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The map label for this gene is gspE [H]

Identifier: 153950397

GI number: 153950397

Start: 1143727

End: 1145238

Strand: Direct

Name: gspE [H]

Synonym: YpsIP31758_0954

Alternate gene names: 153950397

Gene position: 1143727-1145238 (Clockwise)

Preceding gene: 153949658

Following gene: 153950854

Centisome position: 24.21

GC content: 39.22

Gene sequence:

>1512_bases
ATGACAGATATTGAGTCTGAGTTCCATACTTTGCCATTAACGTTTAGTTGGTCAAAGAGTCATGGTGTGCTTATTCTGCC
TATGGCACAGGGCAGTCAGTTGATTTGTCGTAAATCAGCGACATTGGAGGCTATACTTGAAGGTAATCGGGTGGCTCATA
GTCCAATACTTTTTAATTTAGTTACTGATGAAGAGTTTGAAAATAATTTAACTGAACGCTACCAAAATAATTCGGCTGAC
CCATATCAGACAATGAGCGTTATCAGCAATGAGATCGATATTTACTCTTTTGCTAACGAATTATCTGACGATGATGATTT
ATTGGATGTAACTGATGAAGCACCTGTAATAAAATTAATCAATAGCATTCTGGTCGAAGCAATTAAAGAGTTAGCATCTG
ATATACACATAGAGTCTTTCGATAAAAAACTTACTGTAAGATTTCGTATTGATGGTGTGTTAAGGAAAATATTGGAATTA
CAGCGCCATGTTGCACTATTATTAGTGTCGCGTATAAAAGTTATGGCCAAATTAGATATTGCAGAAAAACGTATTCCACA
AGATGGGCGCATAGCTCTAAATTTAGCGGGTAGAGCATTGGATGTTCGTGTTTCGGTATTACCATCTAGCCATGGTGAAC
GGGTCGTTATGCGTTTGCTTGACAAAAATAGCATTAAATTGGATTTACCCTCATTAGGTATGTCGAAAGATAACTGTATT
TCTATGAATACGCAGGTACATAAACCCCATGGCATTATCTTGGTTGTTGGCCCAACGGGGTCGGGTAAAAGCACTACGTT
ATATGCTGCTTTAATGGGGATTGACGCGAATGAGAAAAATATTATGACTGTAGAAGACCCTATTGAGTTCGACATACCTG
GTATATCCCAAACGCAAGTAAACCCAAAAATTGAAATGACATTTGCTCGTAGCTTAAGGGCAATATTACGCCAAGATCCT
GACGTTATTCTTATCGGTGAAATTCGTGATATTGAAACGGCACAGATAGCGGTTCAAGCTTCTCTAACCGGCCATTTAGT
TCTATCTACATTACATGCCAACAGTGCGATAGGTGCAGTGACCAGACTGAAGGATATGAATGTAGAGGCATTTATGCTTT
CAAGTTCATTATTGGCCATCATATCTCAGCGGTTGGTTAGAAGATTATGTATAGCGTGTCGCCAAGAGTCGTCAGTGACG
GAGAAGGTATTACAACGTCTGAATATTGATGATAAATGCTCTTTGGTTGGTTCGGTGTACCGGGCTAAAGGTTGTAACAA
ATGTAATCTTACTGGTTATCGTGGACGTATTGCTCTCCATGAATTTCTCGTAGTGGATAACTTATTGCGGGGGGCTATTT
ATAAAGGACTAGGAGAGTTTGAACTGGCTAAATTAGCCAGTGGGGCTATAAACAGCCTTCTATCTGATGGGATAAGCAAA
GTCATTGCAGGATTAACCACTATAGAGGAACTGGTAAGAATATGCCAAGAGGATGATAATGGCTGTATTTAA

Upstream 100 bases:

>100_bases
TAGCCGATGATATAGTCCTATCAGATAAAAAATATTTAAGTGTTATTCCTAGTATTAGGAAAGATATAAATAATTTTTAT
ACACTGTTGGACTCAGAGCT

Downstream 100 bases:

>100_bases
ATATGTTGCCATCAGCAGAAGTGGAACCAAGATAACAGGTGATATTGATGCGGAAAACATACGTATAGCCCGTTATCTCC
TTTACAAAAAAAACATGCAT

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTDIESEFHTLPLTFSWSKSHGVLILPMAQGSQLICRKSATLEAILEGNRVAHSPILFNLVTDEEFENNLTERYQNNSAD
PYQTMSVISNEIDIYSFANELSDDDDLLDVTDEAPVIKLINSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILEL
QRHVALLLVSRIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLPSLGMSKDNCI
SMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEFDIPGISQTQVNPKIEMTFARSLRAILRQDP
DVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAVTRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVT
EKVLQRLNIDDKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAINSLLSDGISK
VIAGLTTIEELVRICQEDDNGCI

Sequences:

>Translated_503_residues
MTDIESEFHTLPLTFSWSKSHGVLILPMAQGSQLICRKSATLEAILEGNRVAHSPILFNLVTDEEFENNLTERYQNNSAD
PYQTMSVISNEIDIYSFANELSDDDDLLDVTDEAPVIKLINSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILEL
QRHVALLLVSRIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLPSLGMSKDNCI
SMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEFDIPGISQTQVNPKIEMTFARSLRAILRQDP
DVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAVTRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVT
EKVLQRLNIDDKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAINSLLSDGISK
VIAGLTTIEELVRICQEDDNGCI
>Mature_502_residues
TDIESEFHTLPLTFSWSKSHGVLILPMAQGSQLICRKSATLEAILEGNRVAHSPILFNLVTDEEFENNLTERYQNNSADP
YQTMSVISNEIDIYSFANELSDDDDLLDVTDEAPVIKLINSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILELQ
RHVALLLVSRIKVMAKLDIAEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLPSLGMSKDNCIS
MNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEFDIPGISQTQVNPKIEMTFARSLRAILRQDPD
VILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAVTRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVTE
KVLQRLNIDDKCSLVGSVYRAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAINSLLSDGISKV
IAGLTTIEELVRICQEDDNGCI

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=484, Percent_Identity=50.4132231404959, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=43.455497382199, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87082188, Length=207, Percent_Identity=40.0966183574879, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55417; Mature: 55286

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIESEFHTLPLTFSWSKSHGVLILPMAQGSQLICRKSATLEAILEGNRVAHSPILFNL
CCCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHCCCCEECCCEEEEE
VTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDVTDEAPVIKLI
ECCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEECCCCCHHHHHH
NSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVSRIKVMAKLDI
HHHHHHHHHHHHHCCCEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLPSLGMSKDNCI
HHHHCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEE
SMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEFDIPGISQTQV
ECCCEEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCC
NPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAV
CCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEEHHCCCHHHHH
TRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNIDDKCSLVGSVY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHH
RAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAINSLLSDGISK
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VIAGLTTIEELVRICQEDDNGCI
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDIESEFHTLPLTFSWSKSHGVLILPMAQGSQLICRKSATLEAILEGNRVAHSPILFNL
CCCCCCCCEEEEEEEECCCCCEEEEECCCCCEEEEECCCHHHHHHCCCCEECCCEEEEE
VTDEEFENNLTERYQNNSADPYQTMSVISNEIDIYSFANELSDDDDLLDVTDEAPVIKLI
ECCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEECCCCCHHHHHH
NSILVEAIKELASDIHIESFDKKLTVRFRIDGVLRKILELQRHVALLLVSRIKVMAKLDI
HHHHHHHHHHHHHCCCEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKRIPQDGRIALNLAGRALDVRVSVLPSSHGERVVMRLLDKNSIKLDLPSLGMSKDNCI
HHHHCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCEE
SMNTQVHKPHGIILVVGPTGSGKSTTLYAALMGIDANEKNIMTVEDPIEFDIPGISQTQV
ECCCEEECCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEECCCCEECCCCCCCCCC
NPKIEMTFARSLRAILRQDPDVILIGEIRDIETAQIAVQASLTGHLVLSTLHANSAIGAV
CCCEEHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCEEEEEEHHCCCHHHHH
TRLKDMNVEAFMLSSSLLAIISQRLVRRLCIACRQESSVTEKVLQRLNIDDKCSLVGSVY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHH
RAKGCNKCNLTGYRGRIALHEFLVVDNLLRGAIYKGLGEFELAKLASGAINSLLSDGISK
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VIAGLTTIEELVRICQEDDNGCI
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]