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The map label for this gene is rlmI

Identifier: 153950547

GI number: 153950547

Start: 2862314

End: 2863504

Strand: Direct

Name: rlmI

Synonym: YpsIP31758_2531

Alternate gene names: 153950547

Gene position: 2862314-2863504 (Clockwise)

Preceding gene: 153948047

Following gene: 153947166

Centisome position: 60.6

GC content: 47.19

Gene sequence:

>1191_bases
ATGACCGTACGCCTTATTCTTGCTAAAGGACGTGAAAAATCCTTACTTCGTCGCCACCCATGGATTTTCTCTGGGGCGGT
TCAACGCCTTGAAGGTGATGCACTTTCCGGTGAAACCATAGATATCCTTGATAGTCAGGGAAAATGGTTGGCCCGTGCCG
CCTACTCGCCTGAATCACAAATTTTGGCACGAGTCTGGACGTTCCAACAAGATGAGGTTATAGATTGCGCATTCTTTATT
CGCCGCCTACAACAGGCTCAGAATTGGCGAGATTGGCTGGCACAGCGTGATGGCCTTAATGGCTACCGCCTGATTGCCGG
TGAATCGGATGGCCTGCCGGGCATTACCATTGACCGTTTCCAGAATTTCTTGGTGTTACAGTTACTTTCTGCCGGTGCCG
AATATCAACGTGAAACGCTGGTCAGTGCGTTACAACATTGCTATCCAGAATGTTCTATTTATGATCGTTCCGATGTTTCT
GTCCGTAAAAAAGAAGGTTTGCCACTGACTCAAGGCCTTATCTGTGGCGAAATGCCACCAGCCCTGCTGCCAATTAGCGA
AAATGGCATGCAACTCTTCGTTGATATCCAGCAAGGTCATAAAACGGGTTTCTATTTAGATCAACGCGATAGCCGCTTAG
CGGCCCGTAATTATGCCAATGGCCGTCGTGTTTTGAACTGCTTCTCATACACGGGGGCTTTTGCTGTCGCCGCGTTGATG
GGCAATTGCCAACAGGTCATTAGTGTTGATACATCGCAATCCGTGTTAGATATTGCGAAACAAAACATTGAACTGAACCA
GTTGGATCTGAGCAAAACGGAGTTTGTCCGTGACGATGTATTCCAATTGTTGCGCAGCTATCGCGCTCAAGGGGAAAAAT
TCGACCTGATCATTATGGATCCACCTAAATTTGTTGAGAATAAAAGCCAATTAGCCAGTGCATGCCGTGGCTATAAAGAT
ATCAATATGTTGGCGATTCAATTACTGCGCCCTGGCGGTATCTTGCTCAGTTTCTCTTGTTCAGGTTTGATGCCGGTCGA
TTTATTCCAGAAAATTTTGGCCGATGCCGCGTTAGATGCAGGCCATGACATACAGTTTATAGAGCAGTTCCGCCAAGCTG
CGGATCACCCAGTGATCGCGGCTTATCCAGAAGGTTTATATCTAAAAGGGTTCGCGTGTCGGATAATGTAA

Upstream 100 bases:

>100_bases
TTGACATAATCATTCCCTCATCTGTGCGTGATAGTTTGCATTTTCTAGCACATACTCAGACAATATCGCTCTTTTTTACC
TAGATGGCAGCAACCTAATT

Downstream 100 bases:

>100_bases
CTTGAAATATCCTGCGACACCCTCATATACAAGACAGTGAAAAGTTCTCGGGAGGTTATCATGATCGCCAGCAAATTCGG
TATAGGCCAGCAGGTTCGTC

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQILARVWTFQQDEVIDCAFFI
RRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRFQNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVS
VRKKEGLPLTQGLICGEMPPALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMDPPKFVENKSQLASACRGYKD
INMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDAGHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRIM

Sequences:

>Translated_396_residues
MTVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQILARVWTFQQDEVIDCAFFI
RRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRFQNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVS
VRKKEGLPLTQGLICGEMPPALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMDPPKFVENKSQLASACRGYKD
INMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDAGHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRIM
>Mature_395_residues
TVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQILARVWTFQQDEVIDCAFFIR
RLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRFQNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVSV
RKKEGLPLTQGLICGEMPPALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALMG
NCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMDPPKFVENKSQLASACRGYKDI
NMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDAGHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRIM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=80.3030303030303, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=267, Percent_Identity=27.3408239700375, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMI_YERP3 (A7FJR8)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401496.1
- ProteinModelPortal:   A7FJR8
- SMR:   A7FJR8
- STRING:   A7FJR8
- GeneID:   5384323
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2531
- NMPDR:   fig|349747.3.peg.15
- eggNOG:   COG1092
- HOGENOM:   HBG411897
- OMA:   RAAYSPH
- ProtClustDB:   PRK15128
- BioCyc:   YPSE349747:YPSIP31758_2531-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01857
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614
- SMART:   SM00359

Pfam domain/function: PF10672 Methyltrans_SAM; SSF88697 PUA-like

EC number: =2.1.1.191

Molecular weight: Translated: 44496; Mature: 44364

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQ
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCHHH
ILARVWTFQQDEVIDCAFFIRRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
QNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVSVRKKEGLPLTQGLICGEMPP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCC
ALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
CEEEECCCCCEEEEEEECCCCCCCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMD
HCCHHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKSQLASACRGYKDINMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDA
CCHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
GHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRIM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC
>Mature Secondary Structure 
TVRLILAKGREKSLLRRHPWIFSGAVQRLEGDALSGETIDILDSQGKWLARAAYSPESQ
EEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCHHH
ILARVWTFQQDEVIDCAFFIRRLQQAQNWRDWLAQRDGLNGYRLIAGESDGLPGITIDRF
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
QNFLVLQLLSAGAEYQRETLVSALQHCYPECSIYDRSDVSVRKKEGLPLTQGLICGEMPP
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCC
ALLPISENGMQLFVDIQQGHKTGFYLDQRDSRLAARNYANGRRVLNCFSYTGAFAVAALM
CEEEECCCCCEEEEEEECCCCCCCEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
GNCQQVISVDTSQSVLDIAKQNIELNQLDLSKTEFVRDDVFQLLRSYRAQGEKFDLIIMD
HCCHHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKSQLASACRGYKDINMLAIQLLRPGGILLSFSCSGLMPVDLFQKILADAALDA
CCHHHCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
GHDIQFIEQFRQAADHPVIAAYPEGLYLKGFACRIM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA