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The map label for this gene is miaB [H]

Identifier: 153951503

GI number: 153951503

Start: 1314879

End: 1316180

Strand: Direct

Name: miaB [H]

Synonym: JJD26997_1481

Alternate gene names: 153951503

Gene position: 1314879-1316180 (Clockwise)

Preceding gene: 153951837

Following gene: 153952130

Centisome position: 71.26

GC content: 32.33

Gene sequence:

>1302_bases
TTGAGTGCTAAAAAACTTTTTATTCAAACTTTAGGTTGTGCGATGAATGTCAGAGATTCTGAGCATATGATTGCGGAATT
AACACAAAAAGAAAATTACGCACTAACAGAAGATATCAAAGAAGCAGATCTTATTCTTATCAATACCTGTTCAGTGCGTG
AAAAACCTGTGCATAAACTTTTCTCAGAAGTGGGTGGCTTTGAAAAAGTTAAAAAAGAAGGTGCTAAAATAGGAGTTTGT
GGATGTACTGCATCACATTTGGGAAATGAAATTTTTAAACGTGCTCCTTATGTGGATTTTGTTCTAGGGGCAAGAAATAT
TTCCAAAATCACCCAAGCTATAAAAACTCCAAAATTTATGGGCATAGATATAGACTATGATGAAAGTGAATTTGCTTTTG
CAGATTTTAGAAATAGTATTTATAAATCCTATATTAATATCTCCATAGGTTGCGACAAACACTGCACTTATTGTATAGTG
CCACACACAAGAGGAGATGAAATTTCCATACCTTTTAACATCATCCATAAAGAAGCTCAAAAAGCCGTTGAAAAAGGTGC
TAAAGAGATTTTCTTGCTAGGACAAAATGTCAATAACTACGGCAAAAGATTTAGAAATGAACATAAAAAAATGGACTTTT
CAGATCTTTTAGAAGAACTTAGCACCATAGAAGATTTAGAACGCATTCGCTTTACAAGTCCACATCCTTTACACATGGAT
GATAAATTTTTGGAAGTTTTTGCCAATAATCCAAAAGTTTGCAAATCTATGCACATGCCTTTACAAAGCGGTTCAAGTGA
AATTTTAAAAGCTATGAAGCGTGGCTATACTAAAAAATGGTATTTAAATAGAGCTTTAAAACTTAGAGAACTTTGTCCAA
ATGTCAGCATTTCTACAGATATTATTGTAGCTTTTCCAGGAGAAAGTGAAAAAGATTTTGAAGAAACCATGGATGTTTTA
GAAAAAGTGCGTTTTGAACAAATTTTTTCTTTTAAATACTCCAAACGCCCTTTAACAAAAGCCGCAACCATGTCCAATCA
AATCGATGAAGAAACAGCTTCAAGAAGACTTAGCACCTTGCAAAATCGTCATAGTGAAATTTTAGATGAAATTGTAAAAA
AACAAGAAAATAAAACTTTTAAAGTTTTATTTGAAGAATTAAGAGTGGGCAATAGCATTGCTGGAAGAACGGATAATAAT
TTTTTAGTTCAAGTTGAAGGTAGCGAAGAACTTTTAGGGCAATTTAAAGAAGTAAAAATCACTAATGCAAAGCGTATGGT
TTTATATGGGGAAATCATTTAA

Upstream 100 bases:

>100_bases
CCCTTGTAAATCATTTTGAAAAAAAAGGAGTAAGCATTTATCATAGAACCGTAAAATACGGACTTGATGACAATGATTTT
ATGTATGAGGTGCATATTCT

Downstream 100 bases:

