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The map label for this gene is intS [H]

Identifier: 153950830

GI number: 153950830

Start: 4620690

End: 4620962

Strand: Reverse

Name: intS [H]

Synonym: YpsIP31758_4097

Alternate gene names: 153950830

Gene position: 4620962-4620690 (Counterclockwise)

Preceding gene: 153949200

Following gene: 153947597

Centisome position: 97.83

GC content: 44.69

Gene sequence:

>273_bases
ATGCCACTATCAGATAGACAGATTCGCCGTGCTAAACCACAAGAAAAAGCCTATACCCTGAGTGACGGGCAAGGGCTATC
ACTCTTAATTGAACCTAACGGCAGTAAAGGGTGGCGCTTTCGCTATCGTTTTGCCGGTAAAGCCCGATTAATGTCATTAG
GGACTTATGATCTTGTTTCTCTTGCCGAGGCCCGTTGCAAAAGAAATACTCAACTTTGTCCTACAACAGCAAATAGACTA
GCTACATCAAACTTATCATCAAGCCACCAGTGA

Upstream 100 bases:

>100_bases
CATTTTCTGTCTGTGGTGTTCCGAGTTGATATCCTCAAATCTGCAACAAATGTGTATAGCAATGTGTATAGGATTTTATG
GTGATTTTTGAGCTATACAC

Downstream 100 bases:

>100_bases
GCCTCTTGAGGCCGAATAACCCCTAAATGTAAAAAGGCATCGTGCGATGCCTTTAAAGATTAATAATCACTCCATCTCAC
TTCATTTGAGCACTTAACGT

Product: phage integrase family site specific recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MPLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVSLAEARCKRNTQLCPTTANRL
ATSNLSSSHQ

Sequences:

>Translated_90_residues
MPLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVSLAEARCKRNTQLCPTTANRL
ATSNLSSSHQ
>Mature_89_residues
PLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVSLAEARCKRNTQLCPTTANRLA
TSNLSSSHQ

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=93, Percent_Identity=38.7096774193548, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI145693166, Length=79, Percent_Identity=45.5696202531646, Blast_Score=66, Evalue=4e-13,
Organism=Escherichia coli, GI1788974, Length=69, Percent_Identity=50.7246376811594, Blast_Score=62, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 10087; Mature: 9955

Theoretical pI: Translated: 10.99; Mature: 10.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVS
CCCCCCCHHHCCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHH
LAEARCKRNTQLCPTTANRLATSNLSSSHQ
HHHHHHHCCCCCCCCCCCHHCCCCCCCCCC
>Mature Secondary Structure 
PLSDRQIRRAKPQEKAYTLSDGQGLSLLIEPNGSKGWRFRYRFAGKARLMSLGTYDLVS
CCCCCCHHHCCCCCCEEEECCCCCCEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHH
LAEARCKRNTQLCPTTANRLATSNLSSSHQ
HHHHHHHCCCCCCCCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]