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The map label for this gene is dusC

Identifier: 153950443

GI number: 153950443

Start: 3171791

End: 3172729

Strand: Reverse

Name: dusC

Synonym: YpsIP31758_2808

Alternate gene names: 153950443

Gene position: 3172729-3171791 (Counterclockwise)

Preceding gene: 153950333

Following gene: 153948552

Centisome position: 67.17

GC content: 48.78

Gene sequence:

>939_bases
ATGCGAGTAATACTGGCACCGATGGAAGGTGTATTAGATTCGCTGGTGCGTGAATTGCTCAGTGAAGTAAATGATTACGA
CCTTTGCATCACCGAGTTTTTGCGGGTGGTTGATCAACTGCTGCCTGCTAAATCTTTCTACCGCCTATGTCCTGAATTGC
ACAATCAAAGCCGTACCCAGTCTGGCACATTAGTACGTATCCAATTATTAGGCCAATACCCAGAGTGGCTGGCAGAAAAT
GCGGCTCGAGCGGTAGCGCTTGGTTCATATGGTGTTGATCTCAATTGCGGGTGTCCTTCAAAACTGGTTAACGGCAGTGG
CGGCGGGGCAACCTTGCTAAAAGATCCAGAACTGATTTATCAAGGTGCAAAAGCCATGCGTGCGGCTGTTCCGGCCCATC
TCCCCGTCACGGTAAAAATTCGTTTAGGTTGGGATTCTGGTGATCGCCAATTTGAAATTGCTGATGCCGTGCAGCAAGCG
GGGGCGACCGAATTGGCTGTTCATGGTCGAACCAAAGAAGATGGCTATCAAGCTGAACGGATTAACTGGCAAGCCATTGG
TGAGATTCGCCAGCGCCTGACGATTCCAGTTATTGCTAATGGTGAAATTTGGGATTACCAGAGCGCACAAGAGTGCATGA
AAGTGACCGGTTGTGATGCCGTGATGCTAGGCCGTGGCGCGCTGAATGTGCCTAATCTGAGCCGGGTGGTGAAGTATAAC
GAACCGCGTATGCCGTGGCTGGAAGTGGTCAAACTACTGCAAAAATATGTGCAGTTGGAAAAGCAGGGCGATACCGGATT
GTATCATGTTGCGCGCATCAAACAGTGGTTGGGGTATCTACGTAAAGAATATACCGAAGCGACAGATTTATTCGGTGAAA
TCCGCGCATTAAAAAACTCGAAGGATATTGCGCTGGCAATTCAGCGTATTAATCGTTAA

Upstream 100 bases:

>100_bases
TTATCCCCGTTTCCCTCGTATCATTGCCAGCCTTTTTTGACATTATACGATATAGAATTAGGCTGGCGGTCATGACAAAT
AAAGGCTCTGGATAATGAGT

Downstream 100 bases:

>100_bases
CTCAGGAAAATAATGTTTCAATTGAATAATAATGTGGTTGGAATATAAACGTATTGCCGAGAATAACGCTATGTTATTGT
TTTTTCTTGCGGCTGATAAA

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRVILAPMEGVLDSLVRELLSEVNDYDLCITEFLRVVDQLLPAKSFYRLCPELHNQSRTQSGTLVRIQLLGQYPEWLAEN
AARAVALGSYGVDLNCGCPSKLVNGSGGGATLLKDPELIYQGAKAMRAAVPAHLPVTVKIRLGWDSGDRQFEIADAVQQA
GATELAVHGRTKEDGYQAERINWQAIGEIRQRLTIPVIANGEIWDYQSAQECMKVTGCDAVMLGRGALNVPNLSRVVKYN
EPRMPWLEVVKLLQKYVQLEKQGDTGLYHVARIKQWLGYLRKEYTEATDLFGEIRALKNSKDIALAIQRINR

Sequences:

>Translated_312_residues
MRVILAPMEGVLDSLVRELLSEVNDYDLCITEFLRVVDQLLPAKSFYRLCPELHNQSRTQSGTLVRIQLLGQYPEWLAEN
AARAVALGSYGVDLNCGCPSKLVNGSGGGATLLKDPELIYQGAKAMRAAVPAHLPVTVKIRLGWDSGDRQFEIADAVQQA
GATELAVHGRTKEDGYQAERINWQAIGEIRQRLTIPVIANGEIWDYQSAQECMKVTGCDAVMLGRGALNVPNLSRVVKYN
EPRMPWLEVVKLLQKYVQLEKQGDTGLYHVARIKQWLGYLRKEYTEATDLFGEIRALKNSKDIALAIQRINR
>Mature_312_residues
MRVILAPMEGVLDSLVRELLSEVNDYDLCITEFLRVVDQLLPAKSFYRLCPELHNQSRTQSGTLVRIQLLGQYPEWLAEN
AARAVALGSYGVDLNCGCPSKLVNGSGGGATLLKDPELIYQGAKAMRAAVPAHLPVTVKIRLGWDSGDRQFEIADAVQQA
GATELAVHGRTKEDGYQAERINWQAIGEIRQRLTIPVIANGEIWDYQSAQECMKVTGCDAVMLGRGALNVPNLSRVVKYN
EPRMPWLEVVKLLQKYVQLEKQGDTGLYHVARIKQWLGYLRKEYTEATDLFGEIRALKNSKDIALAIQRINR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=30.3030303030303, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI8923374, Length=273, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=31.1475409836066, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI239788483, Length=186, Percent_Identity=31.1827956989247, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI239788462, Length=186, Percent_Identity=31.1827956989247, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788462, Length=311, Percent_Identity=83.2797427652733, Blast_Score=549, Evalue=1e-158,
Organism=Escherichia coli, GI1789660, Length=281, Percent_Identity=32.3843416370107, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=25.6329113924051, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=168, Percent_Identity=36.9047619047619, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=252, Percent_Identity=29.7619047619048, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=34.4155844155844, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=215, Percent_Identity=26.5116279069767, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=228, Percent_Identity=29.3859649122807, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=30.1369863013699, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585320, Length=218, Percent_Identity=31.651376146789, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=148, Percent_Identity=33.1081081081081, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920448, Length=148, Percent_Identity=33.1081081081081, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSC_YERPE (Q8ZGV2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0144
- RefSeq:   NP_670315.1
- RefSeq:   NP_992340.1
- RefSeq:   YP_002346213.1
- ProteinModelPortal:   Q8ZGV2
- SMR:   Q8ZGV2
- IntAct:   Q8ZGV2
- GeneID:   1147962
- GeneID:   1174024
- GeneID:   2766142
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1175
- KEGG:   ypk:y3015
- KEGG:   ypm:YP_0963
- HOGENOM:   HBG557545
- OMA:   VGGIDWC
- ProtClustDB:   PRK10550
- BioCyc:   YPES187410:Y3015-MONOMER
- BioCyc:   YPES214092:YPO1175-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 34851; Mature: 34851

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILAPMEGVLDSLVRELLSEVNDYDLCITEFLRVVDQLLPAKSFYRLCPELHNQSRTQ
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
SGTLVRIQLLGQYPEWLAENAARAVALGSYGVDLNCGCPSKLVNGSGGGATLLKDPELIY
CCCEEEEEECCCCHHHHHHHHHHEEEECCCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
QGAKAMRAAVPAHLPVTVKIRLGWDSGDRQFEIADAVQQAGATELAVHGRTKEDGYQAER
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCEEEECCCCCCCCCHHHH
INWQAIGEIRQRLTIPVIANGEIWDYQSAQECMKVTGCDAVMLGRGALNVPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWLEVVKLLQKYVQLEKQGDTGLYHVARIKQWLGYLRKEYTEATDLFGEIRALKNS
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KDIALAIQRINR
CHHHHHHHHHCC
>Mature Secondary Structure
MRVILAPMEGVLDSLVRELLSEVNDYDLCITEFLRVVDQLLPAKSFYRLCPELHNQSRTQ
CEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
SGTLVRIQLLGQYPEWLAENAARAVALGSYGVDLNCGCPSKLVNGSGGGATLLKDPELIY
CCCEEEEEECCCCHHHHHHHHHHEEEECCCCCEECCCCCHHHCCCCCCCCEEECCHHHHH
QGAKAMRAAVPAHLPVTVKIRLGWDSGDRQFEIADAVQQAGATELAVHGRTKEDGYQAER
HHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHCCCCEEEECCCCCCCCCHHHH
INWQAIGEIRQRLTIPVIANGEIWDYQSAQECMKVTGCDAVMLGRGALNVPNLSRVVKYN
CCHHHHHHHHHHEECEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWLEVVKLLQKYVQLEKQGDTGLYHVARIKQWLGYLRKEYTEATDLFGEIRALKNS
CCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KDIALAIQRINR
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430