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The map label for this gene is purF [H]

Identifier: 153951078

GI number: 153951078

Start: 655471

End: 656604

Strand: Direct

Name: purF [H]

Synonym: JJD26997_0725

Alternate gene names: 153951078

Gene position: 655471-656604 (Clockwise)

Preceding gene: 153951812

Following gene: 153952102

Centisome position: 35.52

GC content: 27.34

Gene sequence:

>1134_bases
ATGTTTGCCATCTTCTTTCAAAAATTTGGAGTGGCAAAAATGCTCTTAAAACCTTTGCCACAATTTTTAGAATGCATTAT
CTTAGATAAGAATTTATTACATTCTAAATTAAAGCTTTATCTTGAAAGATATTTGACTAAAAATTACGCAATTGTAGCTG
AAGAGAGTTTTGCAGAGTATTTTAAAATTTTAAAATTTAACAATATTATTTTAAAACAAACAGAATTTAATAAATATCAA
AAAATATTACAAAATCAAAAAACTCACATTTTTGTTTTAAACAAGGGTGCGAAATTTAAAATCAAAAAAGACTCAAACAT
TACTCACATCACTATACCAAAAATGTCCGCTTTTGATAACACAAATAATAAGTTATATAAAGGAAAAATATTAGCTGATA
ATCTTGCTGAAAATATCGTATTATCTATAAAAAATAAAGAAAAAATGAAAAATCCACAAGAAAATAGCCTTATCCCTCTT
AAACAAAAATACTGCTCTTTAAGACTTCTTTATAAAAATGATGTTAATGATATTATCTTGGGACAAAATGTAGGAGAATT
TCGCATTAAACTAGGGAAAATTTTAGCTTCTTACATCGATAAAAGACTTATTAAAAATTTGGACTATATCGTTGGAGTGC
CATCATCTGGAATTTTTTATGCCGTCGGTCTTTCACAAGCTTTAAATATCCCATTTTTACCCGCACTAAAAAAACAAGAA
GTTAGTGAAAGGTCTTTTGAAATCGTCAATACAGATATAAGAAAAAATTATTTATACTCTAATATCCAAATTAACTCTCA
CCTCATCAAAGATAAAAAAATCCTACTTGTCGATGAGGCTATTTTTACAGGAGCTACACTTAAAATCGTTTGTGATATTT
TAAGAGATAATAAGGTAAAAGAAAAAATTCACTTAGCTATACCTTCTCCTAAATGCTACAAGCAATGTGATTATCTTATG
CTGCCAAAAAGGACCTTGTTGCTTGAAAAAATAAAGGAAAATTTGATGGCGCAATATTTAAATGCGGACTCTTTGACATT
TTTGCCACAAAAAGCCTATAAAAATGCCCTTATACAAATTAATAAAAATCTATGCTTAGAATGTTTTGAAAACAAAGGAG
AAGTAAATGTTTAG

Upstream 100 bases:

>100_bases
TAGAAGTGTGTGTCTTTATTTACAATCTATCATTTTGTGTGATTATTTGCTTACAAATTTACAAGGCATTAAAACACACA
TACGGGGGGGGGGTAAATAG

Downstream 100 bases:

>100_bases
TGCCATTATTCCTATTCGAAAAAGCAAATATCAATTTGAAATTTTACCTTGCAATGCAAAAAATTTACTAGAAAGAAAAG
TGTTTTATCTTAAAAAAATT

Product: putative glutamine amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MFAIFFQKFGVAKMLLKPLPQFLECIILDKNLLHSKLKLYLERYLTKNYAIVAEESFAEYFKILKFNNIILKQTEFNKYQ
KILQNQKTHIFVLNKGAKFKIKKDSNITHITIPKMSAFDNTNNKLYKGKILADNLAENIVLSIKNKEKMKNPQENSLIPL
KQKYCSLRLLYKNDVNDIILGQNVGEFRIKLGKILASYIDKRLIKNLDYIVGVPSSGIFYAVGLSQALNIPFLPALKKQE
VSERSFEIVNTDIRKNYLYSNIQINSHLIKDKKILLVDEAIFTGATLKIVCDILRDNKVKEKIHLAIPSPKCYKQCDYLM
LPKRTLLLEKIKENLMAQYLNADSLTFLPQKAYKNALIQINKNLCLECFENKGEVNV

Sequences:

>Translated_377_residues
MFAIFFQKFGVAKMLLKPLPQFLECIILDKNLLHSKLKLYLERYLTKNYAIVAEESFAEYFKILKFNNIILKQTEFNKYQ
KILQNQKTHIFVLNKGAKFKIKKDSNITHITIPKMSAFDNTNNKLYKGKILADNLAENIVLSIKNKEKMKNPQENSLIPL
KQKYCSLRLLYKNDVNDIILGQNVGEFRIKLGKILASYIDKRLIKNLDYIVGVPSSGIFYAVGLSQALNIPFLPALKKQE
VSERSFEIVNTDIRKNYLYSNIQINSHLIKDKKILLVDEAIFTGATLKIVCDILRDNKVKEKIHLAIPSPKCYKQCDYLM
LPKRTLLLEKIKENLMAQYLNADSLTFLPQKAYKNALIQINKNLCLECFENKGEVNV
>Mature_377_residues
MFAIFFQKFGVAKMLLKPLPQFLECIILDKNLLHSKLKLYLERYLTKNYAIVAEESFAEYFKILKFNNIILKQTEFNKYQ
KILQNQKTHIFVLNKGAKFKIKKDSNITHITIPKMSAFDNTNNKLYKGKILADNLAENIVLSIKNKEKMKNPQENSLIPL
KQKYCSLRLLYKNDVNDIILGQNVGEFRIKLGKILASYIDKRLIKNLDYIVGVPSSGIFYAVGLSQALNIPFLPALKKQE
VSERSFEIVNTDIRKNYLYSNIQINSHLIKDKKILLVDEAIFTGATLKIVCDILRDNKVKEKIHLAIPSPKCYKQCDYLM
LPKRTLLLEKIKENLMAQYLNADSLTFLPQKAYKNALIQINKNLCLECFENKGEVNV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17554892, Length=221, Percent_Identity=27.6018099547511, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24659604, Length=207, Percent_Identity=28.0193236714976, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24659598, Length=205, Percent_Identity=27.3170731707317, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 43626; Mature: 43626

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAIFFQKFGVAKMLLKPLPQFLECIILDKNLLHSKLKLYLERYLTKNYAIVAEESFAEY
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
FKILKFNNIILKQTEFNKYQKILQNQKTHIFVLNKGAKFKIKKDSNITHITIPKMSAFDN
HHHHHHCCEEEECCHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCC
TNNKLYKGKILADNLAENIVLSIKNKEKMKNPQENSLIPLKQKYCSLRLLYKNDVNDIIL
CCCEEEEEEEEHHHHHCCEEEEECCHHHCCCCCCCCCCCHHHHHCEEEEEEECCCCCEEE
GQNVGEFRIKLGKILASYIDKRLIKNLDYIVGVPSSGIFYAVGLSQALNIPFLPALKKQE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEHHHHCCCCCCCCHHHHH
VSERSFEIVNTDIRKNYLYSNIQINSHLIKDKKILLVDEAIFTGATLKIVCDILRDNKVK
HHHHHHHHHHHHHHHHHEEEEEEECCEEECCCEEEEEECHHHCCCHHHHHHHHHHCCCCC
EKIHLAIPSPKCYKQCDYLMLPKRTLLLEKIKENLMAQYLNADSLTFLPQKAYKNALIQI
CEEEEECCCCHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHCCCEEEE
NKNLCLECFENKGEVNV
CCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFAIFFQKFGVAKMLLKPLPQFLECIILDKNLLHSKLKLYLERYLTKNYAIVAEESFAEY
CEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
FKILKFNNIILKQTEFNKYQKILQNQKTHIFVLNKGAKFKIKKDSNITHITIPKMSAFDN
HHHHHHCCEEEECCHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCC
TNNKLYKGKILADNLAENIVLSIKNKEKMKNPQENSLIPLKQKYCSLRLLYKNDVNDIIL
CCCEEEEEEEEHHHHHCCEEEEECCHHHCCCCCCCCCCCHHHHHCEEEEEEECCCCCEEE
GQNVGEFRIKLGKILASYIDKRLIKNLDYIVGVPSSGIFYAVGLSQALNIPFLPALKKQE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEHHHHCCCCCCCCHHHHH
VSERSFEIVNTDIRKNYLYSNIQINSHLIKDKKILLVDEAIFTGATLKIVCDILRDNKVK
HHHHHHHHHHHHHHHHHEEEEEEECCEEECCCEEEEEECHHHCCCHHHHHHHHHHCCCCC
EKIHLAIPSPKCYKQCDYLMLPKRTLLLEKIKENLMAQYLNADSLTFLPQKAYKNALIQI
CEEEEECCCCHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHCCCEEEE
NKNLCLECFENKGEVNV
CCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]