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The map label for this gene is yneJ [H]

Identifier: 153950814

GI number: 153950814

Start: 2473776

End: 2474738

Strand: Reverse

Name: yneJ [H]

Synonym: YpsIP31758_2150

Alternate gene names: 153950814

Gene position: 2474738-2473776 (Counterclockwise)

Preceding gene: 153947384

Following gene: 153949662

Centisome position: 52.39

GC content: 50.36

Gene sequence:

>963_bases
ATGACACTGAAACAGCTTGAGGCCTTTTATTGGGCGGCCAGATGTTCTAATTTTTCCATTGCTGCTGAGCGGCTGCATAT
TTCTATCTCTTCCTTATCCAAGCGAATAGTCGAGCTGGAAGCGTCACTGGGGTGTACTCTTTTTGACCGCTCTGGATACC
GGGCGGTGTTAACGGAGACCGGTCAGAACCTGCTACCGCGCGCATTTGCATTGTTGGAGTCTGCTGCTGAAATCCGCAAT
ACTCTGGGCGCAAGCGAAGGTTTGTCTGGCATCTGTAATTTTGGCGTTGGAGAGTTGAGCGCTCTGACGTGGCTGCCGAG
TTTTATTGCTTCAGCCCGTTTGCTTTATCCGAAGTTGGTTTTAGCGCCACATGTTGATGTTGGTGCGGCTCTTGAAGGAC
GAGTTAATAAGGGCGAAATGGATTTTGCGATCATTGCGGGGCGTTCTTCTCGGCAGAATATTTTGTCGTTGCCCATTGCT
CAGGCGCAGTTTGTCTGGATGGCATCGCCGACGTTAGCAAATACTGTCGGTACTGCCGACAGTGCGTTACGGCAGGCGTT
TCCTCTGATTACACTCCCGCCAGGAGCGGGTTCTAGCCGGATTATCGACGCTTGGCTCATGGCATATGGAATTGCCTCGG
TGGCCCGTATCAATTGCAACAGTTGGGGGGCCATTGTGGGTATGCTCATTGCGTCAGTCGGCATCGGCATTCTGCCAACA
AGTTGGGCTGAAAAGCTTTCCCTACACAATCAACTGGAATATCTTCCTTGTTCTGAACCTTTAGCAGCATTGAATTACTC
CTTCCAATGGCGGCGTGATGATCCACGCCCACTAATCAGAAAAATGCGTGTGTTGGTTGCCGACCTGGCCGATTTCTCGC
TAAATAGCCCACTGCCACTAAATAATTCACTGCCGCTACATCATTCACTACCGCTACATCATTCACTACCACAACGTGAT
TAA

Upstream 100 bases:

>100_bases
GGTAAAAATAAAATTCAGATTAGAAGGCAATTATTGATATAGAAAGCGAATGTTTTGATATAAATTAATTGACAAAAATA
GAACAAACCAGAACAAGAAT

Downstream 100 bases:

>100_bases
TTTAGTGTGCAAATTCCGCATACATTTAGCTATTAGGTGGTAAGTTGGTCACCTTCAATAATCGCAATCCAAGGATAGAC
ATAAATAGTGTCGTCAACGA

Product: LysR family substrate binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTLKQLEAFYWAARCSNFSIAAERLHISISSLSKRIVELEASLGCTLFDRSGYRAVLTETGQNLLPRAFALLESAAEIRN
TLGASEGLSGICNFGVGELSALTWLPSFIASARLLYPKLVLAPHVDVGAALEGRVNKGEMDFAIIAGRSSRQNILSLPIA
QAQFVWMASPTLANTVGTADSALRQAFPLITLPPGAGSSRIIDAWLMAYGIASVARINCNSWGAIVGMLIASVGIGILPT
SWAEKLSLHNQLEYLPCSEPLAALNYSFQWRRDDPRPLIRKMRVLVADLADFSLNSPLPLNNSLPLHHSLPLHHSLPQRD

Sequences:

>Translated_320_residues
MTLKQLEAFYWAARCSNFSIAAERLHISISSLSKRIVELEASLGCTLFDRSGYRAVLTETGQNLLPRAFALLESAAEIRN
TLGASEGLSGICNFGVGELSALTWLPSFIASARLLYPKLVLAPHVDVGAALEGRVNKGEMDFAIIAGRSSRQNILSLPIA
QAQFVWMASPTLANTVGTADSALRQAFPLITLPPGAGSSRIIDAWLMAYGIASVARINCNSWGAIVGMLIASVGIGILPT
SWAEKLSLHNQLEYLPCSEPLAALNYSFQWRRDDPRPLIRKMRVLVADLADFSLNSPLPLNNSLPLHHSLPLHHSLPQRD
>Mature_319_residues
TLKQLEAFYWAARCSNFSIAAERLHISISSLSKRIVELEASLGCTLFDRSGYRAVLTETGQNLLPRAFALLESAAEIRNT
LGASEGLSGICNFGVGELSALTWLPSFIASARLLYPKLVLAPHVDVGAALEGRVNKGEMDFAIIAGRSSRQNILSLPIAQ
AQFVWMASPTLANTVGTADSALRQAFPLITLPPGAGSSRIIDAWLMAYGIASVARINCNSWGAIVGMLIASVGIGILPTS
WAEKLSLHNQLEYLPCSEPLAALNYSFQWRRDDPRPLIRKMRVLVADLADFSLNSPLPLNNSLPLHHSLPLHHSLPQRD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=279, Percent_Identity=26.1648745519713, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788481, Length=215, Percent_Identity=31.6279069767442, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788748, Length=244, Percent_Identity=29.0983606557377, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=25.4098360655738, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34725; Mature: 34593

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKQLEAFYWAARCSNFSIAAERLHISISSLSKRIVELEASLGCTLFDRSGYRAVLTET
CCHHHHHHHHHHHHCCCCCEEHHHHEEEHHHHHHHHHHHHHHCCEEEEECCCCEEEEECC
GQNLLPRAFALLESAAEIRNTLGASEGLSGICNFGVGELSALTWLPSFIASARLLYPKLV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAPHVDVGAALEGRVNKGEMDFAIIAGRSSRQNILSLPIAQAQFVWMASPTLANTVGTAD
CCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCHHHCCCCCCEEEEEEECCHHHHHHCCHH
SALRQAFPLITLPPGAGSSRIIDAWLMAYGIASVARINCNSWGAIVGMLIASVGIGILPT
HHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCC
SWAEKLSLHNQLEYLPCSEPLAALNYSFQWRRDDPRPLIRKMRVLVADLADFSLNSPLPL
HHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
NNSLPLHHSLPLHHSLPQRD
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLKQLEAFYWAARCSNFSIAAERLHISISSLSKRIVELEASLGCTLFDRSGYRAVLTET
CHHHHHHHHHHHHCCCCCEEHHHHEEEHHHHHHHHHHHHHHCCEEEEECCCCEEEEECC
GQNLLPRAFALLESAAEIRNTLGASEGLSGICNFGVGELSALTWLPSFIASARLLYPKLV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAPHVDVGAALEGRVNKGEMDFAIIAGRSSRQNILSLPIAQAQFVWMASPTLANTVGTAD
CCCCCCCCCHHCCCCCCCCCCEEEEECCCCCCHHHCCCCCCEEEEEEECCHHHHHHCCHH
SALRQAFPLITLPPGAGSSRIIDAWLMAYGIASVARINCNSWGAIVGMLIASVGIGILPT
HHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCCC
SWAEKLSLHNQLEYLPCSEPLAALNYSFQWRRDDPRPLIRKMRVLVADLADFSLNSPLPL
HHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
NNSLPLHHSLPLHHSLPQRD
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]