Click here to switch to the map view.

The map label for this gene is mcrB [H]

Identifier: 153951772

GI number: 153951772

Start: 146467

End: 148263

Strand: Direct

Name: mcrB [H]

Synonym: JJD26997_0152

Alternate gene names: 153951772

Gene position: 146467-148263 (Clockwise)

Preceding gene: 153952308

Following gene: 153951881

Centisome position: 7.94

GC content: 25.71

Gene sequence:

>1797_bases
ATGGAAATTATCTTTAAGCAAGATGAAAAAGATAATTTTATTTCAGCAATAAATTTTCTTATTCATGAAGTTGAAACTTG
TATCCAAAGAAATGTTTTAACACAACACGGTAAAGTAAGTGTTAAAGGAACTTCTCAAAAAGATATTAATATCAAGTTAG
ATTTTTTAAAAAAATATAATTATTCTTATAAAATTGGTCTAGGTAATGCAGGTGGATTTTTAAATGAGGATAGTTGGATT
ATTTTTGTTAGAAATGATTTGCTTGCAAGTAATTTGATCAACGGTGAAAAATTAACTGCAACAAAAGGTATTTATATCTT
TATAGCTTTTTCAGGATATTTTTCAGATGATAAACATATACAATTGAGGTTTGGTTTTCCTCAAGGGGATATGAATTTAT
CGAGGTGTAATGCCGTTGATCAAATGGATAGGAGTGGAAAATTACGGTATTTTGATTTTAAGTACAAAGATTTTGAAAAA
GAAACAAATCAAATTGTAAAAGATTTTCAAGATATGATTAATTATTTTAATATTTTTGACAAAAATGATTTTAAATTAAG
AGCTGAATTTTCTACTTTTATACCCTTAAATCAAATTCTTTATGGACCTCCAGGGACAGGAAAAACTTATCATACTATAG
ATAAAGCTTTAGAGATTATATCAAAAGAAGAAAAAATACAAATTCCAAGTGAAGATGATAGAATAAATAGAAAAAAATTA
TTTGATGAGTATGTAAAAAATGGACAAATAGTCTTTACAACTTTTCATCAAAGCTATGGATATGAAGAATTTGTAGAAGG
TATAAAGCCTGATATAAATTCTAAAGAAGTAAAATACAAAATAGAGAATGGAGTTTTTAAAGAATTTTGTGAAAAAGCTT
TGGATAATTATGAGAATAGTATCTTAAATGCAGATGAGTTGAATAAAAAAATAGAATTAAAAGAAAAGTTTGAAAATTTT
TTAAATTGGCTTTTGGAAACAAATGAACCTATTGGTAAAACAAAGGGTGGAAATTTCTTTGTTGTTGATATTAATGATAA
AACAGTTATGATTTATAGCGAAGGAGTAGAGAGATTTGATGGAAATTTTAATTTAAGTATATCTATATTTATGGAGTTAT
TAAAATGCAAAGATGAATTTAACAATGCCGCTGAAATGTTTAAAAAAGTATTTAATAGAGATTATGCCGATAGAACTCAT
ACTTATTACTTTAATTTAGTGAAAAAATTTAAAGCATATGAAAAACAATCAGTTGCAAAGATTGAAAATAATAAAAATAA
TGATAATTCTTTAAAACCTTATGTAATCATCATTGATGAAATCAATCGTGGTAATGTAAGTAAGATTTTTGGTGAGCTTA
TAACCTTGATAGAGCCTAGCAAAAGGATAGGCGTAGAAGAAGAGTTAAAAGTGACTTTGCCTTATAGTAAAAAAGAATTT
GGAGTGCCTAAGAATGTTTATATCATAGGGACTATGAATACAGCCGATAGAAGCATAACTTCTCTTGATACAGCTTTGCG
AAGAAGATTTGAATTTATTGAGATGATGCCTGATGTGAGCAAATTATCTATGGATTGCGAAGGTATTAATTTACAAGAAT
TATTAAAAGCTATAAACACTCGTATAGAATATTTACTAGATAGAGAAAAAACCATAGGTCATGCATTTTTTATAAGTGTT
GAAAATAAAGATTATTTAAAAAATATGACAGAATTTATAGAAAGCGATAAGATTGTTTATAAATTTAGTGATAGTAATAA
TTGGAGTAAAGATACTTTTATAAAAATATATGAATAA

Upstream 100 bases:

>100_bases
TGATGAAAATATCACTTTAGAAAATTTAGAAAATAAAGAAAAAACTACTATAAATTTTAATGATATAAAAAAAGCTAGAA
CCTTTATGGAGTGGTAAGAA

Downstream 100 bases:

>100_bases
TGAAAACAAGTAATACTTTTTCCATCATCGAATACCAAGCTTTTTCTAAAGAAGATTTAAAAGAAATTTTCAAAGAAAAG
GCCGAAGGGTTTTATAAAGA

Product: putative McrBC restriction endonuclease system, McrB subunit

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MEIIFKQDEKDNFISAINFLIHEVETCIQRNVLTQHGKVSVKGTSQKDINIKLDFLKKYNYSYKIGLGNAGGFLNEDSWI
IFVRNDLLASNLINGEKLTATKGIYIFIAFSGYFSDDKHIQLRFGFPQGDMNLSRCNAVDQMDRSGKLRYFDFKYKDFEK
ETNQIVKDFQDMINYFNIFDKNDFKLRAEFSTFIPLNQILYGPPGTGKTYHTIDKALEIISKEEKIQIPSEDDRINRKKL
FDEYVKNGQIVFTTFHQSYGYEEFVEGIKPDINSKEVKYKIENGVFKEFCEKALDNYENSILNADELNKKIELKEKFENF
LNWLLETNEPIGKTKGGNFFVVDINDKTVMIYSEGVERFDGNFNLSISIFMELLKCKDEFNNAAEMFKKVFNRDYADRTH
TYYFNLVKKFKAYEKQSVAKIENNKNNDNSLKPYVIIIDEINRGNVSKIFGELITLIEPSKRIGVEEELKVTLPYSKKEF
GVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINTRIEYLLDREKTIGHAFFISV
ENKDYLKNMTEFIESDKIVYKFSDSNNWSKDTFIKIYE

Sequences:

>Translated_598_residues
MEIIFKQDEKDNFISAINFLIHEVETCIQRNVLTQHGKVSVKGTSQKDINIKLDFLKKYNYSYKIGLGNAGGFLNEDSWI
IFVRNDLLASNLINGEKLTATKGIYIFIAFSGYFSDDKHIQLRFGFPQGDMNLSRCNAVDQMDRSGKLRYFDFKYKDFEK
ETNQIVKDFQDMINYFNIFDKNDFKLRAEFSTFIPLNQILYGPPGTGKTYHTIDKALEIISKEEKIQIPSEDDRINRKKL
FDEYVKNGQIVFTTFHQSYGYEEFVEGIKPDINSKEVKYKIENGVFKEFCEKALDNYENSILNADELNKKIELKEKFENF
LNWLLETNEPIGKTKGGNFFVVDINDKTVMIYSEGVERFDGNFNLSISIFMELLKCKDEFNNAAEMFKKVFNRDYADRTH
TYYFNLVKKFKAYEKQSVAKIENNKNNDNSLKPYVIIIDEINRGNVSKIFGELITLIEPSKRIGVEEELKVTLPYSKKEF
GVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINTRIEYLLDREKTIGHAFFISV
ENKDYLKNMTEFIESDKIVYKFSDSNNWSKDTFIKIYE
>Mature_598_residues
MEIIFKQDEKDNFISAINFLIHEVETCIQRNVLTQHGKVSVKGTSQKDINIKLDFLKKYNYSYKIGLGNAGGFLNEDSWI
IFVRNDLLASNLINGEKLTATKGIYIFIAFSGYFSDDKHIQLRFGFPQGDMNLSRCNAVDQMDRSGKLRYFDFKYKDFEK
ETNQIVKDFQDMINYFNIFDKNDFKLRAEFSTFIPLNQILYGPPGTGKTYHTIDKALEIISKEEKIQIPSEDDRINRKKL
FDEYVKNGQIVFTTFHQSYGYEEFVEGIKPDINSKEVKYKIENGVFKEFCEKALDNYENSILNADELNKKIELKEKFENF
LNWLLETNEPIGKTKGGNFFVVDINDKTVMIYSEGVERFDGNFNLSISIFMELLKCKDEFNNAAEMFKKVFNRDYADRTH
TYYFNLVKKFKAYEKQSVAKIENNKNNDNSLKPYVIIIDEINRGNVSKIFGELITLIEPSKRIGVEEELKVTLPYSKKEF
GVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINTRIEYLLDREKTIGHAFFISV
ENKDYLKNMTEFIESDKIVYKFSDSNNWSKDTFIKIYE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=144, Percent_Identity=40.9722222222222, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 69898; Mature: 69898

