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The map label for this gene is apbE [H]

Identifier: 153950199

GI number: 153950199

Start: 3558462

End: 3559499

Strand: Reverse

Name: apbE [H]

Synonym: YpsIP31758_3160

Alternate gene names: 153950199

Gene position: 3559499-3558462 (Counterclockwise)

Preceding gene: 153949744

Following gene: 153950664

Centisome position: 75.36

GC content: 47.98

Gene sequence:

>1038_bases
GTGGTTGGGGCGATTGTGCGCAAGTATATAACACGTTGTTTAATGTTGGTGATAGGGGGCCTGCTGCTGAGTGGCTGTGG
CCCTGAGCAGGTCAGCCTTGAAGGCAAAACGATGGGAACCTCCTATTCTATTAAGTATGTGAGTGATTCATCAACGGCTG
CCCCAGAGACGTTACAACAGGAAATAGACCTCATACTTGAAGAGGTTAATGACCAGATGTCGACTTATCGGCCACATTCT
GAATTGAGCCGCTTTAATCAAAGCCGAGAGGTGAATCAACCTTTCCCTGTATCGGCGGCGACGGCCAAAGTAGTACAAGA
GGCTATACGTATTAACCGTCTGACCGATGGTGCCTTAGATGTGACTGTGGGGCCGTTAGTCAATTTATGGGGTTTTGGCC
CTGAAGGGCGCCCGGATACTGTACCTACCGAGGATGAAATCACTCGGCGTCGTGCGTGGGTAGGGATTGATAAGCTGGCA
GTGGATAAGAATGCATTAATAAAACGTGTTCCTGAACTGTATGTTGATCTCTCTTCTATCGCCAAAGGCTACGGCGTCGA
TGTAGTAGCTGAATATCTGGAGGCGCAGAATATCAAGGATTATATGGTTGATATCGGGGGGGAAGTCCGTACCCGTGGTA
ACAATGGAGAAAATAAACCTTGGCGTATCGCCATTGAAAAGCCAGTCGCGGGTTCTACACAACGTGCCCAGGAAATTATT
GAACCAGGGCGGATGGCGGTAGCCACCTCTGGTGATTATCGTAATTATTTTGAGCAGGACGGGATCCGTTATTCTCATAC
TATTGATCCTGATACCGGGCGGCCTATTAACCACCGGTTAGTCTCTATCACTGTTTTAGACCCAAGCTGTATGACTGCTG
ATGGCCTCTCTACCGGGTTAGGTGTCCTTGGGCCAGAGCGGGGAATGGCATTAGCCAATTTATTGGGCATCCCGGTCTTG
ATGATAATCAAAACAGATGATGGGTTTGAAGAGCAGTATTCAGATGCATTTAAACCCTATCTACAAGTAAAACATTAG

Upstream 100 bases:

>100_bases
GCTGCAGTGATCAAGATGCTGAAAGATCTTGGTGTAGAAGACGAAAATATTATGCTTGATGATTTCGGTGGCTGATCCGG
TCATCACTCGGGAATTCACA

Downstream 100 bases:

>100_bases
CCAGGAGGGGATATGTTGACTGTCTTTATCGCATCCTTTGTATTTTTTCTGTTAGCTATAGCGGGAATGTCGCTAGGTTA
TATCGTCAAAAATAAAACCA

Product: thiamine biosynthesis lipoprotein apbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MVGAIVRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLILEEVNDQMSTYRPHS
ELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLA
VDKNALIKRVPELYVDLSSIAKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEII
EPGRMAVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGPERGMALANLLGIPVL
MIIKTDDGFEEQYSDAFKPYLQVKH

Sequences:

>Translated_345_residues
MVGAIVRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLILEEVNDQMSTYRPHS
ELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLA
VDKNALIKRVPELYVDLSSIAKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEII
EPGRMAVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGPERGMALANLLGIPVL
MIIKTDDGFEEQYSDAFKPYLQVKH
>Mature_345_residues
MVGAIVRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLILEEVNDQMSTYRPHS
ELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLA
VDKNALIKRVPELYVDLSSIAKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEII
EPGRMAVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGPERGMALANLLGIPVL
MIIKTDDGFEEQYSDAFKPYLQVKH

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=346, Percent_Identity=41.6184971098266, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37916; Mature: 37916

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGAIVRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHH
EIDLILEEVNDQMSTYRPHSELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALD
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
VTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNALIKRVPELYVDLSSI
EEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHH
AKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEII
HCCCCHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHHH
EPGRMAVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGL
CCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
GVLGPERGMALANLLGIPVLMIIKTDDGFEEQYSDAFKPYLQVKH
CCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVGAIVRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHH
EIDLILEEVNDQMSTYRPHSELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALD
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
VTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNALIKRVPELYVDLSSI
EEHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHH
AKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEII
HCCCCHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHHH
EPGRMAVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGL
CCCCEEEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCC
GVLGPERGMALANLLGIPVLMIIKTDDGFEEQYSDAFKPYLQVKH
CCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]