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The map label for this gene is thiF [H]

Identifier: 153951833

GI number: 153951833

Start: 661114

End: 661917

Strand: Direct

Name: thiF [H]

Synonym: JJD26997_0735

Alternate gene names: 153951833

Gene position: 661114-661917 (Clockwise)

Preceding gene: 153951064

Following gene: 153951730

Centisome position: 35.83

GC content: 34.45

Gene sequence:

>804_bases
ATGATGAGAGTGAAATTCAATGGCAAAGAACTTGATACAGACTTTAAAACAAGTTTGGAATTTTTTGAAAATATCAGCAA
AAATGAAAATGATGTTTGGATTATCAATGGTTTTGCAACAAAAGAAAATATCGCTTTAAATGAAGATGATGAGCTTTTTT
GTATAGAAAGAAATACTTTGCCTCCAAAAGATGCACTAGATGCTATGATGAGAGCAAGACACACTCCAAAACTTCACGAC
AAGCTTAAAAATGGGCGCGTGGCGGTGTGTGGTTTAGGCGGACTTGGATCGCATATTGTGATAAATTTAGCAAGAAGTGG
AGTGGGGTATTTAAAGCTGATTGATTTTGATGTGATTGAGCCTAGTAATTTGAATCGTCAAGCTTACCGTGTAAGTGATT
TGGGTAAATTTAAAACTGAAGCTTTAAAAGAACAAATTAGTGAAATTAATCCTTATATCAGCGTTGAAATTTGCACTTTA
AAAATCGATGAAGATAATTTAAAGTCTTTGTTTAAAGATATAGATATAGTTTGTGAAGCCTTTGATAGTGCTATCGCAAA
AGCGATGATGGCGCAAAATTTTCATAGATTTTACAAGGATAGTATTTTAATCTGTGCTTCAGGGCTTGCGGGCTATGGTG
ATAACAACAGCATACAAACAAGAAAAATTGCTAAAAATTTCTATGTGTGTGGGGATTTGGTAAATGGTGCTAAGGTGGGA
AATGGACTTATGGCACCAAGGGTAAATATTTGTGCGGGACATCAAAGTAACCTTGTTTTAGAGCTTTTAGCAAATAAGGA
GTAA

Upstream 100 bases:

>100_bases
CTTTAGAATTAAATGGAGAAATCATACCGAAAAGTGAATTTGAAAATTTGATTTTAAAAGAAAATGATAAAGCCGAAATC
GTAAGTTTTGTAGGGGGTGG

Downstream 100 bases:

>100_bases
AAATGCAAGAAAATTTAAAAAATGATAAATTAAAAATAGGCAAATACGAGTTTGATTCAAGATTTATTTTAGGTTCTGGA
AAATATTCGCTAGAACTTAT

Product: thiamine biosynthesis protein ThiF

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MMRVKFNGKELDTDFKTSLEFFENISKNENDVWIINGFATKENIALNEDDELFCIERNTLPPKDALDAMMRARHTPKLHD
KLKNGRVAVCGLGGLGSHIVINLARSGVGYLKLIDFDVIEPSNLNRQAYRVSDLGKFKTEALKEQISEINPYISVEICTL
KIDEDNLKSLFKDIDIVCEAFDSAIAKAMMAQNFHRFYKDSILICASGLAGYGDNNSIQTRKIAKNFYVCGDLVNGAKVG
NGLMAPRVNICAGHQSNLVLELLANKE

Sequences:

>Translated_267_residues
MMRVKFNGKELDTDFKTSLEFFENISKNENDVWIINGFATKENIALNEDDELFCIERNTLPPKDALDAMMRARHTPKLHD
KLKNGRVAVCGLGGLGSHIVINLARSGVGYLKLIDFDVIEPSNLNRQAYRVSDLGKFKTEALKEQISEINPYISVEICTL
KIDEDNLKSLFKDIDIVCEAFDSAIAKAMMAQNFHRFYKDSILICASGLAGYGDNNSIQTRKIAKNFYVCGDLVNGAKVG
NGLMAPRVNICAGHQSNLVLELLANKE
>Mature_267_residues
MMRVKFNGKELDTDFKTSLEFFENISKNENDVWIINGFATKENIALNEDDELFCIERNTLPPKDALDAMMRARHTPKLHD
KLKNGRVAVCGLGGLGSHIVINLARSGVGYLKLIDFDVIEPSNLNRQAYRVSDLGKFKTEALKEQISEINPYISVEICTL
KIDEDNLKSLFKDIDIVCEAFDSAIAKAMMAQNFHRFYKDSILICASGLAGYGDNNSIQTRKIAKNFYVCGDLVNGAKVG
NGLMAPRVNICAGHQSNLVLELLANKE

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787048, Length=192, Percent_Identity=28.125, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789177, Length=146, Percent_Identity=31.5068493150685, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082356, Length=153, Percent_Identity=28.1045751633987, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=110, Percent_Identity=34.5454545454545, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=112, Percent_Identity=34.8214285714286, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582879, Length=112, Percent_Identity=31.25, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 29770; Mature: 29770

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRVKFNGKELDTDFKTSLEFFENISKNENDVWIINGFATKENIALNEDDELFCIERNTL
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCEEEEECCCC
PPKDALDAMMRARHTPKLHDKLKNGRVAVCGLGGLGSHIVINLARSGVGYLKLIDFDVIE
CCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCHHEEEEHHCCCCEEEEEEEEEEC
PSNLNRQAYRVSDLGKFKTEALKEQISEINPYISVEICTLKIDEDNLKSLFKDIDIVCEA
CCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHH
FDSAIAKAMMAQNFHRFYKDSILICASGLAGYGDNNSIQTRKIAKNFYVCGDLVNGAKVG
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEHHHHHHHHEEEECCCCCCCCC
NGLMAPRVNICAGHQSNLVLELLANKE
CCCCCCCEEEECCCCCCEEEEEECCCC
>Mature Secondary Structure
MMRVKFNGKELDTDFKTSLEFFENISKNENDVWIINGFATKENIALNEDDELFCIERNTL
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCEEEEECCCC
PPKDALDAMMRARHTPKLHDKLKNGRVAVCGLGGLGSHIVINLARSGVGYLKLIDFDVIE
CCHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCHHEEEEHHCCCCEEEEEEEEEEC
PSNLNRQAYRVSDLGKFKTEALKEQISEINPYISVEICTLKIDEDNLKSLFKDIDIVCEA
CCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHH
FDSAIAKAMMAQNFHRFYKDSILICASGLAGYGDNNSIQTRKIAKNFYVCGDLVNGAKVG
HHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEHHHHHHHHEEEECCCCCCCCC
NGLMAPRVNICAGHQSNLVLELLANKE
CCCCCCCEEEECCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]