_residues
MYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHKLSKTDYDNLVKDVLKNIGYD
GAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGEIGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKA
NPHELGVDIKTQVTIDYGTKANFENCKPQSIHTIVVSAPCVESMNIEDLRSLVMKLILDSNLPKELFDPNETRILINPTG
KYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAAGLAKKCIVQLSYAIGVAKPT
SVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLDKPSKEIFLYADVAARGQVGQKDYPWEKLDALEQFKALM
>Mature_398_residues
MYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHKLSKTDYDNLVKDVLKNIGYD
GAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGEIGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKA
NPHELGVDIKTQVTIDYGTKANFENCKPQSIHTIVVSAPCVESMNIEDLRSLVMKLILDSNLPKELFDPNETRILINPTG
KYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAAGLAKKCIVQLSYAIGVAKPT
SVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLDKPSKEIFLYADVAARGQVGQKDYPWEKLDALEQFKALM

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=390, Percent_Identity=40.5128205128205, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=39.0745501285347, Blast_Score=245, Evalue=6e-65,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=40.6565656565657, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI25145633, Length=395, Percent_Identity=40.7594936708861, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17538498, Length=390, Percent_Identity=40.2564102564103, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17538494, Length=395, Percent_Identity=40, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=40.1496259351621, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=39.6501457725948, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=40.2298850574713, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI32565905, Length=394, Percent_Identity=36.8020304568528, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI32565903, Length=313, Percent_Identity=39.6166134185303, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=41.4634146341463, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=396, Percent_Identity=41.9191919191919, Blast_Score=268, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6320710, Length=396, Percent_Identity=40.9090909090909, Blast_Score=259, Evalue=6e-70,
Organism=Drosophila melanogaster, GI45552159, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24580529, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24580537, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24580533, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24580535, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24580531, Length=395, Percent_Identity=40.5063291139241, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=39.8477157360406, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=39.8477157360406, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=38.8451443569554, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24580539, Length=135, Percent_Identity=36.2962962962963, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43657; Mature: 43657

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHK
CEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEECCCCCCCCC
LSKTDYDNLVKDVLKNIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGEI
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKANPHELGVDIKTQVTIDYGTK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEEEEECCCC
ANFENCKPQSIHTIVVSAPCVESMNIEDLRSLVMKLILDSNLPKELFDPNETRILINPTG
CCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCC
KYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAA
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
GLAKKCIVQLSYAIGVAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLD
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCC
KPSKEIFLYADVAARGQVGQKDYPWEKLDALEQFKALM
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHK
CEEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEECCCCCCCCC
LSKTDYDNLVKDVLKNIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGEI
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
GAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKANPHELGVDIKTQVTIDYGTK
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEEEEECCCC
ANFENCKPQSIHTIVVSAPCVESMNIEDLRSLVMKLILDSNLPKELFDPNETRILINPTG
CCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCC
KYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAA
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
GLAKKCIVQLSYAIGVAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLD
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCC
KPSKEIFLYADVAARGQVGQKDYPWEKLDALEQFKALM
CCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA