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The map label for this gene is rlmN [H]

Identifier: 153951816

GI number: 153951816

Start: 1829229

End: 1830299

Strand: Direct

Name: rlmN [H]

Synonym: JJD26997_2087

Alternate gene names: 153951816

Gene position: 1829229-1830299 (Clockwise)

Preceding gene: 153951766

Following gene: 153951068

Centisome position: 99.14

GC content: 34.36

Gene sequence:

>1071_bases
TTGAAAGAGCTTGTTAATATTTTAGATTTTTTACCTGAAGAATTAGGAGAAAAAATCAAACCTATGTTTCGCGTGAAACA
AATTTATCAATGGATTTATCAAAAATACGCCAATAATTTTTCCGATATGTCAAGTTTACCAAAAGATCTACGTTTAGAAC
TTGCGCAAAATTTTCATTTTTCTCCTGTAAAATGCGTTAAAAATGAGCAAAGTAAAGATGGAAGTATAAAATATCTTTTT
GAACTCATCGATGGTTTAAGGGTAGAAAGCGTGCTTTTGCCTATGAAAGAAGAAAAAATAGACACAGAAGGCAAAAGAAT
TTCTCATGCGAGATATACTATCTGTGTTTCTTCTCAAGTAGGTTGTAAAAGTAGCTGTAGTTTTTGTCTTACTGCCAAGG
GTGGCTTGAAAAGAAATTTAAGTGCAGGGGAGATAGTCGGACAAATTTTATGGATTAAAAAACAAAACAATATCCCTTAT
GAACGCCGTGTAAATATAGTATATATGGGTATGGGTGAGCCTTTGGATAATCTTAAGAATGTTTCTAAAGCGGTAAAAAT
TTTAGCACAAAACGATGGACTTGCCATAAGTCCCCGCCGTCAAACCATCAGTACTAGTGGTTTGGCAAAACAAATCAAAG
AATTAGGTCAAATGAATTTAGGTGTTTTACTTGCTATATCATTGCACGCGGTTAATGATGAGCTTCGAACTGAGCTTATG
CCTATTAATAAGGCTTACAATATCGCTGCGATTATGGATGCGGTGCGTGAATTTCCTATAGATCAAAGAAAAAGAGTGAT
GTTTGAATATCTTTTGATCGATGGTATCAATGATAAGCTTGAACACGCCAAAGAATTAGTCAAGCTTTTAAATGGCATTA
AAGCTAAAGTGAATTTAATACTCTTTAATCCACATGAAGGAAGTTTATATAAACGCCCTAGTTTAGAAAACGCGATTAAA
TTTCAAGATTTACTCAGTAGCAAAGGTGTAACTTGCACTATAAGAGAAAGTAAAGGGCTTGACATTTCAGCTGCTTGTGG
GCAGCTTAAAGAAAGGGCGAAAGAACAATGA

Upstream 100 bases:

>100_bases
CACGAAGATTTTATAAAAAATCATCAAAAGGCAAAAGAAAAATTAGAAGAATACTTGAAAAAACATCATTATATTTAAAA
ATTCAAAAAGGAAAATAATT

Downstream 100 bases:

>100_bases
CAATGCTAGATATTTTTGAAATCATTTTTATAACAGCTATTGTGATTATAGCTTTTGGTGGAATTGTTTTTGTAGTAACA
AAAGAAAAAAAATAATTATT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHFSPVKCVKNEQSKDGSIKYLF
ELIDGLRVESVLLPMKEEKIDTEGKRISHARYTICVSSQVGCKSSCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPY
ERRVNIVYMGMGEPLDNLKNVSKAVKILAQNDGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLILFNPHEGSLYKRPSLENAIK
FQDLLSSKGVTCTIRESKGLDISAACGQLKERAKEQ

Sequences:

>Translated_356_residues
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHFSPVKCVKNEQSKDGSIKYLF
ELIDGLRVESVLLPMKEEKIDTEGKRISHARYTICVSSQVGCKSSCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPY
ERRVNIVYMGMGEPLDNLKNVSKAVKILAQNDGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLILFNPHEGSLYKRPSLENAIK
FQDLLSSKGVTCTIRESKGLDISAACGQLKERAKEQ
>Mature_356_residues
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHFSPVKCVKNEQSKDGSIKYLF
ELIDGLRVESVLLPMKEEKIDTEGKRISHARYTICVSSQVGCKSSCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPY
ERRVNIVYMGMGEPLDNLKNVSKAVKILAQNDGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLILFNPHEGSLYKRPSLENAIK
FQDLLSSKGVTCTIRESKGLDISAACGQLKERAKEQ

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=357, Percent_Identity=40.0560224089636, Blast_Score=249, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 40246; Mature: 40246

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCC
SPVKCVKNEQSKDGSIKYLFELIDGLRVESVLLPMKEEKIDTEGKRISHARYTICVSSQV
CCHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCEECCCEEEEEECCCC
GCKSSCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPYERRVNIVYMGMGEPLDNLKN
CCCCCCCEEEEECCCCCCCCCHHHHHHHEEEEEECCCCCHHHEEEEEEEECCCCHHHHHH
VSKAVKILAQNDGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCHHHHHHHH
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLI
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
LFNPHEGSLYKRPSLENAIKFQDLLSSKGVTCTIRESKGLDISAACGQLKERAKEQ
EECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKELVNILDFLPEELGEKIKPMFRVKQIYQWIYQKYANNFSDMSSLPKDLRLELAQNFHF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCCCC
SPVKCVKNEQSKDGSIKYLFELIDGLRVESVLLPMKEEKIDTEGKRISHARYTICVSSQV
CCHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCEECCCEEEEEECCCC
GCKSSCSFCLTAKGGLKRNLSAGEIVGQILWIKKQNNIPYERRVNIVYMGMGEPLDNLKN
CCCCCCCEEEEECCCCCCCCCHHHHHHHEEEEEECCCCCHHHEEEEEEEECCCCHHHHHH
VSKAVKILAQNDGLAISPRRQTISTSGLAKQIKELGQMNLGVLLAISLHAVNDELRTELM
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCHHHHHHHH
PINKAYNIAAIMDAVREFPIDQRKRVMFEYLLIDGINDKLEHAKELVKLLNGIKAKVNLI
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEE
LFNPHEGSLYKRPSLENAIKFQDLLSSKGVTCTIRESKGLDISAACGQLKERAKEQ
EECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA