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The map label for this gene is bcsZ [H]

Identifier: 153950370

GI number: 153950370

Start: 4586251

End: 4587366

Strand: Reverse

Name: bcsZ [H]

Synonym: YpsIP31758_4070

Alternate gene names: 153950370

Gene position: 4587366-4586251 (Counterclockwise)

Preceding gene: 153949844

Following gene: 229294895

Centisome position: 97.12

GC content: 50.45

Gene sequence:

>1116_bases
ATGGTCGTCATGTTTAAACACTTAGCCAGCATGTTCCTGTTGCTGGCCAGTTTTAGTCTCGCAGCCGCCAGTAACTGGCC
CGCGTGGCAACAGTTCAAACAAGATTACATCAGTGAAGGGGGGCGGATCATTGACCCCGGTAGCCCCTCGAAAATAACCA
CCTCGGAAGGCCAGAGTTATGGGCTTTTTTTTGCATTGGTTGCGGATGATCAGCCGATGTTTGAGCGTTTATTGGCTTGG
ACAGAAAACAATCTGGCAGCCGGTGATCTCACTTCCCGCCTTCCCGCTTGGTTATGGGGGCAAAACTCACAAAATAACTG
GGATATTCTGGACCCTAATTCGGCCTCCGATGCGGATATCTTGATTGCCTACAACTTGCTGGAGGCTGGCAGGTTATGGG
GTAACCGCCGTTACCTGATTATGGGTACCTTATTACTGCAACGTATTGCGCAAGAAGAAGTCATGGATATTCCCGGCCTT
GGCCAGATGCTATTACCGGGAAAAATTGGTTTTAACGATGAGGATACCTGGCGTCTCAACCCAAGTTATTTACCGCCACA
ACTACTGGCACGATTTTCCTCCATAGACGGGCCTTGGGAAGCGATGGTAGAAGTGAATCAGCGTATGTGGCTGGAAACCG
CACCAAACGGTTTTTCGCCGGACTGGGTGGTCTGGCAGAAAGGTAAAGGCTGGCAGCCCGATACCATAAAACCGGATGTC
GGCAGTAACGATGCCATTCTGGTTTATCTGTGGGCGGGGATGCTGGCAATGGACAGCCCACAAAAAGCTGAATTGATTGC
GCGTTTTCAGCCAATGGCGGTAATCACTCAGCAGCAAGGCCTGCCACCGTTTACGACCAACAGCGACAATGGTAAAACTA
ACGGGGATGGGTCAGTGGGTTTTTCTGCGGCATTATTGCCCTTTTTAGCCAGCAGCCCAGAGCCATTTAATCAGCAAACA
CTGAATCTCCAACAGCGACGGGTACAAAATTCACCGCCTGGCGCTGATGATTATTACAGTGCTATTCTGACCCTGTTTGG
TCAGGGGTGGTTACAGCATCGTTATCATTTTACCCATCAGGGAGAGCTACAGCCCTCATGGCACCGTCAACGTTAA

Upstream 100 bases:

>100_bases
ACATGGTTTGATCAACATGGGTAAGTCGCGATTACCGATGGCACTTGTGATGTCTGATAACCTCTCGCCGGTTATCACCT
GATGAGAGGGAGTTATTCTG

Downstream 100 bases:

>100_bases
GATTTCAGGGTAGGGCGGATCTGGCCTAATGATAGGGAAATGCGCTTAAGGGATCACAACTTACTCGATGGGGACTAAAA
AGGGGCGTCTAAACACCAAG

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MVVMFKHLASMFLLLASFSLAAASNWPAWQQFKQDYISEGGRIIDPGSPSKITTSEGQSYGLFFALVADDQPMFERLLAW
TENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYNLLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGL
GQMLLPGKIGFNDEDTWRLNPSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSPDWVVWQKGKGWQPDTIKPDV
GSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGSVGFSAALLPFLASSPEPFNQQT
LNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQPSWHRQR

Sequences:

>Translated_371_residues
MVVMFKHLASMFLLLASFSLAAASNWPAWQQFKQDYISEGGRIIDPGSPSKITTSEGQSYGLFFALVADDQPMFERLLAW
TENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYNLLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGL
GQMLLPGKIGFNDEDTWRLNPSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSPDWVVWQKGKGWQPDTIKPDV
GSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGSVGFSAALLPFLASSPEPFNQQT
LNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQPSWHRQR
>Mature_371_residues
MVVMFKHLASMFLLLASFSLAAASNWPAWQQFKQDYISEGGRIIDPGSPSKITTSEGQSYGLFFALVADDQPMFERLLAW
TENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADILIAYNLLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGL
GQMLLPGKIGFNDEDTWRLNPSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSPDWVVWQKGKGWQPDTIKPDV
GSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGSVGFSAALLPFLASSPEPFNQQT
LNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQGELQPSWHRQR

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=370, Percent_Identity=53.7837837837838, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 41661; Mature: 41661

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVMFKHLASMFLLLASFSLAAASNWPAWQQFKQDYISEGGRIIDPGSPSKITTSEGQSY
CEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCC
GLFFALVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADI
EEEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCCCCCEECCCCCCCCEE
LIAYNLLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLN
EEEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEC
PSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSPDWVVWQKGKGWQPDTIKPDV
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
GSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGSVG
CCCCEEEEEEEHHHHHCCCCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCCCCCCCC
FSAALLPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCEECCCC
GELQPSWHRQR
CCCCCCCCCCC
>Mature Secondary Structure
MVVMFKHLASMFLLLASFSLAAASNWPAWQQFKQDYISEGGRIIDPGSPSKITTSEGQSY
CEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCC
GLFFALVADDQPMFERLLAWTENNLAAGDLTSRLPAWLWGQNSQNNWDILDPNSASDADI
EEEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCCCCCEECCCCCCCCEE
LIAYNLLEAGRLWGNRRYLIMGTLLLQRIAQEEVMDIPGLGQMLLPGKIGFNDEDTWRLN
EEEEHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEC
PSYLPPQLLARFSSIDGPWEAMVEVNQRMWLETAPNGFSPDWVVWQKGKGWQPDTIKPDV
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
GSNDAILVYLWAGMLAMDSPQKAELIARFQPMAVITQQQGLPPFTTNSDNGKTNGDGSVG
CCCCEEEEEEEHHHHHCCCCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCCCCCCCC
FSAALLPFLASSPEPFNQQTLNLQQRRVQNSPPGADDYYSAILTLFGQGWLQHRYHFTHQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCEECCCC
GELQPSWHRQR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]