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The map label for this gene is polA [H]

Identifier: 153951516

GI number: 153951516

Start: 1433150

End: 1435789

Strand: Direct

Name: polA [H]

Synonym: JJD26997_1620

Alternate gene names: 153951516

Gene position: 1433150-1435789 (Clockwise)

Preceding gene: 153952247

Following gene: 153952523

Centisome position: 77.67

GC content: 29.09

Gene sequence:

>2640_bases
ATGAAAACTTTAACTATTATCGATACTTTTGGATTTTTTTTCAGACTTTATTATGCCTTAAAAGGCTTTAAAAATTCACA
AGGACAAGCTAGTGGAATGGTTAGCGGTTTTGCTAATTTTATTTATGGTTTAAAAAACGAATATAAAAGCGATTATATTA
TTTTTGCACTGGATTCTAAGGGAAAAACTTTTCGTAGCGAAATTGATCCAAGCTATAAACAAAACCGCACTCCACCACCG
CCTGAACTTCTAGAACAAATTCCAATTTGTATAGAAATGATAGAAAAAATGGGTTTTATAAGCGTTTCTAGGGAAGGTTA
TGAAGCTGATGATATCATCGCTTCTTTTGTAAAAACTTGTGAAAATAAAGATATTTTTGTACGCATTATCACTCAAGATA
AAGATCTTTATCAACTCATAAAAGATGGGAAAACGAGCATTTACAGTCCTATTTCTAAAAACGACTATGATGAAGTAGCA
TGTTTAGAAAAATACGGGGTAAAACCTAATCAAATCAGAGATTTTTTAGCTCTTTGTGGAGATAACTCTGATAATATTCC
AGGCGTTAAAGGTATAGGTGCAAAAGGAGCTAAAACCTTACTTGATGAGTTTGGTAGCATAGAAGGAATTTATGAAAATT
TAACCCTAATACGCAACGAAAGAAGTCGTAATTTACTTTTAGAAGGTAAGGAAAATGCATTTTTAAGTAAAAAATTAGCT
TCTTTATACGAAAATTTAGAAGTGCAAGATCTTATAGAAAAAGCCACTTATCCTGATGAAGAACCTCTTTTAAAAATACT
TGAAATTTTAGAACATTACGAGCTTAATACTTTGCTTAAAAAACTGCGTCAAAATCCTGATAATAAAGATAAAAATTTAG
GTTTTAAAGCCACTTTAATACAAGATGAAAACAAACTTTTTGAAATTTTAAATACCCTTGATAAAGAAAGTATTATTGCT
TTTGATACCGAAACCACAGGACTTGATACAAAAGAAGCAAAAATTGTTGGTTTTAGTTTTTGCATGAGTGAAAATGAAGC
CTTTTATGTACCTTTAACTCATAATTATTTAGGTGTTAGCAAACAAATATCTTTAGAAAGTGCTAAAAAAGCTATAGAGC
TGATCTTTAATCATTTTGTTATAGGACACAATCTTAAATATGATTTTAAAATTATTCAAAACAATTTTGGATTAAATTTA
CCTCAAAAATACGCAGATACAATGATTTTAGCATGGCTTAAAAATCCTTCTTTAAGAGTAAATATGGATGATTTAGCCTT
AAGACTTTTTAATTACGAAACCTTACATTTTGAAAGTCTTGTTAAAAAAGGAGAAAATTTTGCAAGCGTAGAACTTGAAA
AAGCTTGCAAATACGCAGCGGAAGATGCTTATATCACTTTAAGATTTTATCTTTATTTTTTAAAAAATCTTGAAATTCCT
TTATTAGAACTTGCTAAAAACTGTGAATTTGATTTCATTAAAATCATTATGATGATGGAAGAAAATGGCATAAAACTTGA
TACAAATGCACTTGAAGCCCTTATGAAAAAATTTGAAAATGAAATCAAAATTTTAAGCGAAGAAATTTATACTCTTTGTG
AGGATAGATTTAACCTAAACTCACCTAAACAAGTAGGCGATATACTTTTTGAAAAACTCAAACTCCCAAGTGGAAAAAAG
GGAAAAACAGGATATTCTACCGATGAAAAAGTTTTACATGCACTTTTAGATAAACATCCTGTGATTACCAAAATTTTAGA
TTATAGAGAACTTGCCAAACTTTATTCCACTTATTGCGAACCTTTGTTAAAACTGGCTTTAAAAGATAAAAATTCACGCA
TTTATTCAAGTTTTTTACAGACTGGAACCGCTACAGGACGCCTTTCATCTAAAGATCCAAATTTACAAAACATCCCCGCA
CATGGACAATACGCAAAAGATTATAAATCTTGCTTTGTAGCTAAAGATGGATTTAGTTTTACCTCTCTTGATTATTCTCA
AATCGAACTTAGAATGCTCGCACATTTTAGCGAAGATGAAAAACTTTTAAATGCCTTTACCAATGATGAAGATATACACG
CAAGAACAGCTATAATGATTTTTGGAGAAAGCAATTATGAAACCAGAAGTGTTGCAAAAAGTATAAATTTTGGACTTATT
TATGGCATGGGCTATAAAACTTTAAGTCAAAATTTAAAAATAGAAGCTCATTTAGCCAAATCTTACATAGAAAAATATTT
TGAAAATTTTACTAGCATTAAAAAATACTTTGAAACAGTTAAAAATGAAGCTAAACAAAATGGTTTTATCACTACTTTAA
GCGGTCGCAAGCGTTATTTTGATTTTGAGAATGCAAAACCTATGCAAATTGCCATGTATGAAAGAGAAAGCATTAATTCA
ATCTTACAAGGCTCAGCAGCTGATATAATCAAACTTGCAATGCTAGAAATCAATAAAGAATTAAATGAAGACAAAAAGCT
TATTTTACAAATTCACGATGAATTAATTTTTGAAGTAAAAGATGATTTATGTGAAAATTTTGTAAAAAAAACTAGAGATA
TTATGGAAAATATAGTAAAATTGAAAGTAAAATTAAAAACAAGCTCAAGTATCGCCAAAAATTGGGGCGATTTAAAATAA

Upstream 100 bases:

>100_bases
GCGAATGGTTGCTTAAATTTATCCCTATCTTGCAACAACAATTTGAAAAAATTTCTAAAATTTTAATGTTATAATATCTC
ACTTTAAAGAAGGGAGTGGG

Downstream 100 bases:

>100_bases
TCAAGGAGATATTTAATGGATCTTTCAACCATACTGGGAATGGTGCTTGCAGTTACTAGTATTTCGGTAGGAGATATACT
AGAAGGAGGAAATCCTTTAC

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MKTLTIIDTFGFFFRLYYALKGFKNSQGQASGMVSGFANFIYGLKNEYKSDYIIFALDSKGKTFRSEIDPSYKQNRTPPP
PELLEQIPICIEMIEKMGFISVSREGYEADDIIASFVKTCENKDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEVA
CLEKYGVKPNQIRDFLALCGDNSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLIRNERSRNLLLEGKENAFLSKKLA
SLYENLEVQDLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLIQDENKLFEILNTLDKESIIA
FDTETTGLDTKEAKIVGFSFCMSENEAFYVPLTHNYLGVSKQISLESAKKAIELIFNHFVIGHNLKYDFKIIQNNFGLNL
PQKYADTMILAWLKNPSLRVNMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLEIP
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEALMKKFENEIKILSEEIYTLCEDRFNLNSPKQVGDILFEKLKLPSGKK
GKTGYSTDEKVLHALLDKHPVITKILDYRELAKLYSTYCEPLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPA
HGQYAKDYKSCFVAKDGFSFTSLDYSQIELRMLAHFSEDEKLLNAFTNDEDIHARTAIMIFGESNYETRSVAKSINFGLI
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYFDFENAKPMQIAMYERESINS
ILQGSAADIIKLAMLEINKELNEDKKLILQIHDELIFEVKDDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK

Sequences:

>Translated_879_residues
MKTLTIIDTFGFFFRLYYALKGFKNSQGQASGMVSGFANFIYGLKNEYKSDYIIFALDSKGKTFRSEIDPSYKQNRTPPP
PELLEQIPICIEMIEKMGFISVSREGYEADDIIASFVKTCENKDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEVA
CLEKYGVKPNQIRDFLALCGDNSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLIRNERSRNLLLEGKENAFLSKKLA
SLYENLEVQDLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLIQDENKLFEILNTLDKESIIA
FDTETTGLDTKEAKIVGFSFCMSENEAFYVPLTHNYLGVSKQISLESAKKAIELIFNHFVIGHNLKYDFKIIQNNFGLNL
PQKYADTMILAWLKNPSLRVNMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLEIP
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEALMKKFENEIKILSEEIYTLCEDRFNLNSPKQVGDILFEKLKLPSGKK
GKTGYSTDEKVLHALLDKHPVITKILDYRELAKLYSTYCEPLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPA
HGQYAKDYKSCFVAKDGFSFTSLDYSQIELRMLAHFSEDEKLLNAFTNDEDIHARTAIMIFGESNYETRSVAKSINFGLI
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYFDFENAKPMQIAMYERESINS
ILQGSAADIIKLAMLEINKELNEDKKLILQIHDELIFEVKDDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK
>Mature_879_residues
MKTLTIIDTFGFFFRLYYALKGFKNSQGQASGMVSGFANFIYGLKNEYKSDYIIFALDSKGKTFRSEIDPSYKQNRTPPP
PELLEQIPICIEMIEKMGFISVSREGYEADDIIASFVKTCENKDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEVA
CLEKYGVKPNQIRDFLALCGDNSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLIRNERSRNLLLEGKENAFLSKKLA
SLYENLEVQDLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLIQDENKLFEILNTLDKESIIA
FDTETTGLDTKEAKIVGFSFCMSENEAFYVPLTHNYLGVSKQISLESAKKAIELIFNHFVIGHNLKYDFKIIQNNFGLNL
PQKYADTMILAWLKNPSLRVNMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLEIP
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEALMKKFENEIKILSEEIYTLCEDRFNLNSPKQVGDILFEKLKLPSGKK
GKTGYSTDEKVLHALLDKHPVITKILDYRELAKLYSTYCEPLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPA
HGQYAKDYKSCFVAKDGFSFTSLDYSQIELRMLAHFSEDEKLLNAFTNDEDIHARTAIMIFGESNYETRSVAKSINFGLI
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYFDFENAKPMQIAMYERESINS
ILQGSAADIIKLAMLEINKELNEDKKLILQIHDELIFEVKDDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=439, Percent_Identity=31.6628701594533, Blast_Score=212, Evalue=1e-54,
Organism=Homo sapiens, GI139394648, Length=690, Percent_Identity=26.3768115942029, Blast_Score=200, Evalue=4e-51,
Organism=Escherichia coli, GI1790294, Length=920, Percent_Identity=35.9782608695652, Blast_Score=517, Evalue=1e-147,
Organism=Escherichia coli, GI226510970, Length=195, Percent_Identity=34.3589743589744, Blast_Score=104, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI86563272, Length=449, Percent_Identity=28.7305122494432, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17933644, Length=592, Percent_Identity=28.2094594594595, Blast_Score=203, Evalue=4e-52,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100855; Mature: 100855

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTIIDTFGFFFRLYYALKGFKNSQGQASGMVSGFANFIYGLKNEYKSDYIIFALDSK
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
GKTFRSEIDPSYKQNRTPPPPELLEQIPICIEMIEKMGFISVSREGYEADDIIASFVKTC
CCHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHC
ENKDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEVACLEKYGVKPNQIRDFLALCG
CCCCEEEEEEECCHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
DNSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLIRNERSRNLLLEGKENAFLSKKLA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHH
SLYENLEVQDLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLI
HHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
QDENKLFEILNTLDKESIIAFDTETTGLDTKEAKIVGFSFCMSENEAFYVPLTHNYLGVS
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCC
KQISLESAKKAIELIFNHFVIGHNLKYDFKIIQNNFGLNLPQKYADTMILAWLKNPSLRV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEECCCCEEE
NMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLEIP
EHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCEEHHHHHHHHHHCCCCC
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEALMKKFENEIKILSEEIYTLCEDRFNLN
HHHHHCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPKQVGDILFEKLKLPSGKKGKTGYSTDEKVLHALLDKHPVITKILDYRELAKLYSTYCE
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
PLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPAHGQYAKDYKSCFVAKDGFSF
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCE
TSLDYSQIELRMLAHFSEDEKLLNAFTNDEDIHARTAIMIFGESNYETRSVAKSINFGLI
ECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCEEE
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYF
EECCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEC
DFENAKPMQIAMYERESINSILQGSAADIIKLAMLEINKELNEDKKLILQIHDELIFEVK
CCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
DDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK
HHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCC
>Mature Secondary Structure
MKTLTIIDTFGFFFRLYYALKGFKNSQGQASGMVSGFANFIYGLKNEYKSDYIIFALDSK
CCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
GKTFRSEIDPSYKQNRTPPPPELLEQIPICIEMIEKMGFISVSREGYEADDIIASFVKTC
CCHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHC
ENKDIFVRIITQDKDLYQLIKDGKTSIYSPISKNDYDEVACLEKYGVKPNQIRDFLALCG
CCCCEEEEEEECCHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHC
DNSDNIPGVKGIGAKGAKTLLDEFGSIEGIYENLTLIRNERSRNLLLEGKENAFLSKKLA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHH
SLYENLEVQDLIEKATYPDEEPLLKILEILEHYELNTLLKKLRQNPDNKDKNLGFKATLI
HHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEE
QDENKLFEILNTLDKESIIAFDTETTGLDTKEAKIVGFSFCMSENEAFYVPLTHNYLGVS
CCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCC
KQISLESAKKAIELIFNHFVIGHNLKYDFKIIQNNFGLNLPQKYADTMILAWLKNPSLRV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHEECCCCEEE
NMDDLALRLFNYETLHFESLVKKGENFASVELEKACKYAAEDAYITLRFYLYFLKNLEIP
EHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCEEHHHHHHHHHHCCCCC
LLELAKNCEFDFIKIIMMMEENGIKLDTNALEALMKKFENEIKILSEEIYTLCEDRFNLN
HHHHHCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPKQVGDILFEKLKLPSGKKGKTGYSTDEKVLHALLDKHPVITKILDYRELAKLYSTYCE
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
PLLKLALKDKNSRIYSSFLQTGTATGRLSSKDPNLQNIPAHGQYAKDYKSCFVAKDGFSF
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEECCCCCE
TSLDYSQIELRMLAHFSEDEKLLNAFTNDEDIHARTAIMIFGESNYETRSVAKSINFGLI
ECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCHHHHHHHHCCCEEE
YGMGYKTLSQNLKIEAHLAKSYIEKYFENFTSIKKYFETVKNEAKQNGFITTLSGRKRYF
EECCHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEC
DFENAKPMQIAMYERESINSILQGSAADIIKLAMLEINKELNEDKKLILQIHDELIFEVK
CCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
DDLCENFVKKTRDIMENIVKLKVKLKTSSSIAKNWGDLK
HHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]