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The map label for this gene is speE

Identifier: 153949983

GI number: 153949983

Start: 3769540

End: 3770430

Strand: Direct

Name: speE

Synonym: YpsIP31758_3354

Alternate gene names: 153949983

Gene position: 3769540-3770430 (Clockwise)

Preceding gene: 153949088

Following gene: 153950461

Centisome position: 79.81

GC content: 48.71

Gene sequence:

>891_bases
ATGTCACAGAAAGAACTATGGTATGAAACATTGCATGCCAACTTCGGCCAGTACTTTTCTGTTGAGAATGTATTGTACCG
CGAAAAAACCGAGCATCAGGACCTGGTGATTTTTGAAAACCCAGAGTTGGGCCGCGTAATGGCATTAGATGGTGTGGTGC
AAACGACAGAGCGTGATGAGTTTATCTACCACGAAATGATGACACACGTTCCTTTGCTGGCCCACGGGCAAGCAAAAAAA
GTGCTGATCATCGGCGGTGGCGATGGCGCAATGCTGCGAGAAGTCAGCCGCCACAAGAATATTGAGCAGATTACCATGGT
GGAAATTGACGCCGGGGTCGTAGAATTTTGCCGCCAGTATTTGCCTAATCATAGCGCGGGGGCGTATGACGATCCTCGTT
TCAAGCTAGTCATCGACGATGGTGTGAATTTCGTTAATCAGACCACTGAAAAGTTCGATGTCATCATTTCTGACTGTACC
GATCCTATCGGTCCTGGCGAAAGCCTGTTTACCTCTGTATTTTATGAGGGATGCGCCCGTAGCCTGAATGAGGGGGGGAT
CTTCGTTGCACAAAACGGCGTCTGTTTCCTACAACAGGATGAAGCCGTCAATAGCCATAACAAACTTAGCCACTATTTCA
GTGACGTTAGTTTTTATCAGGCCGCAATTCCAACGTATTACGGCGGTATTATGACCTTTGCCTGGGCGACACAGAACCCG
GCTCTGCGCCAGTTGGATCTCGCCACCTTGCAAAACCGCTTTGCCCAAGCTGGCTTGGCCTGCCGCTACTATAACCCAGC
GATTCATGTCGGCAGCTTTGCTTTACCACAGTATTTGCTAGATGCCTTAACGACGATACCCAAAGTCATTGGCGTCGATT
CAAGTGAATAA

Upstream 100 bases:

>100_bases
GCCTTTTGCCTGAAATTTGACCGTGCAACCTCAGGCAGAGTTGGCTATGATATTGCCCCCCATTTTTCATGGCTGTTACT
GCGAAGGAGAAGCCCCGAAC

Downstream 100 bases:

>100_bases
GAGCCGCTAGCAACGCAGTAGTGCCGAGTAAAAAAGGGATAAATGTTGAGTATTCATTTGTAAGGAGGTGAACTAAATTG
TCCAAGCTTAAACTGCACGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSQKELWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPELGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKK
VLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQYLPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCT
DPIGPGESLFTSVFYEGCARSLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE

Sequences:

>Translated_296_residues
MSQKELWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPELGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKK
VLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQYLPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCT
DPIGPGESLFTSVFYEGCARSLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE
>Mature_295_residues
SQKELWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPELGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKKV
LIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQYLPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCTD
PIGPGESLFTSVFYEGCARSLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNPA
LRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=288, Percent_Identity=36.4583333333333, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1786312, Length=288, Percent_Identity=80.5555555555556, Blast_Score=502, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI25154106, Length=270, Percent_Identity=39.2592592592593, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI212645616, Length=226, Percent_Identity=40.7079646017699, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=38.4615384615385, Blast_Score=202, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6325326, Length=275, Percent_Identity=35.2727272727273, Blast_Score=180, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24645443, Length=278, Percent_Identity=38.1294964028777, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=36.2790697674419, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_YERP3 (A7FM33)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402311.1
- ProteinModelPortal:   A7FM33
- SMR:   A7FM33
- STRING:   A7FM33
- GeneID:   5386069
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3354
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   VKHPLVE
- ProtClustDB:   PRK00811
- BioCyc:   YPSE349747:YPSIP31758_3354-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 33183; Mature: 33052

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKELWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPELGRVMALDGVVQTTERDE
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHCCCCHH
FIYHEMMTHVPLLAHGQAKKVLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQY
HHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCTDPIGPGESLFTSVFYEGCAR
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
HCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE
CHHHHHHHHHHHHHHHHCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQKELWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPELGRVMALDGVVQTTERDE
CCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEECCHHHCCCCHH
FIYHEMMTHVPLLAHGQAKKVLIIGGGDGAMLREVSRHKNIEQITMVEIDAGVVEFCRQY
HHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHSAGAYDDPRFKLVIDDGVNFVNQTTEKFDVIISDCTDPIGPGESLFTSVFYEGCAR
CCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SLNEGGIFVAQNGVCFLQQDEAVNSHNKLSHYFSDVSFYQAAIPTYYGGIMTFAWATQNP
HCCCCCEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
ALRQLDLATLQNRFAQAGLACRYYNPAIHVGSFALPQYLLDALTTIPKVIGVDSSE
CHHHHHHHHHHHHHHHHCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA