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The map label for this gene is mepA

Identifier: 153949997

GI number: 153949997

Start: 1645533

End: 1646360

Strand: Direct

Name: mepA

Synonym: YpsIP31758_1406

Alternate gene names: 153949997

Gene position: 1645533-1646360 (Clockwise)

Preceding gene: 153949519

Following gene: 153949068

Centisome position: 34.84

GC content: 53.02

Gene sequence:

>828_bases
ATGAAAAATTGGATAGTGGGTATGGTGGCATTGGTCACCATGGTCCCTGTCATGGCGGCCACGCCATGGCAAAAGATTAC
GCACCCGGTGGCGGGTTCTCCACAGTCAATCGGTGGCTTTGCTAATGGTTGCGTGATTGGCGCGCAGCCTTTGCCGCTCG
AGTCTGCAGACTATCAAGTGATGCGTTCGGATCAGCGCCGTTATTTTGGTCACCCTGATTTACTGAATTTTATTCACCGT
CTCAGTGCACAAGCTCATCAACAACAATTGGGCACGGTGTTGATTGGTGATATGGCGATGCCTGCAGGCGGGCGTTTTAG
CAGTGGGCATGCCAGCCACCAGTCTGGTTTGGATGTGGATATTTGGCTACAACTGCCAAAGCAGCGTTGGAGCCAGCAGC
AGTTATTGAAACCGCAGCCGATAGACTTGGTCGCGGTTGATGGCAAGCGGGTGATCCCCGCACTTTGGCAGCCACAAATT
GAGAGTTTGATCAAATTGGCCGCCAAAGATAACGATGTCACGCGTATCTTCGTCAATCCGGCAATTAAAAAGCAGCTTTG
TCTGGATGCAGGTGCCGATCGTCAATGGCTGCATAAAGTCCGGCCGTGGTTTGCTCATCGTGCACACATGCATGTGCGCT
TACGTTGTCCGGCCAATAGCCTAGAGTGTGTGGACCAAGACACTCCACCGCCGGGCGATGGCTGTGGTGCTGAGCTGGAA
AGCTGGTTCCAACCGCCTCCGCCAAGTGCTAAACCAGGTAAAACCTTGCCACCGCCATTACCGCCTTCTTGTCAGGCTTT
ACTGGATAACCACTTCGCTACGGAATAA

Upstream 100 bases:

>100_bases
TTGCAGAAGCGATGATGGCTATCGTACTTATGGATCACCTACTGCGCCAGCGCGCGCAGTGCGGCGATGTGGCTTCAGAC
GTTCCGCGCTGGTAGATAGC

Downstream 100 bases:

>100_bases
GTCATGGATTGGTTTACCCTTGGTCCTGCCGTTTTGGGGATACTCTTTGCCGTTGCATTATTAGCCGGGTTCATTGATTC
CATTGCCGGCGGGGGCGGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHR
LSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQI
ESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE

Sequences:

>Translated_275_residues
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHR
LSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQI
ESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
>Mature_275_residues
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHR
LSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQI
ESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=73.4126984126984, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEPA_YERPA (Q1C665)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_652002.1
- ProteinModelPortal:   Q1C665
- SMR:   Q1C665
- STRING:   Q1C665
- GeneID:   4122003
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_2091
- eggNOG:   COG3770
- HOGENOM:   HBG296858
- ProtClustDB:   PRK09429
- BioCyc:   YPES360102:YPA_2091-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01623
- InterPro:   IPR009045
- InterPro:   IPR005073
- Gene3D:   G3DSA:3.30.1380.10
- PIRSF:   PIRSF018455

Pfam domain/function: PF03411 Peptidase_M74; SSF55166 Hedgehog_sig_N

EC number: 3.4.99.- [C]

Molecular weight: Translated: 30293; Mature: 30293

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQV
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHH
MRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNP
EEEECCHHHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECH
AIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHH
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQV
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHH
MRSDQRRYFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNP
EEEECCHHHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECH
AIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHH
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA