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The map label for this gene is nfi

Identifier: 153950857

GI number: 153950857

Start: 4334421

End: 4335125

Strand: Reverse

Name: nfi

Synonym: YpsIP31758_3848

Alternate gene names: 153950857

Gene position: 4335125-4334421 (Counterclockwise)

Preceding gene: 153950025

Following gene: 153946823

Centisome position: 91.78

GC content: 52.48

Gene sequence:

>705_bases
ATGTTTGATACTAAAGCGCTACAGGCAGAGCAGCGGCAACGGGCATCGGAGATTAGTCTTCATGATGGCATTGATAACCA
GTTTGTGCGCTTTATTGCTGGAGCTGATGTCGGTTTTGAGCAGCATGGGGAGATAACCCGTGCCGCTATCGCTATTTTAC
GCTATCCCTCTCTGGCGCTGGTGGAATACCAGGTTGCCAGAGTGGCAACTTCTCTGCCTTATATCCCCGGTTTACTCTCT
TTTCGTGAATATCCAGCGCTGTTAGCCGCGTGGGCGCAGTTGCAGCAACGCCCTGATCTGATTCTGGTCGATGGGCAAGG
CATCGCTCATCCTCGCCGTCTCGGCGTTGCCAGCCATTTTGGGTTATTGGTCGATGTACCGACGATTGGTGTCGCTAAAA
GCCGCCTGTGTGGTGATTTCTTGCCACTGCATCAAGATGTTGGTGCGGTGCAGCCCTTGTTCGATAACGATGAGCAGCTT
GGTTGGGTATGGCGCAGTAAAATCCGCTGTAACCCACTGTTTATTTCGCCTGGGCATCGGGTGAGTGTTGGCAGTGCGCT
GGCATGGGTTCAACGTTGCATGGCGGGTTATCGTCTGCCTGAACCAACCCGCTGGGCGGATGCTATTGCTTCAAACCGTC
CGCAATTTCAACGTTGGCTGCGGAAAAATCCTGATTTTCTTGGCAAGCGTAGGGATATGATTTAA

Upstream 100 bases:

>100_bases
TACATGCGCTATCACGGCCTTATCATCAAAAATAATCAGCGTGTAGGATGGAGCCGATTGGTCAATGGTGAAGACTAAAT
CGAGGGTTGAGACTAAATCA

Downstream 100 bases:

>100_bases
TTTCGGTTACACTGCCGCGCAGATAATGAATAATGAGAACTCATCATGTTACGTAATCCGATTCATCTACGGCTCGAAAA
GCTGGAAAGTTGGCAACATC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MFDTKALQAEQRQRASEISLHDGIDNQFVRFIAGADVGFEQHGEITRAAIAILRYPSLALVEYQVARVATSLPYIPGLLS
FREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHFGLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQL
GWVWRSKIRCNPLFISPGHRVSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI

Sequences:

>Translated_234_residues
MFDTKALQAEQRQRASEISLHDGIDNQFVRFIAGADVGFEQHGEITRAAIAILRYPSLALVEYQVARVATSLPYIPGLLS
FREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHFGLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQL
GWVWRSKIRCNPLFISPGHRVSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI
>Mature_234_residues
MFDTKALQAEQRQRASEISLHDGIDNQFVRFIAGADVGFEQHGEITRAAIAILRYPSLALVEYQVARVATSLPYIPGLLS
FREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHFGLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQL
GWVWRSKIRCNPLFISPGHRVSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family

Homologues:

Organism=Homo sapiens, GI31542775, Length=195, Percent_Identity=38.4615384615385, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI257467548, Length=121, Percent_Identity=41.3223140495868, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI257467550, Length=121, Percent_Identity=42.1487603305785, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI87082357, Length=223, Percent_Identity=69.0582959641256, Blast_Score=324, Evalue=3e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NFI_YERP3 (A7FNH1)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402799.1
- ProteinModelPortal:   A7FNH1
- STRING:   A7FNH1
- GeneID:   5387519
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3848
- eggNOG:   COG1515
- HOGENOM:   HBG716360
- OMA:   CMRGYRL
- ProtClustDB:   PRK11617
- BioCyc:   YPSE349747:YPSIP31758_3848-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00801
- InterPro:   IPR007581

Pfam domain/function: PF04493 Endonuclease_5

EC number: =3.1.21.7

Molecular weight: Translated: 26305; Mature: 26305

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDTKALQAEQRQRASEISLHDGIDNQFVRFIAGADVGFEQHGEITRAAIAILRYPSLAL
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHCCCHHH
VEYQVARVATSLPYIPGLLSFREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCHHHHC
GLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQLGWVWRSKIRCNPLFISPGHR
CEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCC
VSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure
MFDTKALQAEQRQRASEISLHDGIDNQFVRFIAGADVGFEQHGEITRAAIAILRYPSLAL
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHCCCHHH
VEYQVARVATSLPYIPGLLSFREYPALLAAWAQLQQRPDLILVDGQGIAHPRRLGVASHF
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCHHHHC
GLLVDVPTIGVAKSRLCGDFLPLHQDVGAVQPLFDNDEQLGWVWRSKIRCNPLFISPGHR
CEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCEEEECCCCC
VSVGSALAWVQRCMAGYRLPEPTRWADAIASNRPQFQRWLRKNPDFLGKRRDMI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA