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The map label for this gene is greB [H]

Identifier: 153950771

GI number: 153950771

Start: 4466115

End: 4466630

Strand: Reverse

Name: greB [H]

Synonym: YpsIP31758_3981

Alternate gene names: 153950771

Gene position: 4466630-4466115 (Counterclockwise)

Preceding gene: 153950134

Following gene: 153947217

Centisome position: 94.57

GC content: 48.84

Gene sequence:

>516_bases
ATGGCAAAAAGTAACTACATTACCCGCGAGGGCTGGCAGGCGCTGGACCGTGAATTGCATTATCTGTGGCGAGAAGAGCG
CCCGGTCGTCACTCAGGCGGTTTCTGAAGCCGCTGCTATGGGTGACCGTTCAGAAAATGCTGAATATATTTATGGAAAGA
AGCGGCTGAGAGAAATCGATCGCCGGGTACGCTTTCTTGCCAAGCGCCTTGAAGTGCTGAAAATTGTTGACCCTGATCCT
CGCCAGGAAGGTAAAGTCTTTTTTGGGGCCTGGGTGCGGGTAGAAAATGAGCAAGAAGAGCAACGCATCTTCCGTTTGGT
GGGGCCAGATGAGTTTGACCCGGCCAAAAAATGGATTTCAATTGATTCACCGGTCGCCCGTGCATTGATAGGGAAACAAG
TCGATGACGAGGTCACGGTGCAAACGCCTAATGGCGAGGCAACCTATTGGATATTAGAGATTCGTTATCGGCCATTTGAT
GAAGATAGGCCATTTAATAAAGATATTGATAATTAG

Upstream 100 bases:

>100_bases
AGTTATTATAATGAAAAATGGCTAACTTGATGAAAGATAACGATTATGGTGAAGATAACAACGATGATAAAAATAGCGAC
GATGGCAAAAAGTAACGATT

Downstream 100 bases:

>100_bases
GCGTTTGATAATTAAGCATACAAATAAGCTTTGTAACAATTTCAACTAGAATTTATACCATTAATAACTGTCTGTTAGAG
TAAGTTTTTCTAACAATGTG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDP
RQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFD
EDRPFNKDIDN

Sequences:

>Translated_171_residues
MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDP
RQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFD
EDRPFNKDIDN
>Mature_170_residues
AKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREIDRRVRFLAKRLEVLKIVDPDPR
QEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWISIDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDE
DRPFNKDIDN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=60.3896103896104, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=35.5263157894737, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20213; Mature: 20082

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREID
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
RRVRFLAKRLEVLKIVDPDPRQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWIS
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHCC
IDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDEDRPFNKDIDN
CCCHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKSNYITREGWQALDRELHYLWREERPVVTQAVSEAAAMGDRSENAEYIYGKKRLREID
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHH
RRVRFLAKRLEVLKIVDPDPRQEGKVFFGAWVRVENEQEEQRIFRLVGPDEFDPAKKWIS
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCHHHHCC
IDSPVARALIGKQVDDEVTVQTPNGEATYWILEIRYRPFDEDRPFNKDIDN
CCCHHHHHHHCCCCCCCEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]