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The map label for this gene is leuD

Identifier: 153950749

GI number: 153950749

Start: 3833894

End: 3834496

Strand: Direct

Name: leuD

Synonym: YpsIP31758_3408

Alternate gene names: 153950749

Gene position: 3833894-3834496 (Clockwise)

Preceding gene: 153949465

Following gene: 153950767

Centisome position: 81.17

GC content: 44.61

Gene sequence:

>603_bases
ATGGCAAAATTTATTCAACACATTGGTTTGGTTGCGCCGTTAGATGCAGCAAACGTCGATACCGACGCGATTATCCCTAA
GCAGTTTTTGCAAAAAGTGACTCGCACGGGCTTTGGCCAACACCTGTTTAACGATTGGCGTTTTTTGGATGATGCCGGCA
AGGTACCGAATCCTGATTTTGTCCTGAACCTGCCTCGCTATCAGGGTGCCACTATTTTGTTAGCCCGTGAGAATTTTGGC
TGTGGCTCCTCACGTGAACACGCACCATGGGCGCTGACGGATTTTGGTTTTAAAGTGGTTATCGCCCCGAGTTTTGCTGA
TATTTTTTATGGTAACGCGTTTAATAACCAATTATTACCGGTGACATTAAGCGAAGCGGATGTTGATACCTTATTCCAAT
TGGTTAAAGAGAATGAAGGTATTGAGTTTGTGGTTGATCTCGAGCAGCAAACGGTTAATGCCGGTGGCAAAAGCTATGCT
TTCGAAATCGATCCTTTCCGCCGCCACTGTATGATTAATGGGTTGGATAGCATTGGCTTGACGTTGCAGCATGAACACAA
TATCTCAGCATACGAAAAACAACAGCCTGAATTTCTACGTTAA

Upstream 100 bases:

>100_bases
ATTTGGTCAGCCCAGCCATGGCCGCCGCTGCCGCCGTCAGTGGTCATTTTGCTGATGTTCGCGAATTATCCGCCACCACC
CACTAACCGGAGACACCACC

Downstream 100 bases:

>100_bases
ATAACCGTTAAGGCCAGCAGAACTAACATGCCAGCCTGTTTAGTAAATATATCGTTATTTTTAGTAGTAATAGATTGTGA
GTCATTAATAGATTGTAAGT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MAKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDFVLNLPRYQGATILLARENFG
CGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLPVTLSEADVDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYA
FEIDPFRRHCMINGLDSIGLTLQHEHNISAYEKQQPEFLR

Sequences:

>Translated_200_residues
MAKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDFVLNLPRYQGATILLARENFG
CGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLPVTLSEADVDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYA
FEIDPFRRHCMINGLDSIGLTLQHEHNISAYEKQQPEFLR
>Mature_199_residues
AKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDFVLNLPRYQGATILLARENFGC
GSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLPVTLSEADVDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYAF
EIDPFRRHCMINGLDSIGLTLQHEHNISAYEKQQPEFLR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=80.2030456852792, Blast_Score=334, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6321429, Length=203, Percent_Identity=52.7093596059113, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_YERP3 (A7FM87)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402365.1
- ProteinModelPortal:   A7FM87
- SMR:   A7FM87
- STRING:   A7FM87
- GeneID:   5387846
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3408
- NMPDR:   fig|349747.3.peg.3306
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   YPSE349747:YPSIP31758_3408-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22458; Mature: 22326

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDF
CHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCE
VLNLPRYQGATILLARENFGCGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLP
EEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCEEE
VTLSEADVDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYAFEIDPFRRHCMINGLDSIGL
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCE
TLQHEHNISAYEKQQPEFLR
EEEECCCCCHHHCCCCCCCC
>Mature Secondary Structure 
AKFIQHIGLVAPLDAANVDTDAIIPKQFLQKVTRTGFGQHLFNDWRFLDDAGKVPNPDF
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCCCE
VLNLPRYQGATILLARENFGCGSSREHAPWALTDFGFKVVIAPSFADIFYGNAFNNQLLP
EEECCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCEEE
VTLSEADVDTLFQLVKENEGIEFVVDLEQQTVNAGGKSYAFEIDPFRRHCMINGLDSIGL
EEECCCCHHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEECHHHHHHHHCCCCCCCE
TLQHEHNISAYEKQQPEFLR
EEEECCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA