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The map label for this gene is yafV [C]

Identifier: 153951401

GI number: 153951401

Start: 607881

End: 608720

Strand: Direct

Name: yafV [C]

Synonym: JJD26997_0671

Alternate gene names: 153951401

Gene position: 607881-608720 (Clockwise)

Preceding gene: 153952650

Following gene: 153952240

Centisome position: 32.95

GC content: 39.76

Gene sequence:

>840_bases
ATGCGAAGAAAATTAAAAGCGGCCCTAATTCAGTTTGCTCCCCTATCTTATGAAAGAGAACAGAATTTAAACAAGTCTTT
AATCTTTACCCAAACAGCTCTAGAGCAAGGAGCCAAGCTTGTGGTGTTACCTGAGCTTTTTGATAGTGGGTATTGCGTGG
AAGATAAGGATTTTTCTTACGCTCTTTCTTTGGAAAAAGAACTTCCCTCTAGCTTAAAAGAACTTGCGAAAAAATATGAA
GCGTACATTATTGCTTGTAGTATAGAAAAAGAGGATGATACACTCTATGATACAGCTTTCATCGTGGGTAAAGAGGGGCT
TGTGGGCAAATACCGCAAGATTTATCTTTTTCGCGATGAGAAAAAGCGTTTTGCAAGGGGGCAGGATTATCCCGTGTTTG
AGCTAGTTTTTAAGGATTTTAGTCTTAAACTTGGCTTGCAAATTTGCTATGAAATAGGCTTTGGCGAGGGGGCTAGGTTT
TTAGCCTTGCAAGGAGCAGAGCTCATCTGCTATCCTTCCGCCTTTGGCAAGGCTAGGGCTTATGTGTGGGATTTAGCTTC
TAGGGCTAGAGCTTTAGAAAGCGGAGCCTTTGTCCTAGCGGCAAATCGCTGTGGGAGTGAAATTTCTAAGCTAAGTGGTG
AGAACTTAGAATTTGCAGGGCGTTCTAAAATCATTAATCCTAAGGGCGAAATTCTTAGCGAACTTAGCGAAAAAGAAGGC
ATAAGCTACGCTGTGCTTGATATAACCCAGGTAAAGGCACAAAGAGAATATCTTACTTATCTTAAGGATTTAGATTTAGA
TTTAAGTCAAAAAATGTATGCTAAATTCAATTTATCTTAA

Upstream 100 bases:

>100_bases
TTATGAAGCTTGTAGAGAAAGATTTTGAATTAAGATTTTAAAAATAAGTTTAAATTTTAACATAAATTTAAATACAGATG
AAAAATATGAAAGTAAATCC

Downstream 100 bases:

>100_bases
CAAAAAGGAGTAAAAAATGGCAAAAGAAATTTTAGTTGCTTATGGCGTGGATATTGATGCAGTAGCGGGTTGGCTAGGAA
GTTATGGGGGAGAGGATTCG

Product: carbon-nitrogen hydrolase

Products: NA

Alternate protein names: ORF 5 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRRKLKAALIQFAPLSYEREQNLNKSLIFTQTALEQGAKLVVLPELFDSGYCVEDKDFSYALSLEKELPSSLKELAKKYE
AYIIACSIEKEDDTLYDTAFIVGKEGLVGKYRKIYLFRDEKKRFARGQDYPVFELVFKDFSLKLGLQICYEIGFGEGARF
LALQGAELICYPSAFGKARAYVWDLASRARALESGAFVLAANRCGSEISKLSGENLEFAGRSKIINPKGEILSELSEKEG
ISYAVLDITQVKAQREYLTYLKDLDLDLSQKMYAKFNLS

Sequences:

>Translated_279_residues
MRRKLKAALIQFAPLSYEREQNLNKSLIFTQTALEQGAKLVVLPELFDSGYCVEDKDFSYALSLEKELPSSLKELAKKYE
AYIIACSIEKEDDTLYDTAFIVGKEGLVGKYRKIYLFRDEKKRFARGQDYPVFELVFKDFSLKLGLQICYEIGFGEGARF
LALQGAELICYPSAFGKARAYVWDLASRARALESGAFVLAANRCGSEISKLSGENLEFAGRSKIINPKGEILSELSEKEG
ISYAVLDITQVKAQREYLTYLKDLDLDLSQKMYAKFNLS
>Mature_279_residues
MRRKLKAALIQFAPLSYEREQNLNKSLIFTQTALEQGAKLVVLPELFDSGYCVEDKDFSYALSLEKELPSSLKELAKKYE
AYIIACSIEKEDDTLYDTAFIVGKEGLVGKYRKIYLFRDEKKRFARGQDYPVFELVFKDFSLKLGLQICYEIGFGEGARF
LALQGAELICYPSAFGKARAYVWDLASRARALESGAFVLAANRCGSEISKLSGENLEFAGRSKIINPKGEILSELSEKEG
ISYAVLDITQVKAQREYLTYLKDLDLDLSQKMYAKFNLS

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=280, Percent_Identity=31.7857142857143, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI297632348, Length=273, Percent_Identity=27.1062271062271, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI5031947, Length=273, Percent_Identity=27.1062271062271, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI297632350, Length=273, Percent_Identity=27.1062271062271, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1786412, Length=232, Percent_Identity=26.7241379310345, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=258, Percent_Identity=25.5813953488372, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17533173, Length=223, Percent_Identity=27.3542600896861, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=292, Percent_Identity=28.4246575342466, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6322335, Length=317, Percent_Identity=26.813880126183, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355835, Length=283, Percent_Identity=27.9151943462898, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17933642, Length=268, Percent_Identity=29.4776119402985, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21358471, Length=292, Percent_Identity=27.0547945205479, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 31567; Mature: 31567

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKLKAALIQFAPLSYEREQNLNKSLIFTQTALEQGAKLVVLPELFDSGYCVEDKDFSY
CCHHHHHHHHHHCCCCCHHHCCCCCEEEEEHHHHHHCCEEEEEHHHHCCCCEECCCCCEE
ALSLEKELPSSLKELAKKYEAYIIACSIEKEDDTLYDTAFIVGKEGLVGKYRKIYLFRDE
EEEECHHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEEECC
KKRFARGQDYPVFELVFKDFSLKLGLQICYEIGFGEGARFLALQGAELICYPSAFGKARA
HHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCEEEEECCCCCCHHH
YVWDLASRARALESGAFVLAANRCGSEISKLSGENLEFAGRSKIINPKGEILSELSEKEG
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCC
ISYAVLDITQVKAQREYLTYLKDLDLDLSQKMYAKFNLS
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCC
>Mature Secondary Structure
MRRKLKAALIQFAPLSYEREQNLNKSLIFTQTALEQGAKLVVLPELFDSGYCVEDKDFSY
CCHHHHHHHHHHCCCCCHHHCCCCCEEEEEHHHHHHCCEEEEEHHHHCCCCEECCCCCEE
ALSLEKELPSSLKELAKKYEAYIIACSIEKEDDTLYDTAFIVGKEGLVGKYRKIYLFRDE
EEEECHHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEEECCCCCCCEEEEEEEECC
KKRFARGQDYPVFELVFKDFSLKLGLQICYEIGFGEGARFLALQGAELICYPSAFGKARA
HHHHHCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCEEEEECCCCCCHHH
YVWDLASRARALESGAFVLAANRCGSEISKLSGENLEFAGRSKIINPKGEILSELSEKEG
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCC
ISYAVLDITQVKAQREYLTYLKDLDLDLSQKMYAKFNLS
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8359673 [H]