>100_bases
AATTTATTGTATAACTTATATAGTTTTTATTTTACAATGGCTGATTTTTCTAAGTTGTAAAAAAAAATACAAAGGGGGAA
AAATCGACCATAAACCCTAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKLFSEVGGFEKVKKEGAKIGVC
GCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFMGIDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIV
PHTRGDEISIPFNIIHKEAQKAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKKWYLNRALKLRELCPNVSISTDIIVAFPGESEKDFEETMDVL
EKVRFEQIFSFKYSKRPLTKAATMSNQIDEETASRRLSTLQNRHSEILDEIVKKQENKTFKVLFEELRVGNSIAGRTDNN
FLVQVEGSEELLGQFKEVKITNAKRMVLYGEII

Sequences:

>Translated_433_residues
MSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKLFSEVGGFEKVKKEGAKIGVC
GCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFMGIDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIV
PHTRGDEISIPFNIIHKEAQKAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKKWYLNRALKLRELCPNVSISTDIIVAFPGESEKDFEETMDVL
EKVRFEQIFSFKYSKRPLTKAATMSNQIDEETASRRLSTLQNRHSEILDEIVKKQENKTFKVLFEELRVGNSIAGRTDNN
FLVQVEGSEELLGQFKEVKITNAKRMVLYGEII
>Mature_432_residues
SAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKLFSEVGGFEKVKKEGAKIGVCG
CTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFMGIDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVP
HTRGDEISIPFNIIHKEAQKAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMDD
KFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKKWYLNRALKLRELCPNVSISTDIIVAFPGESEKDFEETMDVLE
KVRFEQIFSFKYSKRPLTKAATMSNQIDEETASRRLSTLQNRHSEILDEIVKKQENKTFKVLFEELRVGNSIAGRTDNNF
LVQVEGSEELLGQFKEVKITNAKRMVLYGEII

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=32.2851153039832, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=32.2851153039832, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI93277076, Length=374, Percent_Identity=27.0053475935829, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=36.6292134831461, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1787057, Length=442, Percent_Identity=28.0542986425339, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=29.5258620689655, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI71996771, Length=362, Percent_Identity=27.3480662983425, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=27.6729559748428, Blast_Score=207, Evalue=2e-53,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=28.9473684210526, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49559; Mature: 49427

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50926 TRAM ; PS00133 CARBOXYPEPT_ZN_2 ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKL
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCHHHHHHH
FSEVGGFEKVKKEGAKIGVCGCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFM
HHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCCCCEE
GIDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVPHTRGDEISIPFNIIHKEAQ
EEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCEEECHHHHHHHHHH
KAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
HHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKKWYLNRALKLRELCPNVSISTD
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
IIVAFPGESEKDFEETMDVLEKVRFEQIFSFKYSKRPLTKAATMSNQIDEETASRRLSTL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QNRHSEILDEIVKKQENKTFKVLFEELRVGNSIAGRTDNNFLVQVEGSEELLGQFKEVKI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHEEE
TNAKRMVLYGEII
CCCCEEEEEECCC
>Mature Secondary Structure 
SAKKLFIQTLGCAMNVRDSEHMIAELTQKENYALTEDIKEADLILINTCSVREKPVHKL
CCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCCCEEEEEECCCCHHHHHHH
FSEVGGFEKVKKEGAKIGVCGCTASHLGNEIFKRAPYVDFVLGARNISKITQAIKTPKFM
HHHHCCHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHCCCCEE
GIDIDYDESEFAFADFRNSIYKSYINISIGCDKHCTYCIVPHTRGDEISIPFNIIHKEAQ
EEEECCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCCEEECHHHHHHHHHH
KAVEKGAKEIFLLGQNVNNYGKRFRNEHKKMDFSDLLEELSTIEDLERIRFTSPHPLHMD
HHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DKFLEVFANNPKVCKSMHMPLQSGSSEILKAMKRGYTKKWYLNRALKLRELCPNVSISTD
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
IIVAFPGESEKDFEETMDVLEKVRFEQIFSFKYSKRPLTKAATMSNQIDEETASRRLSTL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QNRHSEILDEIVKKQENKTFKVLFEELRVGNSIAGRTDNNFLVQVEGSEELLGQFKEVKI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHEEE
TNAKRMVLYGEII
CCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA