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The map label for this gene is purH [H]

Identifier: 153951239

GI number: 153951239

Start: 747245

End: 748777

Strand: Direct

Name: purH [H]

Synonym: JJD26997_0828

Alternate gene names: 153951239

Gene position: 747245-748777 (Clockwise)

Preceding gene: 153951540

Following gene: 153951325

Centisome position: 40.5

GC content: 33.86

Gene sequence:

>1533_bases
ATGAGAGCATTGCTTAGTGTGAGTGATAAAGAAGGCATAGTGGAATTTGGCAAAGAGCTTGAAAATTTAGGCTTTGAGAT
TCTTTCAACAGGTGGGACTTTTAAGCTTTTAAAAGAAAATGGAGTAAAAGTTATAGAAGTGAGTGATTTTACAAAAAGTC
CTGAGCTTTTTGAAGGGCGTGTGAAGACTTTGCATCCTAAAATTCATGGTGGAATTTTACACAAAAGAAACGATGAAAAT
CATATCAAACAAGCAAAAGAAAATGAAATTTTAGGCATTGATCTAGTTTGTGTAAATTTGTATCCTTTTAAAAAAACCAC
TATTATGAGTGATGATTTTGATGAGATTATAGAAAATATTGACATAGGCGGGCCTGCGATGATAAGAAGTGCGGCAAAAA
ACTATAAAGATGTTATGGTGCTTTGCGATCGGCTTGATTATGAAAAAGTTATTGAGACTTTAAAAAAGAATCAAAATGAT
GAAAATTTTCGCCTGAGTTTAATGATAAAGGCTTATGAGTATACGGCAAATTACGATGCTTATATTGCAAATTATATGAA
TGAACGCTTTAACGGAGGTTTTGGAGCGAGTAAATTTATAGTAGGACAAAAAGTATTTGATACAAAATATGGAGAAAATC
CACATCAAAAAGGAGCTTTATACGAGTTTGATGCCTTTTTTAGTGCAAATTTCAAAGCCCTTAAAGGCGAAGCAAGTTTT
AACAATCTAACCGATATTAACGCAGCTTTAAACTTAGCTAGTAGTTTTGATAAAGCTCCAGCTATTGCTATAGTAAAACA
TGGAAATTCTTGTGGTTTTGCTATAAAAGAAAATTTAGTACAAAGCTATATACATGCTTTAAAATCCGATAGCGTTAGTG
CTTATGGAGGAGTGGTTGCAATCAATGGAACTCTTGATGAGGCTCTAGCAAATAAAATCAATGAAATTTATGTAGAAGTA
ATCATCGCAGCCAATGTAGATGAAAAAGCTTTAGCTGTGTTTGAAGGTAAAAAACGCATTAAAATTTTCACTCAAGAAAG
TCCGTTTTTAATTCGTAGTTTTGATAAATATGATTTTAAACATATTGATGGGGGTTTTGTGTATCAAAACAGCGATGAAG
CGGATGAAGATGAACTTAAAAATGCAAAGCTTATGAGTCAAAGAGAGGCAAGCAAGGAAGAATTAAAAGATCTTGAAATC
GCTATGAAAATAGCAGCATTTACCAAATCAAACAATGTTGTTTATGTAAAAAATGGCGCTATGGTGGCTATAGGTATGGG
TATGACAAGCAGAATCGATGCAGCAAAGGCAGCTACTTTTAAAGCCAAAGAAATGGGGCTTGATTTACAAGGTTGCGTTT
TAGCAAGTGAAGCCTTTTTTCCATTTAGAGATAGCATAGATGAAGCAAGTAAAGTAGGGGTTAAAGCTATAGTAGAACCA
GGTGGAAGTATAAGAGATGATGAAGTAGTAAAGGCGGCTGATGAGTATGGTATGGCGCTTTATTTCACAGGAGTAAGACA
TTTCTTACATTAA

Upstream 100 bases:

>100_bases
CAAACAATGTCAGCGAAGAAGAGCTTAAAATCGGCGTAGAGAAATTTCAAAAAATCAACGAAGCTTATGAAAAAGTTAAA
AAACATTTAGAAAGGTAAAA

Downstream 100 bases:

>100_bases
GTTTAATGGAATGTTATGTTTTTTACATAATTTAATAAAAATTAAATAAATTATATGTAAAATATTTCCGAAAAATTTTA
TTTCTAGGGAACTGTGATGT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGVKVIEVSDFTKSPELFEGRVKTLHPKIHGGILHKRNDEN
HIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDRLDYEKVIETLKKNQND
ENFRLSLMIKAYEYTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
NNLTDINAALNLASSFDKAPAIAIVKHGNSCGFAIKENLVQSYIHALKSDSVSAYGGVVAINGTLDEALANKINEIYVEV
IIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEADEDELKNAKLMSQREASKEELKDLEI
AMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAATFKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
GGSIRDDEVVKAADEYGMALYFTGVRHFLH

Sequences:

>Translated_510_residues
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGVKVIEVSDFTKSPELFEGRVKTLHPKIHGGILHKRNDEN
HIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDRLDYEKVIETLKKNQND
ENFRLSLMIKAYEYTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
NNLTDINAALNLASSFDKAPAIAIVKHGNSCGFAIKENLVQSYIHALKSDSVSAYGGVVAINGTLDEALANKINEIYVEV
IIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEADEDELKNAKLMSQREASKEELKDLEI
AMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAATFKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
GGSIRDDEVVKAADEYGMALYFTGVRHFLH
>Mature_510_residues
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGVKVIEVSDFTKSPELFEGRVKTLHPKIHGGILHKRNDEN
HIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDRLDYEKVIETLKKNQND
ENFRLSLMIKAYEYTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
NNLTDINAALNLASSFDKAPAIAIVKHGNSCGFAIKENLVQSYIHALKSDSVSAYGGVVAINGTLDEALANKINEIYVEV
IIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEADEDELKNAKLMSQREASKEELKDLEI
AMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAATFKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
GGSIRDDEVVKAADEYGMALYFTGVRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=32.1649484536082, Blast_Score=208, Evalue=8e-54,
Organism=Escherichia coli, GI1790439, Length=534, Percent_Identity=44.0074906367041, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71985564, Length=501, Percent_Identity=31.3373253493014, Blast_Score=220, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI71985574, Length=333, Percent_Identity=25.5255255255255, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=50, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323768, Length=474, Percent_Identity=31.4345991561181, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6323056, Length=485, Percent_Identity=31.5463917525773, Blast_Score=209, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24649832, Length=473, Percent_Identity=33.1923890063425, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56599; Mature: 56599

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGVKVIEVSDFTKSPELFEGR
CCCEECCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEECCCCCCCHHHCCH
VKTLHPKIHGGILHKRNDENHIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENI
HHHCCCHHHCCCEECCCCHHHHHHHHCCCEEEEEEEEEEEECCCCEEECCCCHHHHHHHC
DIGGPAMIRSAAKNYKDVMVLCDRLDYEKVIETLKKNQNDENFRLSLMIKAYEYTANYDA
CCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCHH
YIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
HHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEHHHHCCCCEEEECCCCC
NNLTDINAALNLASSFDKAPAIAIVKHGNSCGFAIKENLVQSYIHALKSDSVSAYGGVVA
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
INGTLDEALANKINEIYVEVIIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFK
ECCCHHHHHHHHHHHEEEEEEEEECCCHHHHHEECCCCEEEEEECCCCCEEEECCCCCCE
HIDGGFVYQNSDEADEDELKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGA
ECCCCEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCEEEEECCC
MVAIGMGMTSRIDAAKAATFKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
EEEEECCCCCCCCHHHHHHEEHHHHCCCHHHEEEECCCCCCCHHCHHHHHHCCEEEEECC
GGSIRDDEVVKAADEYGMALYFTGVRHFLH
CCCCCCHHHHHHHHHCCEEEEEEHHHHHCC
>Mature Secondary Structure
MRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGVKVIEVSDFTKSPELFEGR
CCCEECCCCCCHHHHHHHHHHHCCEEEEECCCEEEEEECCCCEEEEECCCCCCCHHHCCH
VKTLHPKIHGGILHKRNDENHIKQAKENEILGIDLVCVNLYPFKKTTIMSDDFDEIIENI
HHHCCCHHHCCCEECCCCHHHHHHHHCCCEEEEEEEEEEEECCCCEEECCCCHHHHHHHC
DIGGPAMIRSAAKNYKDVMVLCDRLDYEKVIETLKKNQNDENFRLSLMIKAYEYTANYDA
CCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCCHH
YIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEFDAFFSANFKALKGEASF
HHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCCEEEEHHHHCCCCEEEECCCCC
NNLTDINAALNLASSFDKAPAIAIVKHGNSCGFAIKENLVQSYIHALKSDSVSAYGGVVA
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
INGTLDEALANKINEIYVEVIIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFK
ECCCHHHHHHHHHHHEEEEEEEEECCCHHHHHEECCCCEEEEEECCCCCEEEECCCCCCE
HIDGGFVYQNSDEADEDELKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGA
ECCCCEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCEEEEECCC
MVAIGMGMTSRIDAAKAATFKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEP
EEEEECCCCCCCCHHHHHHEEHHHHCCCHHHEEEECCCCCCCHHCHHHHHHCCEEEEECC
GGSIRDDEVVKAADEYGMALYFTGVRHFLH
CCCCCCHHHHHHHHHCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA