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The map label for this gene is cspD [H]

Identifier: 153950801

GI number: 153950801

Start: 2963769

End: 2964032

Strand: Direct

Name: cspD [H]

Synonym: YpsIP31758_2609

Alternate gene names: 153950801

Gene position: 2963769-2964032 (Clockwise)

Preceding gene: 153947516

Following gene: 153948428

Centisome position: 62.75

GC content: 46.21

Gene sequence:

>264_bases
ATGGAGACGGGTACTGTTAAATGGTTCAACAACGCCAAAGGTTTTGGCTTTATTTGCCCTGAGGGCGGTGGCGAAGACAT
TTTCGCTCACTATTCAACTATCCAGATGGATGGTTACCGAACATTAAAAGCCGGACAAATAGTTAACTTTGATGTTCATG
AAGGCCCTAAAGGGAACCATGCTAGCCTGATTGTTCCGCTAGAGTTAAATAGTGTACCACCGTTAGACGGTGTATCACCG
CCGGAACTCGCTATACTGGCCTGA

Upstream 100 bases:

>100_bases
GTATTTTTGTGAGCTGTTGGAGTTTTAACCAACTTGCGCTCACTTTAACCAGTGATTACCTCATCTCAAACTAGAATAGC
CTTGCGAGGGACGTAGAAGT

Downstream 100 bases:

>100_bases
CACCACGTGGCCTGCCAGCCATATCCAAAGATATCTTTGAGTTGCGTTAACTGCGCTGTTCATCGCAATTCAGCGTATTG
TGATTCATACCTGTACTTTG

Product: cold shock domain-containing protein CspD

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
METGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQIVNFDVHEGPKGNHASLIVPLELNSVPPLDGVSP
PELAILA

Sequences:

>Translated_87_residues
METGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQIVNFDVHEGPKGNHASLIVPLELNSVPPLDGVSP
PELAILA
>Mature_87_residues
METGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQIVNFDVHEGPKGNHASLIVPLELNSVPPLDGVSP
PELAILA

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=71, Percent_Identity=87.3239436619718, Blast_Score=137, Evalue=1e-34,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=51.5625, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=52.3076923076923, Blast_Score=75, Evalue=6e-16,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=53.968253968254, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=47.6923076923077, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1788126, Length=55, Percent_Identity=54.5454545454545, Blast_Score=72, Evalue=9e-15,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=50.7692307692308, Blast_Score=71, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 9327; Mature: 9327

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQIVNFDVHEGPKGNH
CCCCEEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCCEEECCCEEEEEECCCCCCCCC
ASLIVPLELNSVPPLDGVSPPELAILA
CEEEEEEECCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
METGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQIVNFDVHEGPKGNH
CCCCEEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCCEEECCCEEEEEECCCCCCCCC
ASLIVPLELNSVPPLDGVSPPELAILA
CEEEEEEECCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]