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The map label for this gene is pheT [H]

Identifier: 153950288

GI number: 153950288

Start: 1985636

End: 1988023

Strand: Direct

Name: pheT [H]

Synonym: YpsIP31758_1717

Alternate gene names: 153950288

Gene position: 1985636-1988023 (Clockwise)

Preceding gene: 153947269

Following gene: 153948307

Centisome position: 42.04

GC content: 48.37

Gene sequence:

>2388_bases
ATGAAATTCAGTGAACTTTGGTTACGCGAATGGGTAAATCCAGCCATTAGCAGCGATGATTTAGCTCATCAGATTACCAT
GGCCGGGCTAGAGGTTGATGGTGTAGACGCGGTTGCCGGTGAGTTTAACGGCGTTGTTGTGGGTCATGTCGTGGAGTGTG
GGCAACATCCAAATGCAGATAAGCTGCGAGTGACCAAAATTGATGTCGGCGGTGATCGCCTCCTGGACATCGTTTGTGGT
GCACCTAATTGCCGTCAAGGCCTCAAAGTTGCAGTTGCAACGGTAGGGGCGGTATTACCGGGTGACTTTAAAATTAAAGC
CGCCAAACTGCGTGGTGAGTCTTCAGAAGGGATGCTGTGCTCTTTTTCTGAGCTGGCTATTTCAGACGATCATGATGGTA
TTATTGAATTGCCCGCAGATGCTCCCATTGGTGTCGATGTGCGTGAGTATTTGCACTTGGACGACAAGACGATTGAAATC
AGCGTAACGCCAAACCGTGCTGATTGTTTAGGGATTATTGGTGTCGCTCGTGATGTAGCTGTCGTTAATCAATTGCCATT
AACTGAACCGGATATGGCTGAAGTTGTGGCATCAATTAATGACACACTTCCTATTCGTGTCGATGCACCACAAGCTTGCC
CACGTTATCTGGGCCGTGTGGTGAAGGGAATTAATGTTAAGGCTGCGACACCCCTATGGATGCGTGAAAAATTGCGCCGT
TGTGGCATTCGTTCAATTGATCCTGTTGTCGATGTGACCAACTTTGTGTTGCTGGAGCTAGGGCAGCCAATGCATGCCTT
TGATCTGGATCGTATCAGTGGCGGGGTCATCGTTCGTCTGGCAACTGAAGGCGAAGAACTCACGCTATTAGATGGTACAA
AAGTCAAGTTGAACGCAGACACACTGGTTATTGCCGATCATCAGAAACCATTGGCGATGGCGGGTATCTTTGGTGGCGAA
CATTCTGGTGTCAATGAAGAAACCAGAAATGTATTGCTGGAGTCCGCGTTCTTCAATCCACTGTCTATTACTGGCCGAGC
ACGCCGCCATGGCTTACATACTGATGCCTCTCATCGTTATGAGCGTGGTGTCGACCCAGCGTTACAGCATAAAGCTATCG
AACGTGCGACACGTCTACTGATTGATATTTGTGGTGGGGAAGCCGGGCCGGTTATGGATGTGACGACAGCATCAGAACTA
CCCAAACAAGCCACTATCACATTGCGCCGTGAGAAGCTGGATCGCCTAATTGGTCATCATATTCCATCTGAACAGGTGAG
TGATATTTTGCGTCGCTTAGGATGCAAAGTGACTGAATGTGGTTCTGATTGGCAGGCTGTAGCACCGAGCTGGCGTTTCG
ATATGGCTATCGAAGAAGACCTGGTGGAAGAAGTCGCCCGTATTTATGGCTATAACAATATTCCAGATGTCCCCGTTAGA
GCTGATTTGGTAATGACTAAACACCGTGAAGCAAGCTTGTCGCTGAAGCGGGTAAAGACAGCTTTGGTGGATCGTGGTTA
TCAGGAAGCAATTACTTACAGTTTTGTTGATCCTAAAGTACAGGCGTTACTGCATCCACAACAGGAAGCCCTCATTTTAC
CAAATCCTATTTCAGTTGATATGTCCGCAATGCGTTTATCACTGCTAACGGGTTTGCTGTCGGCAGTCGTTTATAATCAG
AACCGTCAGCAATCTCGGCTGCGATTATTCGAAAGCGGTTTACGCTTTGTTCCTGATAATACGGCAGATTTAGGCATTAG
GCAGGATGTAATGCTGGCAGGTGTTATTGCTGGTCATCGTTACGATGAGCATTGGGATCTGGCGCGTCAGCCGATCGACT
TCTATGATTTGAAAGGTGATTTGGAAGCAATTCTGGAGCTTACCGGCAAATTATCTGATGTTCAGTTCCGGGCAGAAGCG
CATTCTGCTCTGCACCCTGGGCAGAGTGCTGCAATTTATTTAGCGGGTGAACACATTGGTTTCATTGGTGTAGTTCACCC
AGAATTGGAACGTAAGCTGGATCTGAATGGTCGAACTGTCGTGTTTGAAGTGCAGTGGAATAAGCTTGCAGACCGCGCTG
TGCCTCAGGCCAGAGAGATTTCTCGCTTCCCGGCGAACCGTCGTGATATCGCTGTTGTGGTGGCTGAAAACGTTCCCGCA
GAAGATATTTTGGCCGAGTGTAAGAAAGTTGGCGCAAATCAGGTAGTTGGCGTAAACTTGTTTGATGTGTACCGTGGCAA
GGGCGTAGCAGAGGGCTATAAGAGTCTGGCTATTAGTCTGGTGTTGCAGGATACCGCCCGTACACTGGAAGAAGAGGAGA
TCGCCGCTACCGTTGCAAAATGCGTAGAGGCATTAAAACAGCGATTCCAAGCATCCTTGAGGGATTGA

Upstream 100 bases:

>100_bases
GAACGTTTAACGATGTTACGTTACGGCGTCACCGATTTGCGTGCATTCTTCGAAAACGATCTGCGTTTCCTCAAACAGTT
TAAGTAAGGCAGGATATCTC

Downstream 100 bases:

>100_bases
ACCTATGGCGCTTACTAAAGCTGAAATGTCAGAACATCTGTTTGAAAAGCTAGGGCTTAGCAAACGAGATGCTAAAGATC
TGGTTGAGTTATTCTTCGAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNADKLRVTKIDVGGDRLLDIVCG
APNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLCSFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEI
SVTPNRADCLGIIGVARDVAVVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATEGEELTLLDGTKVKLNADTLVIADHQKPLAMAGIFGGE
HSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRYERGVDPALQHKAIERATRLLIDICGGEAGPVMDVTTASEL
PKQATITLRREKLDRLIGHHIPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVDMSAMRLSLLTGLLSAVVYNQ
NRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHRYDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEA
HSALHPGQSAAIYLAGEHIGFIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAKCVEALKQRFQASLRD

Sequences:

>Translated_795_residues
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNADKLRVTKIDVGGDRLLDIVCG
APNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLCSFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEI
SVTPNRADCLGIIGVARDVAVVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATEGEELTLLDGTKVKLNADTLVIADHQKPLAMAGIFGGE
HSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRYERGVDPALQHKAIERATRLLIDICGGEAGPVMDVTTASEL
PKQATITLRREKLDRLIGHHIPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVDMSAMRLSLLTGLLSAVVYNQ
NRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHRYDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEA
HSALHPGQSAAIYLAGEHIGFIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAKCVEALKQRFQASLRD
>Mature_795_residues
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNADKLRVTKIDVGGDRLLDIVCG
APNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLCSFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEI
SVTPNRADCLGIIGVARDVAVVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATEGEELTLLDGTKVKLNADTLVIADHQKPLAMAGIFGGE
HSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRYERGVDPALQHKAIERATRLLIDICGGEAGPVMDVTTASEL
PKQATITLRREKLDRLIGHHIPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVDMSAMRLSLLTGLLSAVVYNQ
NRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHRYDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEA
HSALHPGQSAAIYLAGEHIGFIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAKCVEALKQRFQASLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=83.5220125786164, Blast_Score=1346, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=383, Percent_Identity=22.4543080939948, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 87174; Mature: 87174

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNAD
CCHHHHHHHHHCCCCCCCCHHHHHHEEECCEECCCHHHHCCCCCEEEEEHHHCCCCCCCC
KLRVTKIDVGGDRLLDIVCGAPNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLC
CEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEISVTPNRADCLGIIGVARDVA
EHHHHEECCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
HHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATEGEELTLLDGTKVKLNAD
CCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHCCCCEEEEEECCCCEEEEECCCEEEECCC
TLVIADHQKPLAMAGIFGGEHSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRY
EEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCHHHH
ERGVDPALQHKAIERATRLLIDICGGEAGPVMDVTTASELPKQATITLRREKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCHHEEEEHHHHHHHHHHCC
IPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
CCHHHHHHHHHHHCCEEEECCCCCHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCE
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVD
EEEEEECCCHHHHHHHHHHHHHHHCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCC
MSAMRLSLLTGLLSAVVYNQNRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
YDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEAHSALHPGQSAAIYLAGEHIG
CCCCHHHHCCCCCEEECCHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCEEEEEECCCEE
FIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEEECHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCH
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAK
HHHHHHHHHCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSELWLREWVNPAISSDDLAHQITMAGLEVDGVDAVAGEFNGVVVGHVVECGQHPNAD
CCHHHHHHHHHCCCCCCCCHHHHHHEEECCEECCCHHHHCCCCCEEEEEHHHCCCCCCCC
KLRVTKIDVGGDRLLDIVCGAPNCRQGLKVAVATVGAVLPGDFKIKAAKLRGESSEGMLC
CEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEE
SFSELAISDDHDGIIELPADAPIGVDVREYLHLDDKTIEISVTPNRADCLGIIGVARDVA
EHHHHEECCCCCCEEEECCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VVNQLPLTEPDMAEVVASINDTLPIRVDAPQACPRYLGRVVKGINVKAATPLWMREKLRR
HHHCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDPVVDVTNFVLLELGQPMHAFDLDRISGGVIVRLATEGEELTLLDGTKVKLNAD
CCCCCCCHHHHHHHHHHHHCCCCCHHHCHHHCCCCEEEEEECCCCEEEEECCCEEEECCC
TLVIADHQKPLAMAGIFGGEHSGVNEETRNVLLESAFFNPLSITGRARRHGLHTDASHRY
EEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCCCHHHH
ERGVDPALQHKAIERATRLLIDICGGEAGPVMDVTTASELPKQATITLRREKLDRLIGHH
HCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCHHEEEEHHHHHHHHHHCC
IPSEQVSDILRRLGCKVTECGSDWQAVAPSWRFDMAIEEDLVEEVARIYGYNNIPDVPVR
CCHHHHHHHHHHHCCEEEECCCCCHHCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCE
ADLVMTKHREASLSLKRVKTALVDRGYQEAITYSFVDPKVQALLHPQQEALILPNPISVD
EEEEEECCCHHHHHHHHHHHHHHHCCHHHHHEEEECCHHHHHHHCCCCCEEECCCCCCCC
MSAMRLSLLTGLLSAVVYNQNRQQSRLRLFESGLRFVPDNTADLGIRQDVMLAGVIAGHR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
YDEHWDLARQPIDFYDLKGDLEAILELTGKLSDVQFRAEAHSALHPGQSAAIYLAGEHIG
CCCCHHHHCCCCCEEECCHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCEEEEEECCCEE
FIGVVHPELERKLDLNGRTVVFEVQWNKLADRAVPQAREISRFPANRRDIAVVVAENVPA
EEEEECHHHHHHCCCCCCEEEEEEECHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCH
EDILAECKKVGANQVVGVNLFDVYRGKGVAEGYKSLAISLVLQDTARTLEEEEIAATVAK
HHHHHHHHHCCCCCEEEEEHHHHHCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHH
CVEALKQRFQASLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]