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIFKQDEKDNFISAINFLIHEVETCIQRNVLTQHGKVSVKGTSQKDINIKLDFLKKYN
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEHHCC
YSYKIGLGNAGGFLNEDSWIIFVRNDLLASNLINGEKLTATKGIYIFIAFSGYFSDDKHI
CEEEEECCCCCCCCCCCCEEEEEECCHHHHHCCCCCEEEEECCEEEEEEEECCCCCCCEE
QLRFGFPQGDMNLSRCNAVDQMDRSGKLRYFDFKYKDFEKETNQIVKDFQDMINYFNIFD
EEEECCCCCCCCHHHHCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
KNDFKLRAEFSTFIPLNQILYGPPGTGKTYHTIDKALEIISKEEKIQIPSEDDRINRKKL
CCCCEEEEEEECCCCHHHHEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHH
FDEYVKNGQIVFTTFHQSYGYEEFVEGIKPDINSKEVKYKIENGVFKEFCEKALDNYENS
HHHHHHCCEEEEEEECHHCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
ILNADELNKKIELKEKFENFLNWLLETNEPIGKTKGGNFFVVDINDKTVMIYSEGVERFD
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHCC
GNFNLSISIFMELLKCKDEFNNAAEMFKKVFNRDYADRTHTYYFNLVKKFKAYEKQSVAK
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IENNKNNDNSLKPYVIIIDEINRGNVSKIFGELITLIEPSKRIGVEEELKVTLPYSKKEF
HHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCEEEECCCCCHHC
GVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINT
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHH
RIEYLLDREKTIGHAFFISVENKDYLKNMTEFIESDKIVYKFSDSNNWSKDTFIKIYE
HHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MEIIFKQDEKDNFISAINFLIHEVETCIQRNVLTQHGKVSVKGTSQKDINIKLDFLKKYN
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEHHCC
YSYKIGLGNAGGFLNEDSWIIFVRNDLLASNLINGEKLTATKGIYIFIAFSGYFSDDKHI
CEEEEECCCCCCCCCCCCEEEEEECCHHHHHCCCCCEEEEECCEEEEEEEECCCCCCCEE
QLRFGFPQGDMNLSRCNAVDQMDRSGKLRYFDFKYKDFEKETNQIVKDFQDMINYFNIFD
EEEECCCCCCCCHHHHCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
KNDFKLRAEFSTFIPLNQILYGPPGTGKTYHTIDKALEIISKEEKIQIPSEDDRINRKKL
CCCCEEEEEEECCCCHHHHEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHH
FDEYVKNGQIVFTTFHQSYGYEEFVEGIKPDINSKEVKYKIENGVFKEFCEKALDNYENS
HHHHHHCCEEEEEEECHHCCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
ILNADELNKKIELKEKFENFLNWLLETNEPIGKTKGGNFFVVDINDKTVMIYSEGVERFD
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHCC
GNFNLSISIFMELLKCKDEFNNAAEMFKKVFNRDYADRTHTYYFNLVKKFKAYEKQSVAK
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IENNKNNDNSLKPYVIIIDEINRGNVSKIFGELITLIEPSKRIGVEEELKVTLPYSKKEF
HHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHCCCCCCEEEECCCCCHHC
GVPKNVYIIGTMNTADRSITSLDTALRRRFEFIEMMPDVSKLSMDCEGINLQELLKAINT
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHH
RIEYLLDREKTIGHAFFISVENKDYLKNMTEFIESDKIVYKFSDSNNWSKDTFIKIYE
HHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]